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This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.As a result,hypha culture time 20 h,penicillinG Na 0.5 μg/mL,lysozyme 1 mg/mL,lytic time 30 min,SMM as osmotic pressure stabilizer,ATB culture medium of regeneration culture mediu...

结果表明，酒酒球菌SD-2a原生质体制备的最佳条件为：菌龄20 h，青霉素G钠质量浓度0.5μg/mL，酶质量浓度1mg/mL，酶解时间30 min，渗透压稳定剂采用SMM缓冲液，再生培养基采用添加0.7 mol/LKCl的ATB培养基，再生培养时采用双层培养基厌氧培养，可以保证较好的形成率和再生率。

In the seedlings time, the content of chlorophyll decreased by the deficiency of B, but the content of chlorophyll in the high efficiency cultivar decreased slowly than that of the low efficiency cultivar. This suggested that the leaves of the high efficiency cultivar can capture more light energy and the photosynthetic rate of the high efficiency cultivar decreased slowly. In the bolting time and blooming time, after two weeks of B deficiency treatment, the content of chlorophyll in the top leaves of the high efficiency cultivar decreased, and in the late time, the content of chlorophyll decreased slowly. But, the content of chlorophyll in the bellow leaves of the two cultivars had little changes. The reason may be that B in the high efficiency cultivar distributed to the propagative organ in the late growth, and the leaves were short of B.

苗期，缺硼导致甘蓝型油菜色素含量降低，且高效品种色素含量下降的幅度小于低效品种，表明营养生长阶段，同等程度的缺硼条件下，高效品种叶片对光能的收集能力以及光合速率下降的程度相对较小；苔期和花期，在两周的缺硼处理后，高效品种上部叶色素含量依然呈下降趋势，但随着生育期的推移，其下降幅度减小；而高效品种下部叶和低效品种叶片含量变化不大，可能是甘蓝型油菜进入生殖生长阶段后，高效品种将硼更多地分配到繁殖器官，而其叶片却承受了相对较大的缺硼胁迫。

Total gastrointestinal transit time, left colonic transit time, right colonic transit time and rectosigmoid colonic transit time were measured by the simplified method of radiopaque makers.Meanwhile, anorectal vector manometry was performed. The Constipation group was subdivided into Slow-transit group and Normal transit group according to the TGITT to explore the value of anorectal manometry in different types of constipation.

对28例便秘儿童和43例正常儿童进行全胃肠传输时间、左半结肠传输时间、右半结肠传输时间和直肠乙状结肠传输时间测定和直肠肛管向量测压(包括肛管压力、向量容积和对称指数)。

The 352 valid samples were analyzed by SPSS. The results showed there was no distinct corresponding relationship between the product attributes and reaction time. The different key attributes from questionnaire importance rating and the shortest reaction time standards were used to regressively analyze the results of customers' overall rating (such as overall satisfaction,objective quality, recommend intention).The results indicated that the coefficiency of regression of the special attributes chosen from reaction time to overall rating was distinct, while the coefficiency of the special attributes chosen from importance rating to overall rating was not. The main conclusions are: 1. Regarded attributes can be obtained by the reaction time of brand performance rating.

本实验的有效样本为352个，通过SPSS软件进行分析统计，数据统计结果显示，在品牌表现评价反应时与其重要性问卷评定结果的相关系数的检验中，并未发现品牌表现评价反应时与品牌属性重要性评定结果之间的显著相关；将由问卷重要性评价最高标准所得到的关键属性与通过品牌表现评价反应时最短的标准所选取的关键属性的品牌表现分别对消费者的总体评价结果（如&总体满意度&、&主观质量&、&推荐意向&）进行回归分析，发现通过品牌表现评价反应时方法选取的特殊关键属性（与问卷重要性评定法选取不一致的关键属性）的品牌表现对总体评价结果的回归系数显著，而重要性评定问卷法选取的特殊关键属性（与品牌表现评价反应时法选取的不一致的关键属性）的品牌表现对总体评价结果回归系数不显著。

Therefore, I restack many time points up on a plane of time axis and view "time" pantoscopicly to pursue the face fading in a time flow.

於是，我将多个时间点，再次重新堆叠於同一个时间轴的平面，将时间用广角的意识去观看，追求那个已模糊在时间流动中面容。

At first, this algorithm obtains rough similar series by clustering the broad moving average; Second, based on this, we can construct the similarity degree of time series trend, by using this similarity degree of time series trend, the users can do riddling secondly. At last, through calculating the distance of the residual time series, accurate similar time series are reached.

该方法首先利用对时间序列广义移动均值的聚类进行相似搜索的粗匹配；然后通过构造时间序列趋势的相似度，利用用户对趋势相似度的要求可以进行第二次筛选；最后对剩余的时间序列进行距离计算，就可以获得最终符合用户需求的相似时间序列。

The coast forest onion onion, in green grass cushion cushion, the lake the blue water is clear, near the lake alley, the pebbles shoal, the stair slope protection, the countless changes, causes the lake shore smoothly to stand erect from time to time, the edges and corners are distinct, from time to time the mountain riddlings fold, completely relaxed.

沿岸林木葱葱、绿草茵茵、湖中碧水粼粼，临湖小路，卵石浅滩，台阶护坡，变化无穷，使湖岸时而平滑直立，棱角分明，时而山石层叠，令人心旷神怡。

Real time recording/reproduction information for ensuring real time recording/reproduction is stored in a file control information area, in each real time file, or in a sperate file, and real time recording/reproduction attributes are assigned to the file.

用于保证实时记录/重放的实时记录/重放信息被存储在每一个实时文件或一个单独文件中的一个文件控制信息区，并且实时记录/重放属性被指定给该文件。

Firstly, I arranged the time properly, adjusting rest time, sutdy time and sportful time.

首先，合理安排时间，调整好作息时间，分配好学习、工作、娱乐的时间。

The wavelet time-frequency properties of the uncracked rotor and the cracked rotor were discussed by the continuous wavelet time-frequency analysis algorithm. A new algorithm using the wavelet time-frequency analysis algorithm to detect the crack was proposed. The influence of the sampling frequency on the wavelet time-frequency analysis algorithm was analyzed through the numerical simulation research; the valid sampling frequency was presented.

利用连续小波时频分析方法，讨论了裂纹转子与无裂纹转子的小波时频特性，提出了利用小波时频分析方法识别裂纹的新方法；数值仿真研究了采样频率对小波时频分析方法的准确性和有效性的影响，并给出了建议性的采样频率。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。