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In mouse peritoneal macrophages. Methods Mouse peritoneal macrophages were cultured in the presence of heat-killed yeast-phase PM for 24 h, and the average fluorescence intensity of TLR-2, TLR-4, and Dectin-1 in the macrophages was detected using flow cytometry. Fluorescent staining of the macrophages was performed to observe the fluorescence of TLR-2, TLR-4, and Dectin-1 with confocal microscopy. TNF-α mRNA in the cell culture supernatant was measured with real-time PCR, and TNF-α protein detected using enzyme-linked immunosorbent assay.

马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h，采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度；共聚焦显微镜观察荧光染色的受体；ELISA法测定培养液上清中TNF-α的浓度；Real time PCR检测不同时间段TNF-α的mRNA表达。

Rowling finished up her Harry Potter saga, Russia's iron-willed leader is TIME's pick as Person of the Year.

Rowling完成她的哈利波特传奇的一年，俄罗斯的强势领导人被TIME选中为年代人物。

In another way, the technology of FQ-PCR (real-time fluorescent quantitative PCR) was used to measure the ratio of white nucleus occupying the whole nucleus in 34 light-yellow strains. Among them, the ratio of yellow nucleus and white nucleus in 19 strains was access to 1:1, while the other strains showed that the copies of yellow nucleus exceeded white ones.

另一方面，本实验利用FQ-PCR(teal-time fluorescent quantitative PCR)技术测定了34个浅黄色菌株中白色核数目占总的核数目的比值，其中有19个菌株白色核的拷贝数与黄色核的拷贝数接近于1：1的关系，15个菌株中黄色核的拷贝数要大于白色核的拷贝数。

The established event-specific real-time PCR detection systems in this study are suitable for the identification and quantification of these GM maizes.

再辅以即时聚合酶链锁反应( real-time PCR )检测基因改造玉米含量，将有助於建立基因改造玉米产品标示管理。

These are the accumulated operating times within the cycle, for example, the odometer mileages on a truck when it failed or the time elapse meter on a device reset to zero at the beginning of a cycle.

这些数据是在该周期内的累积操作时间，如卡车在失效时的行驶里程，或者 the time elapse meter on a device reset to zero 在周期开始时复位置0。

Convert Seconds to Other Time Metrics - This function offers seconds conversion up to five levels: munites, hours, days, weeks, years.

Convert Seconds to Other Time Metrics这个程序可以将以秒计算的时间转换为5个等级：分钟、小时、天、周、年。

I have to say that this is the sweetest time ...

我不得不说这是 sweetest time。。。

Seeks supplier Negotiation Purchasing Channel Order Time Payment Small the Yards shipyard to the main big product, like the main engine, the auxiliary engine, the pot wheel, the servo, its purchase List already relatively was fixed, but regarding AL this kind of two kind of products, its purchase behavior was decided the experience which used in the sales personnel's introduction as well as the same profession.

请帮忙翻译中翻英的关于造船厂的非常感谢！寻找供应商 Negotiation Purchasing Channel Order Time Payment Small Yards 船厂对主要大的产品，如主机、付机、锅轮、舵机，其采购的List已经相对固定，而对于AL这一类的二类产品，其采购行为取决于销售人员的介绍以及同行业中使用的经验。

However, smart compilers, well-tuned interpreters, and just-in-time bytecode compilers can bring performance close to that of native code without threatening portability.

但是，智能的编译器，优秀调整的解释器，和 just-in-time 字节码编译可以使得其性能接近于本地代码而不会笨重。

Time-bound: Give yourself a clearly defined end date to achieve your goals by.

u Time-bound―有期限的：给自己定下一个达成目标的截止日期。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。