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The final acidity of yogurt ferment by the two bacteria was 57.65 T and 54.43 T respectively.Because the -galactosidase produce by starter is a endoenzyme it must abstract after the cell wall broken. This experiment compare the different method of cell wall broken and research on the influence of temperature and time on condition of cell wall broken.

由于发酵剂菌种产生的糖代谢酶,β-半乳糖苷酶是胞内酶,通过对渗透压破壁和不同浓度的氯仿和甲苯两种有机溶剂破壁方法的比较,以及破壁所需温度和时间的研究,确定采用5%氯仿,45℃保温8~12h对菌体进行破壁,β-半乳糖苷酶的活力采用AOAC提出的标准进行测定。

Results Stable drug-resistant SW1990/GZ cell strain was established by culturing with gemcitabine for 9 months. The morphology and growth characteristics of the cell strain changed remarkably. The cells shrinked and became rounder; its endoplasm expanded; granular substances increased; and the doubling-time was prolonged. Resistance of the cell line to gemcitabine, fluorouracil, adriamycin, and mitomycin significantly increased. The TrxR activity of the drug-resistant cells was increased markedly.

结果 药物培养9个月后,得到稳定传代的SW1990/GZ耐药细胞株;其形态学、生长特征等均发生了明显变化,细胞变圆、体积缩小、内质网扩张、颗粒样物质增多及细胞倍增时间延长;对吉西他滨、5-氟尿嘧啶、阿霉素和丝裂霉素的耐药性均显著增加;耐药细胞的TrxR活性也明显增高。

Domestic entrance, adult college entrance examination, self-examination and the examination of various institutions, some criminal elements to use cell phones to cheat; in the test set MDPB-04-based mobile phone signal isolator, can be effective in blocking the process of examination of the types of mobile phone signal transmission, because cell phone signals within the scope of the test was cut off, any test can not answer the phone, view from outside the examination room phones, text messages; can not make calls, send text messages; guarantee test the order of category 2 intermediate and high school students to use cell phone text messages during school hours in the classroom hair set edsun-2008-type devices to block mobile phone signals, through the mobile phone signal blocking devices to block mobile phone signals, the mobile phone so that students can not receive text messages, send text messages to ensure that students can concentrate on in the classroom full time in a limited guarantee that the quality of teaching.

国内高考、成人高考、自学考试及各类大专院校在考试过程中,一些不法分子利用手机作弊;在考场中设置MDPB-04型手机信号隔离器,可在考试过程中有效阻断各种类型的手机信号的传输,由于手机信号在考场范围之内被隔断,考场内的任何手机都无法接听、查看来自考场以外的电话、短信息;也无法拨打电话、发送短信息;保证考场秩序2类中级、高级中学学生利用手机在上课时间乱发短信在教室中设置edsun-2008型手机信号阻断器,通过手机信号阻断器的阻断手机信号的功能,使学生的手机无法接收短信、发送短信,保证学生能够在课堂上集中精力,充分在有限的时间内保证教学质量。

Domestic entrance, adult college entrance examination, self-examination and the examination of various institutions, some criminal elements to use cell phones to cheat; in the test set MDPB-04-based mobile phone signal isolator, can be effective in blocking the process of examination of the types of mobile phone signal transmission, because cell phone signals wit你好n the scope of the test was cut off, any test can not answer the phone, view from outside the examination room phones, text messages; can not make calls, send text messages; guarantee test the order of category 2 intermediate and 你好gh school students to use cell phone text messages during school hours in the classroom hair set edsun-2010-type devices to block mobile phone signals, through the mobile phone signal blocking devices to block mobile phone signals, the mobile phone so that students can not receive text messages, send text messages to ensure that students can concentrate on in the classroom full time in a limited guarantee that the quality of teac你好ng.

国内高考、成人高考、自学考试及各类大专院校在考试过程当中,一些非法分子利用手机做弊;在科场中设置MDPB-04型手机信号断绝器,可在考试过程当中有用阻断各类类型的手机信号的传道输送,因为手机信号在科场规模以内被隔绝,科场内的不论什么手机都无法接听、查看来自科场以外的电话、短信息;也无法拨打电话、发送短信息;包管科场秩序2类中级、高级中学学生利用手机在上学时间乱发短信在教室中设置edsun-2010型手机信号阻断器,路程经过过程手机信号阻断器的阻断手机信号的功效,使学生的手机无法吸收短信、发送短信,包管学生能够在课堂上集中精力,充分在有限的时间内包管讲授质量。

Methods The model of intravasation migration of cancer cell in vitro was used. The fibrosarcoma cell HT1080 was labelled with fluorescence, and the fluorescence guantities in 24-pore plate through collagen membrane and HUVEC single cells layer were detected in different time to eveluate the intravasation migration of cancer cell.

利用体外癌细胞向血管内游走模型,将纤维肉瘤细胞HT1080经过荧光标记,通过测定不同时间穿过胶原层及HUVEC单细胞层落到24孔板内的荧光量来定量评价瘤细胞血管内的游走情况。

The models of homogeneity and function morphism, is emerging constantly, it is the cell-phone rejected only that floods the market first, while the trade company is immersed in the profit is puzzled and consumers are enjoyable, some producers introduce colors and reject the cell-phone one after another , the cell-phone market is crowded with people at one time, the producer reaps fabulous profits by this too.

同质同功能同型的机型,不断涌现,先是单屏的手机充斥市场,在商家沉浸于"利惑"和消费者还来不及过瘾的同时,一些厂家纷纷推出彩屏手机,一时间手机市场上人头攒动,厂家也借此大捞一把。

breast cancer cell line MCF-7 were marked killed by trametes robiniphila murr in certain concentration. The IC50 of trametes robiniphila murr in three days to MCF-7 was about 0.05mg/ml. Its cell toxin were increased while the touched time was delayed or the concentration was advanced. Trametes robiniphila murr also can advance cell toxin of adriamycin and reverse resistance of MCF-7/Adr.

用MTT 法测定不同浓度的槐耳浸膏作用不同时间下对乳腺癌细胞系MCF-7 的杀伤作用,同时测定应用槐耳浸膏时对阿霉素的增敏作用和耐药逆转作用;结论:金克在一定范围内对乳腺癌细胞系具有明显的杀伤作用,其作用3 天时,IC50 在0.05mg/ml 左右,随着药物浓度升高和作用时间的延长其杀伤作用相应的增强,对阿霉素化疗具有增敏作用和逆转耐药作用。

Lignin deposited were occurred in cell walls of fiber,parenchyma cell,vessel and stone cell,However,the deposition time and period were different.

在基本薄壁组织细胞中还观察到CMTs的微管组织中心的存在,它是由微管、不规则的小泡和浓密基质构成的复合物。

Objective:in order to compare the curative effects of using lamirudine and thymosin together with using thymosin only to the paients of chronic hepatitis b.methods:we had chosen 27 medical cases of chronic hepatitis b,which were divided into two groups:15 cases asone group,and 12 cases as the other group.let group a take 100 mglamirudine orally,qd,with half a year as a period of treatment.at the same time givethe patients injections of 100 mg thymosin,qd,for a month.to group b was only takenlamirudine.alt,tbil,the indicators of the virosis of hepatitis b (hbsag,hbeag,hbv-dna)were observed as quatos.results: at the end of the treatment, the rates that groupa and group b made hbsag transform into negative were respectively 26.67% and 16.67%(p>0.05);hbeag:33.33%,8.33%;hbv-dna:53.33%,33.33%(p>0.05).conclusion:combining thymic peptide with 3tc to treat chronic type b hepatitis, thymic peptide will not only promote b-cell to mature and transformation, but also to keep the subpopulation of t-cell in immune system in balance, which can enhance the reaction of matured t-cell to the antigen or other stimulus.

目的:比较拉米夫定(3tc)与胸腺肽联用和单用拉米夫定治疗慢性乙型肝炎的疗效。方法:选择慢性乙型肝炎患者27例,分为联合组15例和拉米夫定组12例;联合组用拉米夫定100 mg,口服,qd,疗程半年,合并用胸腺肽100 mg,静滴,qd,疗程1个月。拉米夫定组单用拉米夫定。以谷丙转氨酶、血清胆红素、乙肝病毒标志物(hbsag,hbeag,hbv-dna)为观察指标,比较不同治疗方案对指标的影响。结果:治疗结束时联合组与拉米夫定组对慢性乙肝患者血清hbsag阴转率分别是26.67%和16.67%(p>0.05);hbeag阴转率分别是33.33%和8.33%(p>0.05);hbv-dna阴转率分别是53.33%和33.33%(p>0.05)。结论:胸腺肽联合3tc治疗慢乙肝,胸腺肽具有促进t淋巴细胞的成熟和转化,并维持免疫系统各t淋巴细胞亚群间的平衡作用,能增强成熟t淋巴细胞对抗原或其他刺激的反应。

Grew on the normal temperature, it was shown that the deposits of calcium antimonate being the indicator for Ca(superscript 2+) localization mainly concentrated within the vacuoles and intercellular spaces and there was also some Ca(superscript 2+) deposits in cell walls. But when Garyota urens L. was treated by the temperature of 2℃ for 48 h, the level of Ca(superscript 2+) increased in cytoplasm and plasma membrane, but decreased in vacuoles and intercellular spaces considerably. At the same time, the ultrastructure of chloroplasts suffered from chilling: the membrane of chloroplasts had been damaged, the layer of thylakoids was exiguous and unclear, the photosynthetic rate decreased evidently. And when Garyota urens L. was treated by the temperature of 2℃ for 120 h, the deposits of Ca(superscript 2+) mainly concentrated within the cytoplasm, nucleus and plasma membrane and there was also some Ca(superscript 2+) deposits in vacuoles, and the ultrastructure of some cells was simultaneously damaged severely: Chloroplasts structure, vacuole membrane and nuclear membrane had been damaged fully, the structure within the cell had become unclear, and the cell only have respiration.

研究结果表明,未经低温处理的董棕幼苗叶肉细胞,焦锑酸钙沉淀颗粒大量出现在液泡和细胞间隙中,细胞壁中也可见少量沉淀,而细胞基质中则看不到焦锑酸钙沉淀;经2℃ 48 h低温处理后,细胞基质和细胞膜上焦锑酸钙沉淀增加,而液泡和细胞间隙中的焦锑酸钙沉淀则显著减少,并且超微结构已初步显示出寒害的特徵,叶绿体外膜部分破损,类囊体片层稀疏且排列不规则,光合速率明显下降等;经2℃ 120 h低温处理后,细胞间隙内的焦锑酸钙沉淀极少,有的也紧贴在细胞外壁上,而细胞基质和细胞膜上则分布有非常多的焦锑酸钙沉淀,在核基质和液泡中也可见到少量的焦锑酸钙沉淀,并且超微结构遭到了显著破坏,叶绿体结构完全被破坏,核膜与液泡膜严重破损,内部结构模糊,细胞只表现为呼吸作用,不进行光合作用。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。