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The regulation of gene expression is to have relations to the assembly of the DNA binding proteins which bind to DNA to initiate the transcription. Some of these proteins have the ability to promote bending or looping of DNA. The FIS protein regulates gene expression in Escherichia coli by this property of bending DNA. There are many researches to discover the mechanism of FIS protein binds to DNA. However the experiment data only show the supposititious results and figure out the model of FIS-DNA complex structure. This research uses molecular dynamics simulation to compute the detail of the interaction between FIS and DNA. We observed FIS prominently affects the DNA bending by the type of local bending. Analyzing the DNA structure properties, roll and slide, also demonstrates the local bending type and region. The hydrogen bonds between FIS and DNA are analyzed to compare with the experiment data. Finally we compute the binding free energy between FIS and DNA to estimate the result of simulation and compare with the value of λ-repressor-DNA complex.

基因的表现与结合於DNA促使DNA转录作用起始的DNA结合蛋白组装有关,一些这类的蛋白质具有促使DNA扭曲或将DNA形成环状的能力,FIS蛋白质即是藉由扭曲DNA的特性调控大肠杆菌基因的表现,目前已有很多的研究发现FIS与DNA结合的机转,然而这些实验的数据仅能显示推测性的结果,进而利用这些结果推论出FIS与DNA聚合体结构的模型,本研究利用分子动态模拟的方式计算FIS与DNA交互作用的细节,我们观察到FIS主要影响DNA的扭曲方式为DNA区域扭曲的类型,并且分析DNA结构特性roll与slide也指出扭曲方式为区域扭曲与其扭曲的区域,我们也分析FIS与DNA之间的氢键,并且与实验的资料作比较,最后我们计算FIS与DNA之间的结合自由能评估模拟的结果,并且计算出的值和λ-repressor与DNA聚合体结构的实验值作比较。

Because no matter how offensive you are not the BOSS fight, and ordinary weapons do not break playing guitar community (clearly on the handsome sword in hand, do not get how to use it, anyway, a handsome young man had become unconscious), was down after the Education Secretary, told him to take the female lead, a result, the female lead along with everyone in order to save the screen to return to village homes, while we worry about when the old man down the hill has come, and the organization of the armed forces ready to defend villages, leaving the protagonist told to look at the guy next door, and the handsome guy dialogue, he knows that he is wrong, and the last a sword to the hero (only this can break the sword knot profession, if you can not find love him, but I can not guarantee that will not bully your BOSS), came to the coast, is the iron man was waiting for the protagonist, exchanged a few words, a small island就往advance, when the one to the island, iron man down due to injury a, the protagonist of a person can only move forward, and entered after hearing the voices are very familiar with is the Sister, no, it is a bandage women!!!

因为这里不管你怎么攻击,都是打不到这个BOSS的,普通武器是打不破他的结界(剑明明就在帅哥手上,怎么不抢来用呢,反正帅哥已经昏迷了),被打倒后,司教要女主角跟他走,于是,女主角为了救大家就跟着去了,画面回到村长家,正当大家担忧时,山上的老头下山来了,并组织军队准备保卫村庄,临走时告诉主角,去隔壁看看帅哥,和帅哥对话,他知道自己错了,并把最后一把剑交给了主角(只有这把剑能破结界,要是你喜欢可以不去找他,不过我不保证BOSS不会欺负你),来到海边,居然是铁拳男等着主角,寒暄两句,就往小岛前进,当一到岛上,铁拳男就因旧伤复发倒下了,主角只能一个人往前走了,进入后就听见很熟悉的声音,是修女,不对,是绷带女!!!

In the part of empirical analysis of Chinese IPO first-day return, the difference between existing study and this study is that we use principal components analysis to extract from five factors to construct investors'sentiment index, add it to controled varibles, on which we examine the influence of offering mechanism on IPO first-day return. Moreover, we gather statistics and compare the IPO holding return for lockup period between fixed-price-class offering method and bookbuilding-class offering method, as well as price range between Chinese A-share market and Hongkong stock market, which prvide comprehensive evidence to appraise Chinese IPO bookbuilding mechanism. 2. Compare to the existing literatures which consider discount or allocation, incentive allocation and discount are considered at the same time in optimal mechanism design in this dissertation, and the offering bottom price is introduced to the price range. We investigate the relationship between price range and allocation quantity, and obtain the optimal price range and allocation strategy so that the advantages of independent allocation are reflected. On the other hand, the disadvantages of independent allocation are reflected in the manipulation in case of no restriction on IPO allocation. To distinguish from the existing literatures, bookbuilding mechanism is introduced to the model. We study the allocation strategy adopted by the underwriter in the pooling equilibrium of manipulation. In addition, we analyze the incentive compatibility conditions to which the existence of pooling equilibrium should satisfy and examine some factors'impact on the existence of manipulation.3. In bookbuilding without independent IPO allocation, Chinese realistic IPO background is considered and the condition of bookbuilding and fixed-price hybrid offering is added. Divide bookbuilding and fixed-price hybrid offering into sequential hybrid and simultaneous hybrid, and point out that the key points of subscription strategy in the two kinds of hybrid are different. Through modeling and simulation we obtain the impact of institutional investors'subscription strategy on bookbuilding without independent IPO allocation, which fill the gap in related research. Moreover, in the part of IPO bookbuilding with over-allotment option, the pricing strategy is divided into hot-IPO strategy and weak-IPO strategy. Take into account the procedure of Chinese IPO with over-allotment option, it presents how the underwriter determines the pricing strategy and what effect it brings to offering price and issue size through modeling and simulation. Therefore, it is instructive as a complement to existing literatures.

此外,对我国A股市场询价发行方式与固定价格发行方式下的IPO锁定期到期时持有收益率、以及我国A股市场与香港市场的询价发行价格区间进行了统计比较,为评价我国IPO市场的询价发行提供了比较全面的依据。2、相比已有文献仅考虑折价和分配中的一种激励措施,本论文在有自主配售权下的机制设计中并用了分配与折价两种激励措施,并且在价格区间的制定中引入发行底价,考察了价格区间与分配量之间的关系,得到最优的价格区间和分配策略,从中体现自主分配权的优点;在有自主分配权下的操纵行为研究中则体现了自主配售权的弊端,与已有文献不同的是,在模型中引入了累计投标询价机制,研究了承销商在混同均衡操纵中采取的分配策略,并对操纵存在的条件进行分析讨论,得出各个因素对混同均衡操纵存在的影响。3、在无自主分配权下的询价发行中,充分考虑了我国实际应用的背景,加入了与固定价格发售混合的条件,并将询价与固定价格混合发行分为序贯与同步两种方式,指出机构投资者在两种情况下申购策略的侧重点各有不同,通过对序贯混合发行方式下的网下累计投标策略以及同步混合发行方式下的资金分配策略的模型建立和数值仿真,考察了机构投资者申购策略对无自主分配权下的询价发行的影响,填补了相关研究文献的空白;在包含超额配售选择权的询价发行中,将发行定价策略分为热销策略和弱销策略,在考虑了我国含超额配售选择权的发行流程下,通过建模以及数值仿真得出承销商会对发行定价策略作出怎样的选取,进而会对新股发行价格、发售数量产生怎样的影响,对目前相关研究的缺乏做出了有益的补充。

The research work of this paper is divided into five parts: The first ,according to the work characteristics of ship's auxiliary boiler, this paper is to design applied project in the automatic control system of ship's auxiliary boiler; The second, according to the work request of the cargo winch of alternating three-speed change-pole speed-adjusting, this paper is to determine adopting the method of the PLC control; The third, this paper is to design PLC application in three -phase induction motor control, to give the ladder chart, program and input and output circuit diagram; The fourth, this paper is to research the method of the ship's combined starting screen automatic control with the PLC; The fifth, this paper is to inquire the possibility of apply the PLC in ship's engine room surveillance system; According to the comprehensive analysis of above actual example of ship's auxiliary machinery automatic system with the technique of PLC; not only can use the technique of PLC to replace traditional relay-contactor control technique in ship's auxiliary machinery automatic system, but also can get the conclusion of the former is more dependable and more clever than the latter, the maintenance is more convenient, thus develop a new path for ship automation.

本文研究的工作主要包括五个部分:第一,根据船舶辅锅炉的工作特点,设计PLC在船舶辅锅炉自动控制系统中的应用方案,主要有PLC选型及输入输出点的设计,画出系统控制梯形图并分析工作原理;第二,根据交流三速变极调速起货机的工作要求,确定采用PLC控制的方法,进行控制系统的硬件设计和软件设计,并分析工作原理:第三,设计了PLC在三相异步电动机控制中的应用,给出了控制梯形图、程序及输入、输出接线图;第四,研究了用PLC实现船舶组合起动屏自动控制的方法,进行了系统的电源设计和软件设计,给出系统工作流程图;第五,探讨了船舶机舱监视系统应用PLC的可行性,将继电器报警线路改为用PLC控制报警,给出系统硬件图和软件梯形图,并分析了PLC机的选型、抗干扰和应用PLC的经济效益和社会效益等问题。综合以上船舶辅助机械自动控制系统采用PLC技术的实例分析,可以得出船舶辅助机械自动控制系统不但可以使用PLC技术取代传统的继电器接触器控制技术,而且前者比后者更可靠、更小巧,维护保养更方便的结论,从而为船舶自动化开辟了一条新的路径。无忧毕业设计网 www.5ubs.cn

Epik High《epilogue》《over》 You got so much to prove hopin' they approve The only thing that's true is all you ever do is do You're movin' shoe to shoe but you're not goin' you stop growin' the moment that you stay at the top The only way is to drop free-falin' down the stairs that you climbed up lined up to freely mount the air But you dare not airdrop tired up like a hairknot hikin' down without a chance of steppin' on a fair rock And so you stand still in a stand still Hands still buildin' castles on a sandhill Man chill is what your friends say but you're not hearin' what little men say Anyway Keep on goin' and taste the stars Keep on growin' and raise the bar You're livin life for the A's down to the Z's After the hill you've got a mountain to seize You are an overacheiver do what it takes till it takes everything you are You are an overacheiver do what it takes till it takes everything you are Who can tell Your living in an organized hell The mansion of your mind just an oversized cell The pressure everything is done to a measure In the sea of competition sunk like a treasure Like a feather fallin' slow spralin' to the floor Strung up like a broken violin to your course Opportunity is knockin' at your door but you never left a welcome mat (It doesn't matter anymore) Or anyhow but you're too late to turn back Fate pushin' you into the wall like a thumbtack Ain't no comebacks in this game of life Roll the dice again Roll it once never twice Keep on goin' and taste the stars keep on growin' and raise the bar You're livin' life for the A's down to the Z's After one drop you've got a fountain to seize You are an overacheiver do what it takes till it takes everything you are You are an overacheiver do what it takes till it takes everything you are You are an overacheiver do what it takes till it takes everything you are Want a break from the world but the world wanna break you The weight makes your backbone curl up and ache you You are an overacheiver do what it takes till it takes everything you are Want a break from the world but the world wanna break you The weight makes your backbone curl up and ache you

登录 |《跋》Epik高《在》你有如此多的证明 hopin '他们批准只有一件事,是真实的你满脑子都是做是什么你的鞋,准予鞋但你不是要你停止growin ''那一刻你呆在上面惟一的办法就是下降 free-falin"下楼梯,你爬了上去自由转会到山的空气但你不敢airdrop累了,就像一个hairknot "没机会 steppin的一个公平的岩石和你伫立于一个静止 buildin手依然在sandhill的城堡你的朋友是什么人寒意但是你不hearin'小男人说无论如何,味道传来的持续的星星继续growin '和酒吧你在生活的一个能Z的你已经得到了山之后,一座山要抓住你是一个overacheiver 做任何事,直到你是什么你是一个overacheiver 做任何事,直到你是什么谁能告诉你的生活在一个有组织的地狱大厦的头脑只是一个超大的细胞所有的压力,对测量在大海中沉没宝藏。像竞争像羽毛的慢spralin因为"到地板上像破碎悬挂的小提琴你的课程机会是knockin '来到你的门前但你从未离开过一个欢迎仪式。无论如何,但你还是太迟了回去"你pushin命运像个图钉墙不是在这场比赛复出的生活滚动骰子滚它又从不两次不断传来的味道和天上的星星继续growin '和酒吧你推荐的生活为一个能Z的你已经得到了一滴后一个喷泉扣押你是一个overacheiver 做任何事,直到你是什么你是一个overacheiver 做任何事,直到你是什么你是一个overacheiver 做任何事,直到你是什么想一下这个世界但是世界想要打破你让你的体重骨干蜷缩和疼痛你是一个overacheiver 做任何事,直到你是什么想一下这个世界但是世界想要打破你让你的体重骨干蜷缩和疼痛

As availing for or equivalent to something, as having the like force and weight 1b to number among, reckon with 1c to reckon or account 2 to reckon inward, count up or weigh the reasons, to deliberate 3 by reckoning up all the reasons, to gather or infer 3a to consider, take into account, weigh, meditate on 3b to suppose, deem, judge 3c to determine, purpose, decide

数算, 计算。。。 1c 考虑,认为 2 猜想考虑,评断各种原因,深思熟虑,好好想想 3 把所有的因素列入考虑,推测或推断 3a 考虑,列入考虑,评估,思考计划 3b 推测,相信,判断 3c 决断,下决心,决定

Eased up globally and prosperously and it influenced that the mainland China investment environment has transferred to difference, the Taiwan trader goes to the mainland China to make the investment and ease up, the wages causes China to work and is generally deficient and organize to promise, but in a situation that ' there are few much monks of gruel ', Chinese workers to begin with the psychology that waits for the right price to sell, force enterprises to improve salary and conditions, The Chinese workers loyalty influence Taiwan treader invest to China seriously.

本研究根据管理制度、工作满足以及组织承诺等方面的关连性,透过问卷方式收集台资企业在大陆地区的员工现况,并以统计分析的变异数分析以及回归分析探讨管理制度、工作满足以及组织承诺等议题中各相关因子与构面间的差异性与相关性,并依据分析结果,分析大陆劳动的个人属性、管理制度与工作满意度、组织承诺间的相关性,作为台资企业在大陆地区人力资源的筛选以及降低如何劳动离职率的参考。

The first helps you pack, you have a lot of advantages, cannot mirror on your photograph come out, you speak what give to the other side directly, feel embarrassed expression comes out, undertake conduct propaganda to you by our soubrette website, achieve your desire, the 2nd problem, have a problem e.g. you bad to ask, e.g. settle on of a girl a boy, this kind of problem, have oneself advocate house, this kind of problem is bad at the beginning to ask, through our website side you ask, after bilateral appointment was over, do not know the other side normally how, main service is a phone the service basically is this respect, our group is the white-collar that comes 45 years old in 25 years old, the time that the mainest is them is exceedingly valuable, our service is aimed at the service that reflected at 2 o'clock was to reflect two problems, the first is the time that saved them, make them not beautiful many time, spend several hours to enter these activities, if if your disposition compares undemonstrative word, only 3 two people undertake inquiring to you, if we serve with soubrette website, have man-to-man service, improve your efficiency that match, we everyday add a member newly to 20 thousand increase a membership newly, the growth rate of our income is very rapid, we are the first soubrette websites, we cannot say specific number now, we everyday income is us the summation that 3 the biggest competitors add up is even tall, we and other websites still do not have gain euqally, the growth rate of our income is very rapid, we can become the website of the first gain of Chinese.

第一个帮你包装,您有很多的优点,你的照片上不能反映出来的,您直接给对方讲出的话,不好意思表达出来,由我们的红娘网站对您进行宣传,达到你的愿望,第二个问题,比如说你有一个问题不好问,比如说一个女孩子看上了一个男孩子,这种问题,有自己的主屋,这种问题一开始不好问,通过我们的网站帮您打听,双方约会完了以后,通常不知道对方怎么,主要的服务是电话服务主要是这方面,我们的群体是在25岁至45岁的白领,最主要的是他们的时间非常的宝贵,我们的服务针对两点体现的服务是体现了两个问题,第一个是节省了他们的时间,使得他们不要花大量的时间,花好几个小时参加这些活动,如果你性格如果比较腼腆的话,只有两三个人对你进行打听,我们用红娘网站服务的话,进行一对一的服务,提高你的匹配的效率,我们每天的新增会员有两万新增会员,我们的收入的增长速度非常快,我们是第一个的红娘网站的,现在我们不能讲具体的数字,我们每天的收入是我们三家最大的竞争对手加起来的总和还要高,我们和其他的互联网站一样还没有盈利,我们的收入的增长速度很快,我们会成为中国第一家盈利的互联网站。

From my brother Severus, to love my kin, and to love truth, and to love justice; and through him I learned to know Thrasea, Helvidius, Cato, Dion, Brutus; and from him I received the idea of a polity in which there is the same law for all, a polity administered with regard to equal rights and equal freedom of speech, and the idea of a kingly government which respects most of all the freedom of the governed; I learned from him also consistency and undeviating steadiness in my regard for philosophy; and a disposition to do good, and to give to others readily, and to cherish good hopes, and to believe that I am loved by my friends; and in him I observed no concealment of his opinions with respect to those whom he condemned, and that his friends had no need to conjecture what he wished or did not wish, but it was quite plain.

从我的兄弟西维勒斯,我懂得了爱我的亲人,爱真理,爱正义;从他,我知道了思雷西亚、黑尔维蒂厄斯、加图、戴昂、布鲁特斯;从他我接受了一种以同样的法对待所有人、实施权利平等和言论自由平等的政体的思想,和一种最大范围地尊重被治者的所有自由的王者之治的观念;我也从他那里获得一种对于哲学的始终一贯和坚定不移的尊重,一种行善的品质,为人随和,抱以善望,相信自己为朋友所爱;我也看到他从不隐瞒他对他所谴责的那些人的意见,他的朋友无需猜测他的意愿;这些意愿是相当透明的。

Brief and powerless is Man's life; on him and all his race the slow, sure doom falls pitiless and dark. Blind to good and evil, reckless of destruction, omnipotent matter rolls on its relentless way; for Man, condemned to-day to lose his dearest, to-morrow himself to pass through the gate of darkness, it remains only to cherish, ere yet the blow falls, the lofty thoughts that ennoble his little day; disdaining the coward terrors of the slave of Fate, to worship at the shrine that his own hands have built; undismayed by the empire of chance, to preserve a mind free from the wanton tyranny that rules his outward life; proudly defiant of the irresistible forces that tolerate, for a moment, his knowledge and his condemnation, to sustain alone, a weary but unyielding Atlas, the world that his own ideals have fashioned despite the trampling march of unconscious power.

人的生命是短暂而脆弱的,不可避免的死亡虽然缓慢,但一定会降临在每一个人身上,阴森而无情,这个全能的物质世界毫不容情地滚滚前行无视善恶,任意摧毁,对与人来说,注定今天要失去最亲爱的亲人朋友,明天自己也要通过黑暗的死亡之门,人的生命存在就在于死亡来袭之前,珍惜那些使他短暂的生命变得高贵的思想,鄙弃对命运的恐惧,对自建的圣坛顶礼膜拜,不受机缘的摆布,摆脱主宰他外部生活的粗暴统治而取得心灵的自由,敢于骄傲地向那些不可抗拒的压力挑战----这些压力对其认识和判断力不过是暂时的----他就像疲惫而不屈的擎天巨神,独自支撑着他自己的理想世界,对这种无意识力量的肆意践踏毫不理会。

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相关中文对照歌词
Learning To Make Love To You
Down To The River To Pray
Who To Listen To
You've Got Me To Hold On To
Good To Me As I Am To You
I Used To Know How To Walk On Water
To Say Goodbye To You
Tryin' To Go To Church
Ain't To Proud To Beg
To Laugh Is To Know How To Live
推荐网络例句

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。