查询词典 titrate

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Titrate and adjust the solution to contain 45.0 mg of FeCl3,6H2O per millilitre by adding the same acidic mixture.

滴定并加此酸性混合物调整，使溶液中含有45.0mg/l的FeCl3，6H2O。

Titrate and adjust the solution to contain 59.5 mg of CoCl2,6H2O per millilitre by adding the same acidic mixture.

滴定并加此酸性混合物调整，使溶液中含有59.5 mg/l的CoCl2，6H2O

Titrate and adjust the solution to contain 62.4 mg of CuSO4, 5H2O per millilitre by adding the same acidic mixture.

滴定并加此酸性混合物调整，使溶液中含有62.4 mg/l的CuSO4， 5H2O

In volumetric analysis, we use many glass vessels such as mortar, test tube, beaker, separate funnel, grade cylinder, pipette, Erlenmeyer, buretteand volumetric flask to ground, dissolve, separate, pipe, titrate samples or dilute samples to volume.

在容量分析中，我们使用很多玻璃容器，如迫击炮，试管，烧杯，单独的漏斗，职缸，移液器， erlenmeyer滴管和容积烧瓶至地面，溶解后，就分开，管，滴定样品或稀释样本量。

In volumetric analysis, we use many glass vessels such as mortar, test tube, beaker, separate funnel, grade cylinder, pipette, Erlenmeyer, buretteand volumetric flask to ground, dissolve, separate, pipe, titrate samples or dilute samples to volume.

在容量分析中，我们使用很多玻璃容器，如迫击炮，试管，烧杯，单独的漏斗，职缸，移液器， erlenmeyer滴管和容积烧瓶至地面，溶解，就分开，管，滴定样品或稀释样本量。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。