查询词典 titer

Objective To explore the clinical value of the titer of serum HP antibody in patients with gastric diseases.Methods Serous HP antibody was detected with ELISA and HP was cultured,tested with urase,and stained with Gram method on smears which was observed microscopically and compared with proteus protein-enveloped test.Results ELISA showed high sensitivity and specificity.Conclusion Serous ELISA detection of HP antibody can be used widely in clinical diagnosis and epidemical screening without endoscopy and biopsy.

目的 探索胃部疾病患者血清中的幽门螺旋菌HP抗体滴度的临床价值方法应用酶联免疫吸附试验ELISA法检测HP抗体与HP培养，尿素酶试验和涂片革兰氏染色作镜下形态检查相比较，并同时与变形杆菌蛋白包被测定法作比较结果显示该法具有敏感特异性强的特点结论该法无需常规内镜检查，活检查HP而单纯用血清学检查，可广泛应用于临床诊断和流行病学人群普查

Although the investigated oral BCG vaccine formulation showed weak immune response to elicit IgG titer, the PPD skin test in guinea pigs by oral dosing of 4 mg BCG vaccine formulation acquired about equal or higher response than that by 0.5 mg dose by subcutaneous vaccination.

所研制之口服BCG疫苗制剂在诱发IgG之免疫反应较弱。但在结核菌素PPD之天竺鼠试验，显示口服BCG疫苗制剂4 mg可达到与皮下注射0.5 mg相同或较佳的反应。

The result showed that the HA titer of virus in allantoic fluid reached its maximum 1:256~1:512 in the condition that the 9-day chick embryo received 50 times dilution of 1:64 GPMV and cultured for 48~72 h in 37℃.

结果表明将血凝价为1：64的鹅副粘病毒稀释50倍后，接种9日龄鸡胚，37℃培养48~72 h获得的尿囊液血凝价可达1：256~1：512，平均尿囊液量可达9.4 mL/胚。

Virus was also cultured in allantoic cavity of chicken embryo with statistically significant HA titer at 1:6.8 as compared with virus control group (P.01) incubated 24 hours after injected with MEK first then injected with IBV.

在鸡胚尿囊腔中，先注入MEK孵育24 h后，再注入B型流感病毒的鸡胚也培养出病毒，HA滴度为1：6.8，与病毒对照组比较P.01，有统计学意义。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Following detection of a high titer of ANAs (eg 1:160), various subtypes are determined.

继发现高滴度的鸭（如1：160 ），各种亚型决心。

There was no cross-reaction of the prepared raw antigen with the positive sera from dogs infected respectively with Ancylostoma caninum,Toxocara canis and Demodex canis and the highest titer of the positive serum from dogs infected with D.immitis was 1∶1024 detected by the dot-ELISA,which had good reproducibi-lity.

为快速诊断犬恶丝虫病，建立了犬恶丝虫抗体dot-ELISA检测方法，并与阻断试验、交叉反应试验、重复性试验、琼脂扩散试验和平行对照试验进行了比较。

The highest titer of anti-CT mAb (CT-3A and CT-9A) determined by indirect ELISA was 1:15,625. Secondly, we made the colloidal gold from the chloroauric acid (HAuCl4) and labeled anti-CT mAb with the 20 nm colloidal gold.

第二部分进行胶体金合成工作，并成将霍乱毒素单株抗体与20 nm胶体金进行抗体染色，进一步应用於霍乱毒素免疫分析试片之开发上。

The titer variation to juvenile hormone of female worms was detected by GC-MS system, from which there was an obvious correlation between variation regularity to juvenile hormone and vitellogensis's regularity in Chrysopa septempunctata.

通过GC-MS系统测定了大草蛉雌虫体内保幼激素Ⅲ滴度的变化，从中可以看出大草蛉体内保幼激素滴度的变化与卵黄蛋白发生存在明显的相关性。

The titer variation to juvenile hormone of female worms was detected by GC-MS system, from which there was an obvious correlation between variation regularity to juvenile hormone and vitellogensiss regularity in Chrysopa septempunctata.

通过GC-MS系统测定了大草蛉雌虫体内保幼激素Ⅲ滴度的变化，从中可以看出大草蛉体内保幼激素滴度的变化与卵黄蛋白发生存在明显的相关性。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。