查询词典 total differential

METHODS Low-dose Peth 0.01, 0.1 and 1 mgkg^(-1) were given iv 10 min before guinea pigs inhaled 75% cigarette smoke 60 mL. Then, the changes in airway resistance R(subscript aw, dynamic lung compliance C(subscript dyn and vascular permeability in airway tissues were observed. The total leukocyte and the differential leukocyte in bronchoalveolar lavage fluid and nitric oxid e content in BALF were investigated after guinea pigs given iv Peth 0.1 mgkg^(-1) 10 min before cigarette smoke inhalation and consecutively inhaled 360 mL smoke in 2 h.

观察Peth 0.01，0.1和1mgkg^(-1)对豚鼠自主吸入75%香烟烟雾（含25% O2）60mL后，气道阻力和肺动态顺应性变化的影响及气道组织血管通透性变化的影响；观察Peth 0.1mgkg^(-1)对豚鼠2h内分6次吸入（共360mL）75%浓度的香烟烟雾后，支气管肺泡灌洗液中白细胞总数和分类计数改变的影响，测定BALF中一氧化氮含量。

According to the energy conservation theory, BOM and CSIM4 were coupled. The BOM has no treatment on transmission solar radiation, which is of great importance when the model is adapted to Arctic Ocean. So the treatment was introduced to BOM. Through numerical test on different lead albedos, it was found that sea ice thickness is not so sensitive to lead albedo, which may be contribute to the lead occupies little ratio within multiyear sea ice pack. The reason of summer over-melt of arctic sea ice is the NCEP reanalysis downward solar radiation being larger than its reality. Then the arctic sea ice climate variability was simulated. Results showed that: simulated ice thickness change is in accord with the submarine investigated mean sea-ice draft changes. Simulated annually maximum ice thickness along the Eurasian continental oceans are closely related to the observed ones. The long-term mean simulated ice motion has the same features of the SSM/I derived ice motion. Sea ice extents in differential sub-regions have same trends comparing to the satellite passive-microwave data derived ones. Simulated ice concentration is closely related to the observed in the Arctic sub-regions. Sea ice flux through the Fram Strait involves ice concentration, motion and thickness. It is a composite criterion for sea ice model evaluation. The simulated ice area and volume export through the strait accord with the satellite derived or statistically reconstructed ones.(5) The simulated ice thickness climate variability and mean sea surface current of the coupled model were analyzed, results showed: the total ice volume in the Arctic Ocean has a significant decreasing trend. The volume variability is of a 10-year timescale oscillation, with two major periods of 12-13a and 18-20a. Mean ice thickness in the arctic sub-seas has different tendencies. It has an increasing trend in the Barents-Kara Sea and Baffin Bay-Labrador Sea, and decreasing in the others. The characteristic time scale of 7-10a wherein the river discharges leads the Fram Strait ice volume export is about the period that river water takes to be conveyed across the Arctic Ocean.(6) Using the simulated ice distribution in the Arctic Ocean and China precipitation, air temperature and SST in tropical key regions, the climate teleconnection were studied. Result showed: When the mean sea ice thickness is large in the central Arctic Ocean and Chukchi-Beaufort Sea , and small in the Barents-Kara Sea and Baffin Bay-Labrador Sea , the precipitation in South China, Tibetan Plateau, and the north part of Northeastern China are always smaller than normal, and v. v. When the mean ice thickness is small in CA, BC, East Siberian Sea and Greenland-Iceland-Norwegian Sea , and large in BL, The air temperature in north-eastern China, the southern of Tibetan Plateau, and Hainan Island, are always lower than normal, and v. v. In addition, when the sea ice is thick in BC and BL, the SST is larger in the middle and eastern Pacific Ocean, and is smaller in the tropical Southeastern Indian Ocean.

由于BOM没有考虑透射太阳辐射的物理过程，研究表明透射太阳辐射对北冰洋的能量收支起到重要作用，因此在BOM模式中引入了对透射太阳辐射的处理；通过对不同水道反照率的数值试验表明海冰厚度对水道反照率的敏感性不强，可能与海冰区水道面积占的比率很小有关；而模式模拟的北极海冰夏季&过度融化&主要源于NCEP再分析资料提供了偏大的太阳短波辐射；对北极海冰的气候变率进行了模拟研究，结果表明：模拟的海冰厚度变化与潜艇探测的海冰吃深度变化具有一致性；模拟和观测的亚欧大陆沿海的年内最大海冰厚度有很好的相关；模拟的海冰移速与长期平均的卫星反演的海冰移速具有相同的速度分布特征；模拟的各个海区海冰面积的变化趋势与卫星反演资料分析的结果基本一致；模拟与观测的主要海洋分区的海冰密集度具有很好的相关：弗瑞姆海峡的海冰体积和面积的输送涉及到海冰密集度、厚度和移动速度，是判断模式模拟能力的一个综合的指标，模式模拟的结果与卫星反演或重建的面积输送、体积输送具有很好的一致性；(5)分析了模拟的北极海冰厚度的气候变率及气候平均表层海流场，结果表明：北极海冰的总体积有显著减少的趋势，北极海冰总体积的变化具有10a际尺度振荡的特点，存在18-20a和12-13a两个主周期；北极海冰的平均厚度在各个海区的变化趋势不同，在巴伦支—喀拉海和巴芬湾—拉布拉多海地区海冰厚度有显著的增加趋势，而其它海区存在减少的趋势；通过对模拟的气候平均表层海流的分析表明，北极河流流量超前弗瑞姆海峡海冰流量7-10年的特征时间尺度与表层海流的气候分布存在着必然联系：(6)利用模拟结果以及中国降水、气温和热带关键区SST资料，讨论了北极各海区海冰平均厚度与中国降水、气温以及热带关键区SST的关系，结果表明：在北极中心海区和楚科奇—波弗特海海冰厚度偏大，在巴伦支—喀拉海以及巴芬湾—拉布拉多海海冰厚度偏小，则中国降水在华南地区、青藏高原和东北北部降水偏少，反之相反；在北极中心海区、东西伯利亚海、楚科奇—波弗特海以及格陵兰海海冰厚度偏小，在巴芬湾—拉布拉多海海冰厚度偏大，则在中国东北地区、高原南部地区和海南岛附近气温偏低，反之相反；另外，北极楚科奇—波弗特海和巴芬湾—拉布拉多海海冰厚度偏大时，在热带中东太平洋海温偏高，而在热带东南印度洋海温偏低。

In order to determine the immunomodulatory effect of the dietary intake of levamisole in the Chinese mitten crab, the crab were fed diets containing 0, 100, 200 and 300 mg levamisole kg1 dry diet for 7 days. The total haemocyte count, differential haemocyte count, phagocytic activity, respiratory burst, phenoloxidase activity and lysozyme activity were examined at 0, 2, 4, 6, and 8 weeks after administration of levamisole.

中文摘要：摘要：为测定在饲料中添加左旋咪唑对中华绒螯蟹的免疫调节作用，以0、100、200和300 mg · kg1干饲料的剂量将LMS添加于基础饲料中制成颗粒饲料，投喂中华绒螯蟹7 d，然后投喂不含LMS的对照组饲料。

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

In this study, by applying technology of plant physiology and biochemistry, combined with proteomics technology we analyzed effect of the low temperature stress on the physiological and biochemical responses on 4 week-old buffalograss treated at 5℃for 6 h and 24 h, and carried out the analysis of differential proteomics on leaf total protein under 5℃for 24 h. The aim of this study is to explore the mechanism of cold resistance on buffalograss.

本研究利用植物生理生化的研究手段，并结合蛋白质组学研究技术(双向电泳技术、质谱技术)，分析了在5℃低温胁迫6 h和24 h的条件下，野牛草生理生化的响应机制；对低温胁迫24 h的野牛草幼苗叶片总蛋白进行了差异蛋白质组的比较分析，以探索野牛草低温胁迫的抗性机制。

High quality total RNA were isolated from adult chicken's liver organ of four chicken breeds: silk fowl , normal plumed black-bone chicken , SuQin 96 layer chicken and Abar acer meat chicken . The differential expressed genes in liver organ of above chicken breeds were studied via DDRT-PCR technique for the first time.

本试验成功地从丝羽乌骨鸡、常羽乌骨鸡、苏禽96型蛋鸡以及爱拔益加肉鸡的成年鸡肝脏组织中提取到质量较高的总RNA，首次采用DDRT-PCR技术对四个家鸡品种肝脏中差异表达的基因进行了研究。

Total RNA were extracted from the gonads of two types of Tilapias.The cDNA copies of differential expressed mRNA species were amplified by DD-PCR using an anchored primer 5' dTuN, combined with two of arbitrary' primers and one long primer, respectively.

本文从罗非鱼的性腺提取的总RNA为模板，用5'dT_11N锚定引物和两个随机引物以及一个长引物组合进行DDRT-PCR扩增。

In this study, we used poly L-latcide, poly DL-latcide-co-glycolide 75:25 (PLGA7525) and poly DL-latcide-co-glycolide 50:50 (PLGA5050) during citric acid inducing ammonium bicarbonate gas forming process to form porous polymer film, and rolled the porous polymer film to make nerve conduits with pores and multi-layered. Electron Spectroscopy for Chemical Analyzer and Attenuated Total Reflectance – Fourier-Transform Infrared Spectrometer were employed for determining elements' functionabilities and chemical compounds. Charge Coupled Device camera and Scanning Electron Microscope were employed for macroscopic and microscopic morphologies and structural observation. Differential Scanning Calorimetry was employed for measuring glass-transition temperature. Nano-indentation system was employed for measuring elastic modulus and hardness. Biodegradation and water absorption ratios were measured to analyze their chemical properties and SEM was employed for microscopic morphology of the tested nerve conduit.

本研究将以聚乳酸、聚乳酸-聚甘醇酸共聚合物（75：25）、聚乳酸-聚甘醇酸共聚合物（50：50）做为研究材料，以柠檬酸诱发碳酸氢铵发泡之气泡成型法制作具有相连通孔隙之高分子薄膜，再将高分子薄膜卷制成特殊多层的导管，制作具有多层孔隙之新型神经再生导管，以电子能谱化学分析及减弱式全反射-傅立叶转换红外线光谱分析进行成份分析及化学元素测定；以电荷耦合元件摄影机、扫瞄式电子显微镜进行多层孔隙型神经导管巨观及微观形态结构观察；以示差扫瞄热量量测分析玻璃转换温度、奈米三维量测弹性模数及硬度；以及进行降解性质测定，并以扫瞄式电子显微镜进行降解后之导管微观结构形态观察。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。