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Methods: Experimental mice were perfused with different dosages of tetracycline. Eighteen hours later, the serum levels of ALT, AST were measured and the liver tissues were observed by photics microscope to find the relative suitable dosage that can obviously induce acute liver injury. Then the suitable dosage was given in the same way, and the levels of ALT, SOD, MDA, GSH-PS, IL-18 in serum were measured 18, 36, 54, 72 h later respectively. The condition of cell apoptosis was analyzed, and the tissues were observed by photics microscope and electron microscope to find the relative suitable time.

先以不同剂量四环素灌胃，18h后检测小鼠血清ALT、AST水平并进行光镜观察，探索导致明显急性肝损伤的相对合适剂量；再以相对合适剂量四环素灌胃，18、36、54、72h后分别检测小鼠血清ALT、SOD、MDA、GSH-PX、IL-18水平及肝细胞凋亡情况，并进行光镜、电镜观察，探索导致明显急性肝损伤的相对合适时间及可能机制。

This research determines the change that analysed patient of mobile sex tuberculosis to treat around CRP, medium clinical import is evaluated in order to discuss CRP in phthisical curative effect. 1.1 average data choose 1 data and method to come from January 2005 August 2007 the patient of mobile sex tuberculosis of my courtyard be in hospital 120, all case of illness all combine the phlegmy smear, diagnose such as germiculture according to sternum of line of clinical expression, X or bosom CT, the tuberculosis that chooses case of illness to all accord with China cure to learn tuberculosis to learn branch to make is diagnosed and treat a guideline [2] , 120 all are patient of mobile sex tuberculosis is treated first, male 78, female 18 ～ is 73 42; age years old, average age (40 ± 6) year old. 1.2 methods are all patient early morning is hollow vein collects blood, the United States is used after detached serum elegant earth up Ci8200 full automatic biochemical analyzer determines, use scattering immunity to determine than chaotic law serum CRP level, and determine at the same time red blood cell sedimentations rate , bacterium of phlegmy n/med tuberculosis is checked of education of n/med tuberculosis bacterium...

本探究测定分析了活动性肺结核患者治疗前后CRP的变化，以探索CRP在肺结核疗效评估中的临床意义。1资料和方法1.1一般资料选自2005年1月至2007年8月我院住院的活动性肺结核患者120例，所有病例均根据临床表现、X线胸片或胸部CT结合痰涂片、细菌培养等确诊，所选病例均符合中华医学会结核病学会分会制定的肺结核诊断和治疗指南[2]，120例均为初治活动性肺结核患者，男78例，女42例；年龄18～73岁，平均年龄(40±6)岁。1.2方法所有患者清晨空腹静脉采血，分离血清后采用美国雅培Ci8200全自动生化分析仪测定，采用散射免疫比浊法测定血清CRP水平，且同时测定红细胞沉降率，痰结核菌检查。。。

Using of agarose-embedment lysis cell wallmethod for preparing intact chromosomal DNA from phytopathogenic fungi is thefirst time.

首次采用凝胶包理破壁法由植物病原真菌分生孢子获得完整染色体DNA，克服了先用裂解酶破壁获得大量原生质体再用琼脂糖包理原生质体的传统制备方法的一些不足。

The optimized QUICK scheme (Hayaes, 1992) is used to calculate the variables on the cell faces. The multi-step full-implicit time marching PISO algorithm is adopted to deal with the coupling of velocity and pressure.

空间界面插值采用Hayasa的优化QUICK格式，每一时层的速度场与压力场采用多步PISO算法耦合，从而保证了较高的时间、空间精度。

This observation provided a strong support to the point that most leaves export the most of assimilates in the light time. Plasmodesmal densities between SE/CC, CC/PP, PP/PP and PP/BSC (bundle-sheath cell) decreased in weak light. Plasmodesmata were observed between CC/SE (nacreous-walled sieve element), PP/BSC in branch veins in normal light intensity, but not in weak light. Thus apoplasmic pathway may be the main mode of transport of assimilates in weak light, however symplasmic pathway may be the main mode of transport of assimilates in normal light intensity.

在筛管/伴胞、伴胞/韧皮薄壁细胞、韧皮薄壁细胞/韧皮薄壁细胞和韧皮薄壁细胞/维管束鞘细胞之间的胞间连丝密度都在弱光条件下下降，在正常光照强度下支脉筛管/伴胞和韧皮薄壁细胞/维管束鞘细胞之间可以观察到胞间连丝，而在弱光下几乎观察不到胞间连丝的存在，所以同化物的运输在弱光条件下可能以质外体运输为主，而在正常光照强度下，共质体运输可能是主要的运输方式。

The goat fetal fibroblast cells were transfected by linear GBCt-PAm with electroporation or lipidosome, and after the identification of PCR, we got 7 lines of progenitive cells that examined positive.The two cell line (M9, M10) had been used for the somatic nuclear transfer for the first time.

将pGBCt-PAm去除原核序列后回收线性片段，采用电击和脂质体转染两种方式转染山羊胎儿成纤维细胞，经G418筛选培养基筛选及PCR检测，最终得到了7株能有效扩增的PCR检测阳性的细胞克隆。

20 Centuries 60 time, american Gristina put forward, all biology material are embedded inside body hind, its surface all can form a kind of biomembrane, bacterial criterion is many in this kind of biomembrane progenitive, the many mucilage outside producing a cell is qualitative (Extracellular Slime Substance, ESS), it is the easy hair sex that causes false body infection and the main factor that study a gender hard.

概要： 20世纪60年代，美国Gristina等提出，所有的生物材料植入体内后，其表面均能形成一种生物膜，细菌则在这种生物膜中大量繁殖，并产生细胞外多粘质物质(Extracellular Slime Substance，ESS)，是导致假体感染的易发性和难治性的重要因素。

Law of MTT of 1MTT law principle is 20 centuries a kind when 80 time development rises fast, handy in order to live thiazole of MTT of reducer of cellular metabolization content detects one kind of Lawei foundation the method of cellular amount, was in above all 1983 by Mosmann[4] rat applies at detecting to the cellular factor such as IL-2 survives to the cell and be affected proliferously in the research of lymphocyte department.

本文就MTT体外应用探究目前状况及其进展作一综述。1MTT法原理MTT法是二十世纪八十年代发展起来的一种快速、简便的以活细胞代谢物还原剂MTT噻唑蓝为基础的一种检测细胞数量的方法，由Mosmann[4]在1983年首先在鼠淋巴细胞系的探究中应用于检测IL-2等细胞因子对细胞存活和增殖的影响。

Results M. penetrans LAMPs stimulated mouse macrophages to produce nitric oxide in dose-and time-dependent manner. And M. penetrans LAMPs inhibited cell prolification in mouse macrophages.

结果 穿透支原体脂质相关膜蛋白能以时间和剂量依赖方式刺激小鼠巨噬细胞产生NO，并且对小鼠巨噬细胞的增殖有一定的抑制作用。

In this study, a new horizontal microinjection system has been established to solve these problems. Because the average time per pronuclear embryo by microinjection has been significantly shortened with the horizontal system, the cleavage rate and 16-cell rate of those microinjected embryos increased markedly.

为了解决这些问题，本研究有针对性地设计了新型的水平微注射系统，利用此系统，可明显缩短常规注射原核胚的平均时间，注射后原核胚的分裂率、8-16细胞胚胎发育率也明显提高。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。