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Immunotherapy is a kind of tumour treatment with bright future on improving power for killing tumour cell by starting active immunity energy of self in order to monitor cell variety, eliminate remaining carcinoma focus, to avoid transition and relapse,to increase cure rate of tumour, to prolong living time and to improve living quality etc'4'.

肿瘤特异性免疫治疗是通过启动自身主动免疫能力和提高杀伤肿瘤细胞能力,以达到监视细胞异变、消除手术残留癌灶、防止转移复发、提高肿瘤治愈率、延长存活期和提高生活质量的一种很有前途的肿瘤治疗方法。

Comparisons between the cell lines were made on the basis of time to observed cytopathic effects, ultra structure changes, titer in 50% tissue culture infectious doses, and the alterant distribution of DNA content in cell cycle. The results showed that, under identical conditions, various degrees of CPE could be found in the Hep-3B, A549 and NIH 3T3 36h post infection of BTV-10, while there was no CPE could be observed in HEL.

随后在对BTV-10体外感染特性的研究中,我们在相同条件下,将该病毒接种于1株原代人胚肺成纤维细胞(Human embryonic lung fibroblast cells,HEL)和3株传代细胞株:人肝癌细胞(Human hepatic carcinoma 3B cells,Hep-3B);人肺腺癌细胞(Human lung carcinoma A549 cells,A549);鼠正常成纤维细胞(Normal mouse fibroblastscells,NIH 3T3)。

Cell cycle uncoupling indued by DNA damage;2. Effect of 5-flurouracil on cell cycle uncoupling of Jurkat and EL-4 cell lines;3. This paper studies the sludge reduction effect caused by sludge concentration,sludge retention time and temperature in the uncoupling reactors in OSA process by both single-factor and orthogonal method.

采用单因素试验和正交试验的方法分别研究了城市污水污泥减量OSA工艺中解偶联池中污泥浓度、污泥在解偶联池反应时间、解偶联池温度对污泥减量效果的影响,发现增加解偶联池污泥浓度或延长污泥在解偶联池的反应时间或增加解偶联池温度都将导致解偶联池ORP线性下降,且污泥表观产率系数Yobs与ΔORP或各因素之间分别遵循Yobs=-0。

The results of cell growth curves showed that allvoltages inhibited cell proliferation and prolonged cell multiplication time.

其中,生长曲线结果表明,所有的电压都抑制了 HeLa 细胞的生长增殖,延长了细胞的倍增时间。

Methodshexamethyldisiloxane polymer film was obtained by plasma polymerization method on coverslips. oxygen (o2) plasma treatment was employed to modify the surfaces with different time so that a series of surface with different wettability were produced. l929 cell were cultured on these surfaces to observe the cell attachment rate and cell spreading in 6 and 12 hours.

使用薄膜等离子体聚合法在圆形盖玻片表面生成六甲基二硅氧烷(hexamethyldisiloxane, hmdso)薄膜聚合体,然后用低温氧等离子体轰击聚合体表面,根据轰击时间的不同形成一系列的不同润湿性表面,将成纤维细胞系l929接种于不同润湿性表面,观察6h、12h时细胞的贴附率,并观察细胞的形态。

Methods Hexamethyldisiloxane polymer film was obtained by plasma polymerization method on coverslips. Oxygen (O2) plasma treatment was employed to modify the surfaces with different time so that a series of surface with different wettability were produced. L929 cell were cultured on these surfaces to observe the cell attachment rate and cell spreading in 6 and 12 hours.

使用薄膜等离子体聚合法在圆形盖玻片表面生成六甲基二硅氧烷(Hexamethyldisiloxane, HMDSO)薄膜聚合体,然后用低温氧等离子体轰击聚合体表面,根据轰击时间的不同形成一系列的不同润湿性表面,将成纤维细胞系L929接种于不同润湿性表面,观察6h、12h时细胞的贴附率,并观察细胞的形态。

ResultsMTT assay showed that various concentration of VM26 could effetely inhibit the growth cell; according to the results of flow cytometry, some typical sub diploid peaks were observed,the distribution of cell cycle phase changed at various time, the cell cycle of G2/M phase was arrested within 48 hours.

结果在一定浓度范围内,细胞的生长抑制率随着浓度的增加而增加,细胞的凋亡率随着时间的增加而增加,细胞周期被阻滞在G2/M期,在48小时达到高峰。

Ocean Rhodotorula is a single cell eukaryon biology, and cannot the movement, commonly the cell assume the cylinder, the ellipse or the circular generally, the cell long approximately 3-10um, wide approximately 15-5.5um, its shape and the size are raised the cultivate condition and the raise time will influence occur frequently the change, its colony is the pink.

海洋红酵母为单细胞真核生物,不能运动,细胞一般呈圆柱形、椭圆形或圆形,细胞长约3~10um,宽约15~5.5um ,其形态和大小受培养条件和培养时间的影响常常会发生改变,其菌落为粉红色。

Based on setting up mathematical model of wood fiber cell structure, this paper studied on the variation description method of wood fiber cell structure, at the same time, this item set up the judging rule of the smallest cutting thickness of micrometer wood fiber cell splitting, gaining the high strength MFB of poplar whose modulus reach above 5171MPa.

本文在建立木纤维细胞结构数学模型的基础上,进行了木材纤维细胞结构劈裂描述方法的研究,建立了微米木纤维细胞劈裂的最小切削厚度判定原则,MFB的实验研究获得了弹性模量达到5171MPa以上的高强度试件。

He terminal cell apoptosis depended on the time of complete degradation of protoplast in cell.During PCD process,all kinds of cell organelles in protoplast promoted secondary wall deposition at different degree.

EM观察结果表明纤维、基本薄壁组织细胞、导管分子的分化过程均是典型的衰退过程,它是由次生壁的沉积和原生质体的衰退这两个密切相关的过程组成。

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