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According to the prior research , a highly efficient ext raction method was modified1 High2concent ration and DNA 2f ree RNA could be attained by using CTAB/ Guanidine Thiocyanate ext racion buffer1The total RNA can reach 1.7～2.0 of the OD260/ OD280 ratio with the yield of more than 015mg per gram of f resh tissues1 The method is suitable for high2quailty total RNA ext raction in leaves , root s , corollas , seeds and developing fibers.

本研究在前人方法的基础上对快速提取棉花组织总RNA 的方法进行了探讨。利用改进的CTAB 异硫氰酸胍裂解缓冲液，获得的RNA完整性好，无 DNA 污染，含量高，每克鲜组织能提取015mg 总RNA ， OD260/ OD280比值均在117～210之间。

The daily total metabolic rates were determined for 31 fish with closed respirometers. In addition, resting daily total metabolic rates were determined for 20fish by confining the fish into a cage within the respirometer. The fish weighed 72.9-133.3g and were fed different ration levels from starvation to 4% bw / day at 25℃.

在25℃不同日粮水平（饥饿─4％体重／d）条件下，采用封闭式呼吸仪测定了31尾鲇鱼（体重72.9─133.3g）的日总代谢率，然后，将鱼限制在呼吸室中的限动笼内测定了其中20尾鲇鱼的静止日总代谢率，并计算出了鲇鱼的特殊动力作用和活动代谢率。

The Clean Water State Revolving Fund and Drinking Water State Revolving Fund will receive a total of $6 billion - of that total $4 billion will be used help communities with water quality and wastewater infrastructure needs and $2 billion will be spent on drinking water infrastructure needs.

洁净水周转基金和饮用水周转基金将获得共60亿美元──其中40亿美元将用於帮助有水质问题的社区和废水基础设施，另外20亿美元将用在饮用水基础设施；其中12 亿美元一定会用於环保基础设施建设。

Methods: Our hospital otorhinolaryngology March 2004 to May 2007 the new hospitalized patients with cerebrospinal fluid rhinorrhea (a total of 32) were divided into the experimental group (16) systematically and psychological assessment, and strengthen psychological care; The control group (a total of 16) using traditional psychological guidance, and compared between the two groups of patients with different medical order compliance.

对我院耳鼻咽喉科2004年3月～2007年5月新入院的脑脊液鼻漏患者（共32名）进行随机分组；对实验组（共16名）系统地进行心理评估，并进行心理干预，对对照组（共16名）采用传统性心理指导，比较两组患者对医嘱依从性的差异。

As to the distributing structure of Russian household incomes and savings, a dramatic change has taken place in it right because of the radical political and economic reforms. The gap between the rich and poor has widened. In 1998, earnings by the wealthiest 10% of the total population are 13.3 times of that by the poorest 10% of the total.

此外，由于剧烈的社会政治和经济变革，俄罗斯居民收入和储蓄的分布结构也发生了巨大的变化：改革使居民贫富差距扩大，1998年俄罗斯10%最富裕的人的收入是10%最贫困者收入的13.3倍。

From the purchase of the demand, the pure investment demand in the current market environment accounted for only 6 percent, while the buyers immediately after the arrival of the demand for 56 percent of total demand, proving once again that there is still great demand for the rigid market space, followed by self - living and the need to invest 28 percent of total demand.

从购买需求来看，纯投资性需求在目前市场环境下仅占6%，而购房后立即入住的需求占总需求56%，再次证明了刚性需求仍有很大的市场空间，其次是自住兼投资的需求占总需求28%。

In total, Meazza scored 33 goals in 53 international appearances, a total that was surpassed only by the great Luigi Riva.

他一生中代表国家队参加了53场比赛，进了33个球，这一成绩只有伟大的luigi riva超过。

The purpose of this study is to evaluate various data processing methods, including logarithmic transformation, robust statistics, excluding outliers and Box-Cox transformation for evaluating soil total P content with normal distribution. The result showed that Box-Cox transformation was applied in order to achieve normality in the data set and to dampen the effect of outliers. Geostatistical analyses were carried out, including calculation of experimental variograms and model fitting. The best theoretical model for semivariogram of soil total P were spherical model.

结果表明，Box-Cox转化成功地使数据集服从正态分布并消弱了异常值的影响，应用地统计学分析方法进行了实验变异函数的计算和最适合模型的拟合，得出土壤全磷最好的理论模型为球状模型，随后用普通克立格估值方法绘制了土壤全磷的空间分布图。

From the viewpoint of the wireless networks, the sources of network-induced delay are identified for a class of WiNCS built on the WSN. Because the routing severely affect the total round-trip time delay in this WiNCS, a novel routing algorithm that can minimize the total round-trip time delay is proposed, the detailed implementation of the algorithm is introduced.

针对基于无线传感网络实现的无线网络控制系统，从网络通讯的角度分析了网络延时的组成，在此基础上，提出了一种实现最小网络延时的路由算法，对路由算法的设计和实现进行了详细叙述。

For the WiNCS based on the IEEE 802.15.4/Zigbee communication protocols stack, the different time delay components of the total round-trip time delay in this WiNCS are identified. The experimental studies on a WiNCS prototype testbed are used to analyze the impact of the various factors on the total round-trip time delay.

分析了利用IEEE 802.15.4／Zigbee通讯协议所实现无线网络控制系统中延时的组成，在此基础上，以实验手段探讨了不同网络因素对网络控制系统延时的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。