查询词典 time cell

The ultrastructural changes during the development of sieve element companion cell complex (SE/CC complex) in Populus deltoides were investigated using electronic microscopy. The result showed that SE and CC tome from the same secondary phloem mother cell. The development of sieve element went through three stages, i. e. immature, mature, and degenerate stage. In immature stages the sieve element presented radial expansion, cell wall thickening, and production of sieve plastid and P-protein. The cytoplasma selectivly autolysed in mature stage. At the same time, karyoplasm was dispersed, and nuclear envelope was not clear, or karyoplasm was disorgnized, and nuclear envelope was clear. The karyopalsm finally turned into P-protein. In degenerate stage, the cytoplasm completely disappeared, and the function was lost.

利用电镜技术研究美洲黑杨次生韧皮部筛管和伴胞发育过程中细胞超微结构的动态变化，观察到筛管和伴胞由同一个次生韧皮部衍生细胞分裂分化形成筛管约发育历经未成熟期、成熟期和衰退期的变化未成熟期筛管分子表现为细胞的径向扩展、壁的增厚、筛管质体和P-蛋白质的产生；成熟期筛管分子细胞组分发生选择性自溶，细胞核核质弥散状，核膜不清晰，或核膜清晰而核物质降解，最后细胞核完全解体转化为P-蛋白质类的物质；衰退期筛管分子细胞质完全解体消失，失去功能。

They existed as dosage-effect and time-effect. All of these results proved that Sertoli cell was the first target cell, spermatogenic cell was the second one and mitochondrion was the target. Otherwise, we proved this compound can disturb the development and growth of sperms, influence the activity and amount of sperms and increase the deformation ratio.

结合组织病理和性激素及酶活性的改变，揭示该化合物睾丸毒性的靶细胞是支持细胞，其次是生精细胞，作用的靶点是细胞内的线粒体，此外还证明该化合物干扰精子的生长和发育，影响精子的活力和数目，增加畸形率，是雄性精子畸变的诱导剂。

Virus removal efficiency is determined by the real time PCR and inactivation efficiency is determined by cell-based infectivity assay.Enveloped DNA virus removal and inactivation: Real time PCR indicated that method based on gold magnetic particles coupled with antiserum has shown great superiority on virus removal. Cell-based infectivity assay indicate pasteurism has shown superiority on virus inactivation.

对两种指示病毒分别进行巴氏灭毒和偶联抗血清金磁颗粒处理，实时荧光定量PCR检测核酸变化确定病毒的去除效率，同时进行病毒的细胞感染力实验确定病毒的灭活效率，结果显示：在对DNA有包膜病毒的去除能力上，金磁颗粒法明显优于普通的巴氏灭活法；在对DNA有包膜病毒灭活能力上，巴氏法明显优于金磁颗粒法。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

There are some midst matters in wastewater, for removal of these matters the electrolyze time should exceed 30 min. The experimental results show that the bipolar packed bed cell has best treatment efficiency of phenol wastewater under condition that voltage is 10V, electrolyses time is 30 min, and velocity of flow is 1.03 cm/min.Basic on the study on treating of phenol wastewater, this paper carry on a research on treating of circuitry board film wastewater by bipolar packed bed cell.

本文在以上处理苯酚废水研究的基础上，论文研究了BPBC处理两种不同浓度得脱膜废水，试验结果表明，对于浓度较高的酸化气浮-光酚顿的出水，在外加氧化剂双氧水后，最佳处理工艺：槽电压8V，实际电解时间60min，双氧水的投加量为1倍理论药量，pH值为3，COD的去除率达到60%；在酸化气浮过程采用酸铜废液调节pH值将会降低COD的去除率。

Methods: The model of evaluating T cell proliferation stimulated with polyclonal activators, ConA was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. The effect of a specific inhibitor for p38 MAPK, SB253580, on T cell proliferation on different doses and in different time was estimated by flow cytometry. At the same time, the percentage of proliferating T cells and proliferation index were measured by CellQuest and SPSS10.0 for Windows softwares.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立了在多克隆刺激剂刀豆蛋白A刺激下评价小鼠T细胞增殖的模型，通过流式细胞术分析p38丝裂原活化蛋白激酶的特异性抑制剂SB203580在不同剂量、不同时间对T细胞增殖的作用，并应用CellQuest和SPSS10.0 for Windows软件分析增殖细胞各代所占比例和增殖指数。

The temperature affects the sustained time of secondary sporogenous cell split, the sustained time of microspore mother cell, formation speed and regular of male gametophyte.

温度影响次生造孢细胞持续分裂的时间、小孢子母细胞的持续时间及雄配子体的形成速度和整齐度。

Hallowed Be Thy Name Lyric I'm waiting in my cold cell when the bell begins to chime Reflecting on my past life and it doesn't have much time Cos at 5 o'clock they take me to the Gallows Pole The sands of time for me are running low When the priest comes to read me the last rites I take a look through the bars at the last sights Of a world that has gone very wrong for me Can it be there's some sort of error Hard to stop the surmounting terror Is it really the end not some crazy dream Somebody please tell me that I'm dreaming It's not so easy to stop from screaming But words escape me when I try to speak Tears they flow but why am I crying After all I am not afraid of dying Don't believe that there is never an end As the guards march me out to the courtyard Someone calls from a cell "God be with you" If there's a God then why has he let me die?

我等待在我冰冷的牢房，钟就开始附和我过去的生活反映，并没有太多的时间在5点钟产地来源证，他们把我送到绞刑架的时候我正在运行的砂时低神父来到我的阅读我通过在一个已经非常我错了世界上最后景点的酒吧看看最后的仪式是否可以有一些类型的误差很难阻止恐怖的超越，难道不是结束有些疯狂的梦想，有人请告诉我，我做梦这不是那么容易阻止尖叫逃离，但我的话当我尝试发言，他们流眼泪，但为什么我哭了，毕竟我并不怕死，不要相信但绝不是由于警卫3月我到院子里有人为此呼吁从一个单元格&上帝与你同在&如果有上帝，为何在他让我死吗？

The end of time bell of vibration tells every cell within the body and every cell upon earth that it is time to ascend and return "home" to the Tao.

时间结束』钟声的振动，告诉身体内及地球上的每个细胞，现在是时候提升并回家到道之中了。

Travel time in WC anvils was measured using pulse reflect method at room temperature and under cell pressure up to 3.0GPa.Experimental results indicate that the travel time in the WC anvils decreased linearly with cell pressure.

在常温、实验压力为0～3GPa条件下，利用脉冲反射法测量了纵波在碳化钨压钻中的走时，结果发现，随实验压力升高，碳化钨压砧的走时线性减少。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。