查询词典 total step method

Methods A total RNA from ten cases of ovarian cancer and contral normal ovarian tissues were extracted by a single step method. The cDNA retro-transcribed from equal quantity mRNA derived from ten cases of ovarian carcinoma and normal ovarian tissues, which were labeled cDNA strand with Cy5 and Cy3 fluorescence as probes.

按一步法分别抽提10例人卵巢癌组织和对照正常卵巢组织的总RNA并纯化mRNA；分别从10例取等量的人卵巢癌组织和对照组织的mRNA分别混合后逆转录合成以Cy5和Cy3标记的cDNA一链做探针，混合后在含有8464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

Methods］ A total RNA from 10 cases withovarian papillary serous cystadenocarcinoma and normal ovarian tissues were extracted by a single step method of gene chip. The cDNA retro?鄄transcribed from equal quantity mRNA derived from 10 cases with ovarian papillary serous cystadenocarcinoma and normal ovarian tissues. The cDNA strand was labeled with fluorescent Cy5 and Cy3(normal ovarian epithelial cells) as probes.

方法］ 采用基因芯片技术，按一步法分别抽提10例卵巢浆液性乳头状囊腺癌患者的癌组织和正常卵巢组织的总RNA并纯化mRNA；逆转录合成以Cy5荧光标记卵巢癌细胞和Cy3荧光标记正常卵巢上皮细胞的cDNA作为探针，在含有8 464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

The full text is total to is divided into four chapter:Chapter 1, the relevant concept of the listed company results evaluation, that is the other the theories foundation of the chapter, and do the foul to the textual evaluation angle settle;Chapter 2, the results evaluation index sign and the index sign system, the original index sign system of comprehensive evaluation of information formation that that choice listed company publish, and introduced the in common use index sign to sieve to endow with the method of the power with index sign;Chapter 3, the comprehensive results evaluation method, this is the foundation that carries on chapter 4, that carry on theories in chapter 2 fusion, became two kinds of comprehensive evaluation methods, and carried on the des cription to the operation step of a method;Chapter 4, the substantial evidence is analytical, this is the point of the full text, the collections combines the data of tidy up the listed company, making use of two kinds of methods within chapter 3s respectively, carry on synthesize to rank, and get two kinds of methods to rank the result to look like the consistent conclusion.

全文共分四章：第一章，上市公司绩效评价的有关概念，该章是其它章节的理论基础，并对本文的评价角度做出界定；第二章，绩效评价指标及指标体系，该章选择上市公司披露的信息形成综合评价的原始指标体系，并介绍了常用的指标筛选和指标赋权的方法；第三章，综合绩效评价方法，这是进行第四章的基础，该章将第二章中的理论进行融合，形成了两种综合评价方法，并对每种方法的操作步骤进行了描述；第四章，实证分析，这是全文的重点，收集并整理上市公司的数据资料，分别利用第三章中的两种方法，进行综合排名，并得到两种方法排名结果近似一致的结论。

All single step method and CTAB method did not work well when banana total RNA was isolated, unless modified single step method was used, in which, polysaccharide was removed by aether extraction prior to RNA precipitation.

对于富含多糖的香蕉果肉，一步法和CTAB异硫氰酸胍法的效果均不理想；改良后的一步法中增加了乙醚去除多糖并获得了较好的效果。

Single step method worked well when total RNA in pea, rice, Broussonetia Papyrifera, Cycloru Parasiticus and Wedelia chinensis Osb.

发现一步法对豌豆、水稻和蟛蜞菊叶片总RNA的提取均有很好的效果。

The total RNA were extracted from CDV infected DK cells or the tissues of infected animals by single step method of acid guanidinium thiocyanate-phenol-chloroform extraction and was used for reverse transcription.

用异硫氰酸胍-酚-仿一步抽提法提取细胞总RNA进行反转录，再以此产物为模板进行PCR扩增，筛选出最佳扩增条件。

ShTat1.3 and ShTat1.5 are two variable antigen types of a cloning Anhui strain of Trypanosoma evansi.Total RNA of the two variable antigen types were extracted by acid guanidinium thiocyanate-phenol-chlorform single step method.

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1.3和ShTat1.5总RNA，在含有甲醛的琼脂糖凝胶电泳后，转印到硝酸纤维膜上。

It has selected for using the total mark method with long step of turning into in emulation , and it is analyzed and starts with mechanism, carried on rational simplifying to the model , reduces the model calculate time , thus it improves the practicability of the model.

仿真中选用了变步长的积分方法，并从机理分析入手，对模型进行了合理的简化，使得模型计算时间大大缩短，从而提高了模型的实用性。

Step by step method ; Total product price ; Profit averaging ; Feature Value.

总剩余；分配因素；生产价格；理论价格；迭代方法；利润率；价格体系

The results show that the SAPO–5 has a pillar-like morphology with the size of about 20 μm×5 μm×5 μm.The ratio of P:Al:Si is 1:1.19:0.67 in its crystal structure, the content of Si is high. The total acidimety fo the SAPO–5 is 578.0 μmol/g , hihger than that of prepared by one step crystalline hydrothermal method.And its initial catalysis character performance on normal butane isomerization reaction reach 24.8 %, higher than that of prepared by one step crystalline hydrothermal method.

实验结果表明：产物为柱状的磷酸硅铝分子筛，其晶粒尺寸大约为20 μm×5 μm×5 μm，其晶体结构中P：Al：Si＝1：1.19：0.67，硅含量较高；采用分段晶化方式制备的SAPO-5分子筛的总酸量为578.0 μmol/g高于一段晶化方式制备的SAPO-5分子筛的总酸量；采用分段晶化生成的SAPO-5分子筛的催化正丁烷异构化反应初始活性可达到24.8 %，远高于一段晶化制备的SAPO-5分子筛的初始活性14.1 %。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失