查询词典 titer

It was experimentally found that the average titer of antiserums was more than 12800 by indirect ELISA.

以PA-OVA为包被抗原，采用间接ELISA检测抗血清，其效价达到

Although those DAPs were also found in the hemolymph of non-diapausing larvae, they had higher titer in the hemolymph of diapausing larvae than in the hemolymph of nondiapausing larvae.

虽然它们在非滞育幼虫血淋巴中也存在，但是它们在滞育幼虫血淋巴中的含量明显高于在非滞育幼虫血淋巴中的含量。

Results In general, the anti-HBs titer, ratio of anti-HBs IgG2a to IgGI and number of IFN-γ spot funning cells of mice immunized with YHB-IC were slightly higher than those with CHO-IC, however, the difference was insignificant.

结果 IC诱生的抗－HBs效价、抗－HBs IgG2a/IgGI的比值及IFN-γ形成细胞数，YHB-IC组总体上略高于CHO-IC组，但差异无显著意义。

Methods High titer and specific antibody was obtained by immunizing rabbits with recombinant murine leptin many times.

用人工重组的小鼠leptin多次免疫兔子获取高效价、高特异的小鼠leptin抗体。

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

At this time,priming with 25 IU/ml mouse IFN about 12-24 h and then induced with NDV and superinduced with cycloheximide(20μg/ml) and actinomycin D(2μg/ml),the titer of IFN in the media would be 10~5IU/ml approximately, or 10~5IU...

用25IU/ml鼠IFN起动12—24小时，用NDV作诱生剂，并采用环己亚胺(20μg/ml)和放线菌素D(2μg/ml)进行超诱导，IFN产量可高达1×10~6IU/ml左右，比活接近1.3×10~5IU/ml蛋白。

The specific McAb against dimethoate is obtained in this experiment, but its titer and affinity constant are low so that it is not up to standard enough to prepare kit using the McAb.

通过杂交瘤技术，获得1株特异分泌抗乐果McAb的杂交瘤细胞，但McAb效价、亲和力都较低，与制备试剂盒的要求尚有较大的差距。

Under the optimum concentration of 1.5% glycerol in fermentation medium and feeding of 1% glycerol as precursor after 60 h of incubation, the titer of 2,668 μg/ml was obtained in shake flask culture, an increase of 7.4 times compared with the original strain.

以XC-5-48菌株为试验菌株，当培养基中甘油初始浓度为1.5%，并且在发酵60h时补加1.0%的甘油，摇瓶发酵的效价可达2668μg/ml，较出发菌株(318μg/ml)提高了7.4倍。

High-titer antisera were obtained, which successfully detected the antigen at a dilution of 1:27 000 by Western blot analysis.

所得到的抗血清能够在稀释27000倍后成功用于免疫印迹检测。

Immunization of rabbitsUse suitable immunizing method to obtain antisera with high titer and highly activated spleen cells for fusion.

实验主要内容包括：(1)240E细胞的培养根据生长曲线的绘制，调整240E细胞生长状况，获得适合融合的240E细胞。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。