查询词典 total up

In light of the data that has statistic from Netease, agonistic time signing up is less than a week, the activation number signing up that comes from 45 key schools of countrywide is total already passed 6000 people.

从网易有道统计的数据来看，竞赛报名时间不到一周，来自全国45所重点院校的激活报名人数合计已过6000人。

As...as possible in total make oneself at home except for stay up end up with

与考纲上单词互为构词变化形式的单词视作应掌握单词。

The oak tree class forest distributes in our country is very broad, the fruitful in resources, is one of my king monarch sovereign broadleaf tree lumbers, its wooded area accounts for the national forest total area about 12%, stores up the quantity to approximately compose the lumber to store up the quantity 10%.

摘 要：栎类林在我国分布很广，资源丰富，是我国主阔叶树木材之一，其森林面积占全国森林总面积的12%左右，蓄积量约占木材蓄积量的10%。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

Results (1) The total RNA, reverse transcription product and fluorescence labeled cRNA were all of high quality;(2) There were 420 up-regulated genes and 552 down-regulated genes among 2-fold DEGs, including DKK1 (dickkopf homolog 1) which was 5-fold up-regulated;(3) The results of real time RT-PCR, using β-actin as an internal control, showed that the 2-ΔCt values of DKK1 in HCC, PCT and NLT were 0.089 504, 0.007 65 and 0.000 631 respectively.

结果 （1）配对组织总RNA质量高，反转录cRNA及荧光标记质量好；（2）2倍差异表达基因中，上调基因共420个，下调基因共552个，其中包括5倍上调基因DKK1；（3）以β-肌动蛋白为内对照的实时逆转录聚合酶链反应结果提示，DKK1在癌、癌旁和正常肝组织中的2-ΔCt值分别为0.089504、0.00765和

For call up the Chinese people We need dial three times, total 27 numbers, then dail-up to the receiver number, but on the dialogist's mobile phone the number is not pressented, or displaid "anonymith calling", some receivers will not like to connect it, because of anonymith…..

在芝加哥给咱们中国打电话先要分三次一共拨27个号码，然后才能拨对方电话号码，拨通后，对方手机上不显示芝加哥的电话号码，或者只显示为"匿名电话"，对方有的人会因为这个"匿名电话"而不愿接呢

For example, the below query retrieves the ID and Name from the Product entity, the total number of orders that have been made for the Product, and then sums up the total revenue value of each of the Product's orders

例如，如下的这个查询检索Product实体的ID和Name，该产品的订单总数，然后将每个产品的订单的利润加了起来。

For example, the below query retrieves the ID and Name from the Product entity, the total number of orders that have been made for the Product, and then sums up the total revenue value of each of the Product's orders

例如，以下查询从Product实体中获取ID 和 Name，此产品的订单总数，以及对每个产品订单总收入的合计

The author comes to the conclusions that: the size of LSP is large, and its growth rate is high; the sex ratio of LSP is higher than the total population and working-age population, and comparatively speaking, its age structure is younger and cultural-educational quality is lower; the LFPR of total population goes steadily up; the characteristics of age-specific LFPR are similar to other countries; the LFPR of illiterate and semi-illiterate is lowest, and among the other population the higher the educational level, the lower the LFPR; the regional differences in the size, age-sex structure and cultural quality of LSP are obvious, and so is the LFPR.

首先对中国劳动力供给人口和劳动力参与率进行了研究，主要结论如下：劳动力供给人口的总量大、增长快；劳动力供给人口的性别比高于总人口和劳动年龄人口，其年龄构成比较年轻，文化素质也较低；总人口劳动力参与率稳步上升，1978—1994年，中国总人口劳动力参与率的回归模型为：Bt=40.1611(t-1977)〓(R〓=0.9150)；中国分年龄组劳动力参与率的特征与其他国家相类似，在15岁及以上人口中，文盲半文盲人口的劳动力参与率最低，其他文化程度人口则是文化层次越高，劳动力参与率越低。

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。