查询词典 topo

The invention discloses an InP quantum point preparing method, comprising the steps of:(1) mixing InCl3 with trioctylphosphine oxide, and preserving heat at 90-110DEG C to prepare a solution whose In content is 0.1-0.3mol/L;(2) raising temperature to 130-180DEG C, and charging argon gas;(3) injecting PSi(CH333 into InCl3-TOPO compound in the molar ratio of 1 to 1-1 to 2;(4) when the solution color turns into transparent red or orange, raising temperature to 260-270DEG C and preserving heat;(5) lowering temperature to 90-110DEG C and injecting dodecyl amine, decyl amine or mercaptan;(6) dissolving reacting mixture in nonpolar solvent to form transparent colloidal solution, then adding in polar solution until the colloidal solution is muddy, and centrifugally separating and obtaining deposits and supernatant; and (7) centrifugally separating the supernatant, where the above steps (1)-(5) are performed.

本发明公开了一种InP量子点的制备方法，其步骤为：①将InCl 3 与三辛基氧膦混合，在90～110℃保温制得In含量为0.1～0.3mol/L的溶液；②升温到130～180℃，通入氩气；③将PSi(CH 3 3 3 注射入InCl 3 -TOPO复合物中，其摩尔比为1∶1～1∶2；④待溶液颜色变为透明的红色或橙红色，将升温260～270℃保温；⑤降温到90～110℃，注入十二胺、癸胺或硫醇；⑥将反应混合物溶于非极性溶剂，形成透明的胶体溶液，然后加入极性溶剂，直至胶体溶液出现混浊状，然后离心分离获得沉积物和上层清液；⑦取上层清液进行离心分离。上述步骤①～⑤在保护气氛下进行。

In addition, the r8 cDNA was also expressed at high level in pBAD/TOPO system and showed esterase activity after enterokinase cleavage followed by purification and renaturation.

此外类凝血酶基因r8在pBAD／TOPO中也得到高效融合表达，表达产物经酶切、纯化和复性，测得精氨酸酯酶活性。

It is the story of El Topo, a gunslinger who sets out for revenge against the outlaws who slew his wife.

它讲了一个枪手El Topo为被杀害的妻子报仇的故事。

The 7.4 kb DNA fragment containing the S. Choleraesuis std fimbrial gene cluster was PCR amplified and cloned into the pCR-XL-TOPO vector. The open reading frames of the std fimbrial gene cluster were analyzed. The resulting recombinant DNA was transformed into Escherichia coli HB101 strain.

本实验室藉由PCR方式，增幅7.4 kb的std线毛基因组，将其选殖至pCR-XL-TOPO质体DNA，委外定序后，分析std线毛基因组之开放性读框，进而将此重组DNA送入大肠杆菌HB101，并藉由RT-PCR及西方点墨试验观察到S。

Results 1. The expression of P53, KI-67, hTERT and ToPo II a protein was higher in astrocytoma than that in nomal brain tissue, the expression of these markers was obversely correlated to the grade of astrocytoma (P.05). The expression of Fas was higher in astrocytoma than that in nomal brain tissue, on the contrary, expression of P16 was lower in astrocytoma than that in nomal brain tissue.

结果 1、星形细胞瘤中P53、KI-67、hTERT与ToPoⅡα蛋白表达阳性率高于正常脑组织，P53、KI-67、hTERT与ToPoⅡα蛋白表达与星形细胞瘤的病理组织学分级呈显著正相关(P.05)；Fas蛋白在星形细胞瘤中的表达高于正常脑组织，而P16蛋白表达低于正常脑组织，Fas、P16蛋白表达与组织学分级无显著相关性。

Previous studies have shown that the mechanisms of MDR involve Pgp, MRP, LRP, BCRP, GSH/GST, PKC, Topo DNA plerosis, genes related to apoptosis and changes of cellular environment (such as pH, hypoxia and temperature).

目前发现的肿瘤多药耐药机制主要涉及Pgp、MRP、LRP、BCRP、GSH/GST、PKC、TopoⅡ、DNA修复、多种凋亡相关基因和肿瘤细胞生活的内外环境变化。

Previous studies have shown that the mechanisms of MDR involve Pgp,MRP,LRP,BCRP,GSH/GST,PKC,Topo DNA plerosis,genes related to apoptosis andchanges of cellular environment (such as pH,hypoxia and temperature).

目前发现的肿瘤多药耐药机制主要涉及Pgp、MRP、LRP、BCRP、GSH/GST、PKC、TopoⅡ、DNA修复、多种凋亡相关基因和肿瘤细胞生活的内外环境变化。

Objective: To evaluate the expression of Topo-Ⅱ in hepatocellular carcinomaan and to explore the relationship between the expression of Topo-Ⅱ and biological behavior of HCC.

目的：探讨在原发性肝细胞癌中Topo-Ⅱ的表达以及与原发性肝细胞癌生物学特征的相互关系。

Use pCR2.1-TOPO and pCR2.1-Blunt II TOPO vectors as models, we investigated the possibility to use TOPO T/A vector (pCR2.1-TOPO) to clone blunt-ended PCR product and to use TOPO blunt vector (PCR-Blunt II TOPO) to clone 3'-A PCR product.

以pCR2.1-TOPO与pCR2.1-Blunt II TOPO载体为模型，我们研究了利用TOPO T/A载体（pCR2.1-TOPO）克隆平端PCR产物与利用TOPO 平端载体（pCR2.1-Blunt II）克隆3'-A末端PCR产物的可能性。

In the second part, we used tri-octyl phosphine oxide as a model of TRPO,to study the γ radiolytic behavior of TOPO and its CH solution and the emulsificationphenomenon in the separation processes of radiolytic products. The structures of finalproducts were analyzed with IR spectra, UV spectra, MS and elemental analysis.

本论文的第二部分，以TOPO为TRPO的模式化合物，用终产物分析的方法对TOPO的辐解特性及乳化过程进行了研究，并运用红外光谱、紫外光谱以及元素分析、质谱等方法对乳化物的结构进行了分析。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失