查询词典 topo

METHODS: MyoD cDNA fragments were extracted from plasmids pEMSV-MyoD with polymerase chain reaction, and PCR was used to clone the whole-length gene of MyoD. After adding CACC sequence at 5' end, MyoD gene was cloned by orient topology into transfer ventor, pENTR/D-TOPO. Objective gene was transferred into adenoviral expression vector DNA via pENTR/D-TOPO vector. The recombinant adenoviral vectors transfected into HEK293A cells by using lipofectamine were packaged and amplified.

从pEMSV-MyoD质粒上用聚合酶链反应法扩增出MyoD cDNA片段，再通过聚合酶链反应使MyoD基因加上CACC序列接头，经过定向拓扑克隆使目的基因连接到转移载体上，再通过LR酶促反应，将目的基因转移到腺病毒表达载体DNA上，获得MyoD基因重组的腺病毒DNA，用脂质体转染法转染HEK293A细胞，包装扩增出MyoD基因重组的腺病毒。

Methods: 60 histologically verified HCC and 42 non-HCC specimens were examined for Topo-Ⅱ by immunohistochemical methods.

应用免疫组织化学S-P法检测60例原发性肝细胞瘤及42例非肝癌肝组织中Topo-Ⅱ的表达。

Results (1) The positive expression rates of Pgp, GSTπ and TopoⅡ in 35 cases with HCC were 85.7%, 71.4% and 31.4% respectively.

结果 ①35例肝癌中的Pgp、GSTπ、TopoⅡ的阳性表达率分别为85.7%、71.4%、31.4%。

Result The positive rate of P gp, HSP 27 and TOPOⅡ was 49 69%; 57 62% and 47 85%, respectively, in digestive tact cancer...

结果P gp在消化道癌中的阳性率为 4 9 6 9%，HSP2 7在消化道癌中的阳性率为 5 7 6 7%，TopoⅡ阳性率为 4 7 85 %。

During one battle, El Topo is wounded.

在一次战斗中，El Topo受了伤。

He positive rates of TopoⅡα and MGMT in limphmetastasis group were higher than that in nonlimphmetastasis group.

opoⅡα和MGMT有低度相关性（γ=0234），61岁以上老年组TopoⅡα和MGMT阳性率均低于60岁以下组，有淋巴结转移组阳性率高于无转移组。

Results: The positive rates of MGMT and TopoⅡα expressions were 5921%(45/76) and 6842%(52/76) respectively.

结果： 乳腺癌中MGMT阳性率为5921%（45/76），TopoⅡα阳性率为6842%（52/76）。

A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物，自接种H5N1亚型病毒的鸡胚组织中提取RNA，反转录后采用高可信度DNA聚合酶经PCR扩增M1基因，采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达，纯化获得N末端携带多聚组氨酸标签的重组蛋白，分子量约29.8 kDa。

JPYFHY Recipe can inhibite the tumor of lung cancer transplanted animals, and the JPYFHY Recipe drug-serum can block the prolife ration of A549/DDP in vitro by inducing cancer cell apoptoses and boking cell cycle; It can enhance the chemotherapy synergism effects when they are used in combination and reverse multidrug resistance which because of DDP. The mechanism of these reversing drug resistance maybe is related to downregulating the expression of MRP and GST and upregulating the expression of TopoⅡ.

健脾益肺化瘀方对动物移植性肺癌具有抑瘤作用，能阻滞肺癌细胞进入增殖期，从而达到抑制肺癌细胞生长的目的；并能增强DDP的敏感性和具有对DDP的耐药逆转作用，其作用机理与健脾益肺化瘀方抑制A549／DDP耐药基因MRP和GST的表达和增强TopoⅡ活性密切相关。

We synchronized the wheat cell suspension cultures, then added Novobiocin—an inhibitor of TopoⅡ to the cell suspension, changs of the ratio of M/T were observed within 48 hours.

用aphidicolin使小麦悬浮系细胞同步化，然后向细胞培养液中加入TopoⅡ抑制剂新生霉素，48小时内观察分裂中期细胞比率的动态变化。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失