查询词典 titer

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

The ELISA titer is 4 times higher than indirect hemagglutination test result.

间接凝集试验与ELISA方法的比较试验表明，ELISA法比间接血凝试验敏感性高4倍以上。

Results The inhibition rates of hemagglutination titer were 0.75, 87.5, and 100 at the ACT concentrations of 6.7, 13.4, 26.8 and 53.6, respectively.

结果：ACT浓度为6.7，13.4，26.8和53.6的血凝滴度抑制率分别为0.75，87.5和100。

Results: Mouse body weights were significantly lower in high and low dose groups than that of control group (P.05); Spleen index in middle dose group (P.05) and hemolysin titer in high dose group (P.01) were obviously higher than those in control group.

结果：与对照组比较，高、低剂量组小鼠体重明显下降(P.05)，中剂量组小鼠脾脏指数明显升高(P.05)，高剂量组小鼠的溶血素效价显著升高(P.01)。

This protein reduced antibody titer in mice immunized with BSA. Following the deletion of sCD152, however, there was no obvious inhibitory effect on the primary immune response triggered by the nonrelevant antigen OVA, indicating that sCD152 had an antigen-specific immunosuppressive effect. We designed a new vector, pPIC-HE-sCD152, to get entirely native sCD152 without His-tag.

为了获得既带有纯化配体表位又带有可剪切序列的可溶性sCD152蛋白，我们设计构建了含有His-tag和肠激酶识别切割序列DDDDK的sCD152表达载体pPIC-HE-sCD152，结果这一表达载体在毕赤氏酵母中同样高效分泌表达出目的蛋白，大量纯化后用肠激酶酶切去除His-tag，用以进一步功能实验，初步结果表明具有体外细胞免疫抑制功能。

The number of polyhedral inclusion bodies was increased 32.3% and the titer of extracellular virus was increased 20.9%.

在换用的培养液中添加1％TPB可以提高病毒产量，多角体产量增加32.3％，游离病毒粒子的滴度提高20.9％。

Main clinical symptom of poult gooses were depressed, retardation in reaction, food appetite decreased, alo laxata; and the average death time was 62.6 h Mark processes in dissection were that spleen was intumescenced, gray necrosis appeared; haemorrhage in pancreas, gray necrosis appeared; haemorrhage in intestinal tract, anabrotic; the HA titer of the virus isolated from these samples could reached as high as 2^8, and had the chicken red blood cell agglutination and lys agglutination ability.

雏鹅平均死亡时间为62.6 h。特征性剖检病变为脾脏肿大，有灰白色坏死点；胰脏出血，有灰白色坏死点；肠道出血，溃疡；病料分离培养的病毒液HA价高达28，能凝集鸡红细胞，并出现解凝现象。

As an ideal prime immunization vaccine,the DNA vaccine has the advantages of high purity,continuous expression of antigens with low dose, repeatable immunization and inducement of memory CTL with high titer.Similarly, recombinant virus can not only highly express the target antigen,simulate the inducement process of virus infection on body immunity,and induce various cytokines,but also be beneficial to the interaction of high-affinity antibodies with T cell receptors,and the generation of Th1 reaction and CTL reaction.

DNA疫苗抗原纯度高，持续表达低剂量的抗原，可多次重复免疫以及诱导高效价记忆性细胞毒性T淋巴细胞反应，是理想的用于初次免疫的疫苗；重组病毒能高效表达目的抗原，模拟病毒感染诱导机体免疫的天然过程，能诱发多种细胞因子，而且有利于高亲和性抗体和T细胞受体的选择，可以有效地产生Th1型和CTL反应。

In this experiment, repetitiously intermittent bead-to-bead transfer of cell was chosen to simulate the close and continuous process. During the transfer of 1:4 expansion (the ratio of fresh and confluent microcarriers) for 4 times, it was found that cell density remained stable, while the HA titer showed a slight decrease probably due to the bad culture environments.

由于受实验条件的限制，本实验采用多次间歇加料的方式来模拟连续培养过程，经过4次新老载体1：4比例的放大培养，发现细胞密度可以保持稳定，而病毒产量有轻微下降的趋势，这可能是由于病毒生长环境比较恶劣的缘故。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。