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The results showed as follows: 1 Extraction of gelsemium total alkaloids. Total alkaloids were extracted with 95 per cent ethanol. The extract rate was 0.519 per cent and the extract purity were 88.02 per cent and 87.38 per cent; Total alkaloids were extracted with chloroform .The extract rate was 0.416 per cent and the extract purity were 89.02 per cent and 94.52 per cent. 2 Isolation of gelsemium total alkaloids with TLC. Total alkaloids contained five or more alkaloids. The optimal developing agent was chloroform :methanol :ethyl acetate(9:l:0.3),the optimal eluent was methylene chloride:methanol(92:8). 3 Isolation of total alkaloids with column chromatography. Alkaloid A and alkaloid B were obtained, alkaloid B was identified as koumine.

研究结果如下:1、钩吻总碱的提取:用95%的乙醇提取钩吻生物碱提取率为0.519%,其提取物纯度为88.02%、87.35%;用氯仿提取钩吻生物碱提取率为0.416%,其提取物纯度为89.02%、94.52%。2、钩吻总碱的薄层层析分离:总碱中含有5种或5种已上的生物碱;氯仿:甲醇:乙酸乙酯(9:1:0.3)是最佳薄层层析的展开剂;二氯甲烷:甲醇(92:8)是柱层析的最佳洗脱剂。3、钩吻总碱的柱层析分离:共得到两种钩吻生物碱单体成分碱A和碱B,碱B为钩吻素子,碱A可能是钩吻素甲。4、钩吻总碱的急性毒性试验:钩吻总碱小鼠腹腔注射的半数致死量LD_(50是:7.38mg/kg,95%的可信限范围为:6.67mg/kg~7.94mg/kg。

Analysis results on townships and villages general statuses showed township health center and village clinic are the major health care facilities in rural poor areas. The number of staff in 36 THCs and 104 VCs were a little lower than the national average. In township health centers, 59. 9% staff are with primary or without any professional titles and 35. 9% of the total staff have no professional degrees. The average gross value of fixed assets per THC is 186000 yuan, among which special equipment values 41000 yuan. Among professional buildings 43. 3% of total area were dangerous. Only one THC can provide emergent service. 47. 2% THCs be able to diagnose and deal with common emergent stomachache. The provision of health services was lower than average level of national rural areas. Frontier Model analysis showed regression coefficients of total outpatient emergency service, total inpatients, average personnel income, number of total staff, number of doctors and technical index were all larger and positive. It meant that these variables would contribute more to the cost-frontier, and their increase would cause the total health cost rising. So increasing health service provision, improving service quality and functions of health facilities may be the best ways to increase efficiency of these facilities. Also the model showed THC inefficiency eu was 1. 68, which means 40% of the cost wasted. Apart from internal and external factors, residents'health care utilization deficiency and inequity is one of major factors causing inefficiency eu.

乡、村基本情况结果表明,农村贫困地区乡卫生院和村卫生室是农民就近就医的主要场所;36所乡卫生院以及104所村卫生室的人员数量,略低于全国同期农村平均水平;在乡卫生院中,初级职称和无职称者的比例占59.9%,无专业学历者占35.9%;平均每院固定资产总值18.6万元,其中专用设备4.1万元,业务用房中危房面积占43.3%;36所卫生院中,具备急救条件的只有1所,能诊断和处理常见急腹症的占47.2%;卫生服务提供量均低于全国农村平均水平;Frontier模型方法显示,总门诊人次、总出院人次、平均人员工资、人员总数、医生数和技术指数等指标的回归系数较大,且均为正数,即这些变量对"前沿"成本的贡献较大,其正向变化均会带来卫生服务总成本的增加,表明增加卫生服务提供量,提高质量以及完善机构服务功能,是提高乡卫生机构效率的有效途径;同时Frontier模型结果显示,卫生院的低效率〓为1.68,经换算即40%的成本处于浪费状态;影响乡卫生院低效率〓的因素有外部和内部的,而需方卫生服务不公平及利用不足是其主要因素之一。

The results indicated that the growth of 3-year-old Prunus salicina was significantly higher than that of the other species, such as 139.1% and 153.7% higher than Toona sinensis and Juglans regia in height growth, respectively. The maximum percentage of DBH more than 1.0 cm was 91.9% for P. salicina. Next were 49.5% and 48.8% for Ulmus pumila and Pteroceltis tatarinowii, respectively. The contents of total N, total P and total K ranged from 1.36% to 2.52% in the leaves of testing species. The highest content of total N and total P was found in the leaves of Catalpa bungei, while the highest content of total K was observed in P. saltcina leaves. The results indicated that P. salicina, P. tatarinowii, U. pumila had well adaptability on the Karst area both in the growth status and the improvement to the soil with the fallen leaf.

结果表明:(1)3年生冰脆李的生长量明显优于其他树种,其高生长比香椿、核桃分别大139.1%和153.7%;植株胸径大于等于1.0 cm株数的百分比以冰脆李最高达到91.9%,其次是榆树和青檀,分别是49.5%和48.8%;(2)8个树种落叶的全N、全P和全K的总含量变动为1.36%~2.52%,其中全N、全P含量以滇楸最高,全K含量以冰脆李最大;(3)从各树种落叶前后各元素含量及其转移情况看,冰脆李、青檀表现出对大部分营养元素具有较高的循环利用养分或保持养分的能力,而香椿及刺槐相对较弱。

Sediments of this system were sequentially extracted in natural granularity. The result showed that there was a good linear relationship between organic matter and org / bact P contents in sediments. This organic matter in sediments was the source of org / bact P. The total phosphorus in sediments dominated the content of total dissolved phosphorus and phosphate in interstitial water. In addition, there were good relatiohships between total phosphorus in sediment and the total dissolved phosphorus in interstitial water, and between total phosphorus in sediment and phosphate in interstitial water. FeP and AlP in sediments were correlative with Eh and phosphate in interstitial water, respectively. This showed that the FeP and AlP contents were affected by Eh in interstitial water and the total content of FeP and AlP dominated the content of phosphate in interstitial water.

对沉积物—间隙水中磷形态的连续提取分析发现,自然粒度下,沉积物中有机质含量与沉积物中有机/细菌聚合磷存在很好的线性相关性,说明沉积物中有机质是有机/细菌聚合磷的源;沉积物中总磷决定间隙水中总溶解性磷和溶解性磷酸盐的含量,并且总磷与间隙水中总溶解性磷和溶解性磷酸盐存在很好的相关性;沉积物中FeP和AlP分别与间隙水中的Eh和溶解性磷酸盐存在相关性,说明Eh的大小严重影响FeP和AlP的含量,但是FeP和AlP含量之和又主导着间隙水中溶解性磷酸盐的含量。

The total differential cross section, elastic differential cross section and inelastic differential cross section for collision between He isotopic atoms and NO molecule have been calculated by using close-coupling approximation method.

汪荣凯 贵州师范大学理学院,贵阳 550001沈光先贵州师范大学理学院,贵阳 550001;四川大学原子与分子物理研究所,成都 610065宋晓书贵州师范大学理学院,贵阳 550001;四川大学原子与分子物理研究所,成都 610065

The results showed that about610 protein spots could be visualized on the two dimensional electrophoresis map bycoomassie brilliant blue staining within Mr 9.0~100.0kD and pH 4~7,and about 94 spotswere differential expressed between male-sterile line and its maintainer line by PDQuestsoftware.Among those differential proteins,about 49 spots and 40 spots were differentialexpressed between uninucleate anther stage and binucleate stage respectively;Meanwhilecomparing with the 2-DE maps of the total proteins from the binucleate anther stage anduninucleate stage,15 differential spots were detectable at male-sterile line;and 16 differentialspots were detectable at its maintainer line.

在分子量9.0~100.0 kD、等电点4~7线性范围内,可识别约610个蛋白质点,PDQuest软件分析结果表明:在单核期不育系和保持系之间存在40个差异蛋白质点,二核期两者共有49个差异点;不育系在单核期和二核期两者之间存在有15个差异点,保持系在两个不同时期有16个差异点;不育系与保持系间在表达量上的差异蛋白质点以及不育系与保持系间特异表达或特异缺失的蛋白质点很可能与小麦雄性不育有关。3。

By the FEA model that the recharge effect had been taken into account, the surface settlement and the surface differential settlement of the ground surface which was out of the recharge position could be greatly reduced, the recharge effect at far foundation pit wasmuch more apparent than at the near position. By the FEA model that the water-seal effect had been taken into account, the surface settlement and surface differential settlement of the ground surface could be largely reduced; the reduction of the total settlement and the differential settlement increased as the length of water-seal wall grew.

考虑回灌作用的有限元模型计算分析表明:设置回灌井或者回灌排水沟都可减小回灌处以外的地表总的沉降和不均匀沉降,随着排水沟离开基坑边距离的增加,回灌点以外的总沉降量和不均匀沉降都减少,回灌井埋设深度对周围地表沉降影响较小;通过考虑止水帷幕的有限元模型计算分析表明:设置止水帷幕可大大减小基坑周围土体总沉降和不均匀沉降,随着打设止水帷幕深度的增加,基坑周围地表总沉降和不均匀沉降都减小。

It is shown from the investigation results that the total consumer's surplus for the Qingdao First Bathing Beach is RMB 197 million yuan/year, the total willingness to pay for beach usage is RMB 6.31 million yuan/year, the total willingness to donate for beach environmental protection is RMB 174 million yuan/year, so the total beach use value has exceeded RMB 430 million yuan/year. If the nonuse value for the Qingdao First Bathing Beach is included, the total beach value is quite considerable.

调查结果发现,青岛市第一海水浴场消费者总剩余达到1.97亿元/年,海滩年度总使用支付意愿达到6310万元,环境保护捐赠意愿达到1.74亿元,总海滩使用价值超过4.3亿元,再加上非使用价值,其价值总量相当可观。

The compound pollution sign exists in the soil between total cadmium, total hydrargyrum, total plumbum and chrome. The prominent correlation exists in total cadmium and total plumbum. The correlation coefficient is very lower between heavy metals in the vegetable body. The correlation coefficient is very prominent between cadmium and plumbum in the vegetable body. The correlation between vegetable and soils heavy metal content of the cadmium is very prominent and others is very lower.

土壤中全镉、全汞、全铅和全铬这四种重金属之间存在着复合污染的迹象,其中全镉和全铅存在极显著的相关性;植物体内重金属之间的相关系数很低,只有镉和铅具有显著相关性;植物和土壤重金属含量的相关关系只有镉的含量呈显著关系,其它相关性较低。

It also revealed the antagonistic effect on myocardial hypertrophy pronouncedly in total timosaponins and total astragalus saponins treated group. Conclusions Total timosaponins has the restrain effect on myocardial hypertrophy induced by isoprenaline in mouse. Among the selected doses, the theoretical optimal proportion of total timosaponins and total astragalus saponins is (25+100) mgkg^(-1). The complex prescription can enhance the action.

黄芪总皂苷对盐酸异丙肾上腺素所致小鼠心肌肥厚具有显著的抑制作用;知母总皂苷与黄芪总皂苷在所选择剂量范围内的理论最佳配比为(25+100)mgkg^(-1);知母总皂苷与黄芪总皂苷组成的复方有抗盐酸异丙肾上腺素所致小鼠心力衰竭作用。

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