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The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P>0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P<0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P<0.05),while the difference between control group and blank group was not significantP>0.05The difference of alkaline phosphatase activities among three groups was not significant(P>0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP<0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P<0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P>0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后,其各时间点CCK-8法检测值与空白对照无显著差异(P>0.05),成骨细胞均在第5天达到增殖高峰期;培养7天后,实验组细胞蛋白含量高于对照组及空白组(P<0.05),后两者之间则无显著差异P>0.05碱性磷酸酶活性在三组间均无显著差异(P>0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解,第4周时更为明显,纳米珍珠层粉较之微米珍珠层粉降解更快,而空白对照组骨洞阴影仍可见,至8周时,则所有组骨洞均己闭合修复,X-ray下已不可见原钻孔痕迹,恢复正常骨质密度;硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快,空白组速度最慢P<0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快;由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好,无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快,至植入术后24周,实验组骨缺损区接近正常骨密度,对照组骨缺损区密度较低,空白组则呈现骨不连状态;骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P<0.05,24周实验组的骨密度值与术前所测得的正常值无显著性差异P>0.05动物取材大体所见均显示组织相容性良好,骨组织逐渐由植入材料两端向中央生长;常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组,至术后24周,实验组骨髓腔与材料已呈相交通状;硬组织磨片荧光显微镜下观察,两组材料在术后8周处于骨生长最快速时期,16周时速度开始减慢,术后4、8、16周时实验组的新骨生长速度均较对照组的快

The energy loss through feces was 0.077C (C: food consumption, KJ/d) and the energy loss through excretion was 0.052C at maximum feeding rate. In that the ratios of F and U to C were low in fish energy budget equation (Cui et al., 1997), the energy loss through feces at optimum feeding rate could be estimated by 0.077C〓(C〓: the optimum feeding rate) and the energy loss through excretion at optimum feeding rate could be estimated by 0.052C〓. The relationship between total energy content for immature gibel carp and body weight was lnE=1. 476+1. 127lnW.

最大摄食率条件下,异育银鲫排粪能损失:F=0.077C,排泄能损失:U=0.052C;由于排粪能及排泄能在能量收支方程中所占比例不大(崔奕波等,1997),最适摄食率条件下的排粪能损失可估计为F〓=0.077C〓(C〓:最适摄食能,KJ/d),排泄能损失可估计为U〓=0.052C〓;性未成熟异育银鲫的能量含量与体重的关系为:lnE=1.476+1.127lnW

The content of hexadecanoic acid (C16: 0) and oleic acid (C18: 1) in the ovary and the fat body was about 80% of the total fatty acids.

卵巢和脂肪体中C16∶0和C18∶1的含量约占脂肪酸总量的80%左右。

Therefore can pass to use the cupric sulfate sulfuric acid potassium for the catalyst take sulfuric acid as to carry the body to digest it, make the amino acids chelate match the conversion for dissolve sex easily of ammonium sulfate and white vitriol, then absorb a fixed amount to try the kind to join 40% sodium hydroside combine under the steam heat function make ammonia the spirit swims to leave out, through cool off to respond the born boric acid with boric acid, be got the total nitrogen content by the standard aqua titration of hydrochloric acid immediately after;But the white vitriol can immediately join the hydrochloric acid hydroxylate , fluoride to turn the in order to cover up, carry on the titration but get the content of its zinc with the EDTA mark liquid..

因此可以通过用硫酸铜硫酸钾为催化剂以硫酸为载体将其消化,使氨基酸螯合锌转化为易溶性的硫酸铵和硫酸锌,然后吸取一定量的试样加入40 %的氢氧化钠并在水蒸气高温作用下使氨气游离出来,经冷却与硼酸反应生成硼酸铵,接着由盐酸标准溶液滴定得到总氮含量;而硫酸锌即可加入盐酸羟铵、氟化氢铵为掩蔽剂,用EDTA标液进行滴定而得到其锌的含量。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板,用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF,其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体,构建的重组质粒命名为pET-30a-PN;将pET-30a-PN转化到大肠杆菌BL21 (DF3)中,在IPTG诱导下进行表达;SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白,重组蛋白以包涵体形式存在;薄层扫描结果表明表达产物占菌体总蛋白的30.5%;Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应,说明该重组蛋白具有免疫学活性。

In the Stage III(6st ~10th days) the total length of the leptocephali stayed unchanged, the fish body is not transparent any more, organs and systems grew continuously.

StageⅢ的体长并无明显增加,组织及器官发育逐渐接近完全。

During the motion of total knee joint, the tibiofemoral interface bears loading several times larger than its body weight. In normal functions, the applied forces are uniformly distributed on the polyethylene cushion at the tibiofemoral cavity. Nevertheless, joint stability resulting from the support of surrounding tissues such as tendonous muscles and ligamentous systems, is incapable to withstand as normal.

人工膝关节在活动时,脛骨-股骨关节界面受到数倍於体重的负载;在正常功能下,其受作用力均匀分佈在脛骨-股骨之间的聚乙烯的垫片上,然而,原有来自於筋-肌肉与韧带系统对人工股骨远端与脛骨的稳定作用则降低。

Dual x-ray absorptiometry was used to measure BMD of the lumbar spine, femoral neck, total hip, and nondominant one third radius, along with body mass index.

使用双能X射线吸收仪评估腰椎、股颈、髋骨、非惯用三分之一桡骨的骨质密度、还有身体质量指数。

DST showed that the pretreatment with dexamethasone can not repress the total number of CRF immunoreactive neurons in the rat model induced by CUMS, by contrast with a normal repression in the number of CRF neurons in rat induced by acute stress. In brief, the rat model induced by CUMS represented decreased body weight, obvious depression-like behavior, hyperactivity in HPA axis and nonsuppression on the DST, which fits the criteria and neurobiological feature of depression.

简而言之,CUMS诱导的大鼠模型有典型的抑郁症样行为和表现、HPA轴活性升高、DST的失抑制,符合抑郁症模型的行为学标准和主要神经生物学特征,是抑郁症稳定可靠的模型,也是研究应激诱导的糖皮质激素升高所带来的神经生物学效应的良好模型。

Umbrella form inflorescence normally 2~4 bunch gives birth to short branch of Yu Xie armpit, short branch grows 1~2 millimeter; Inflorescence total stalk grows 5~10 millimeter, by short fluff; Bud piece 4, wide egg form, have 5~6 arteries and veins, outside a few without wool, inside face by white short fluff; Inflorescence of each umbrella form has beautiful 4~6, the leaf is opened or open at the same time with the leaf first; Peduncular grow about 1.5 millimeter, close by white fluff; Perianth piece 6, elliptic, two sides by short fluff; In male flower can Yo is staminal 9, filament has grey yellow fluff, form of heart of shape of aegis of body of gland of ministry of the 3rd round of stamen radical, do not have a handle nearly, degrade pistil is not had; Stamen degrades in female flower 9, staff state, by grey yellow fluff, ovarian crock form, style is dumpy, post head shape, shallow crack.

伞形花序通常2~4个簇生于叶腋短枝上,短枝长1~2毫米;花序总梗长5~10毫米,被短柔毛;苞片4,宽卵形,有5~6条脉,外面几无毛,内面被白色短柔毛;每一伞形花序有花4~6朵,先叶开放或与叶同时开放;花梗长约1.5毫米,密被白色柔毛;花被片6,椭圆形,两面被短柔毛;雄花中能育雄蕊9,花丝有灰黄色柔毛,第三轮雄蕊基部腺体盾状心形,近无柄,退化雌蕊无;雌花中退化雄蕊9,棍棒状,被灰黄色柔毛,子房壶形,花柱粗短,柱头头状,具浅裂。

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In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国,慢性酒精中毒,肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题,你可以随时联系我。

Very pretty, but the airport looks more fascinating The other party wisecracked.

很漂亮,不过停机坪更迷人。那人俏皮地答道。