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Methods:The Caco-2 cell monolayer model was used to study the bi-directional transport of daphnetin and the effects of time,temperature,pH,drug concentration and P-gp inhibitor on the daphnetin absorption.Drug concentration was measured by HPLC and the apparent permeability coefficients were calculated.

采用Caco-2细胞单层模型研究祖师麻甲素的摄取和跨膜转运,采用HPLC法检测药物浓度,计算其表观渗透系数,考察时间、温度、pH值、药物浓度以及P-gp抑制剂对祖师麻甲素吸收的影响。

And 1993 . In this thesis, we, for the first time, successfully isolated the full-length cDNA for the ECP 63, an embryogenic cell protein, from carrot (Daucus carota L.).

为实验材料,首次成功地分离了ECP63 cDNA全长,并以此cDNA为探针对其在体细胞胚胎发生和合子胚胎发生过程中的基因表达进行了深入的研究。

Results: NaB inhibited cell proliferation time and Concentration dependently.

结果:丁酸钠对SKM-1细胞生长的抑制呈浓度和时间依赖性。

The effect of N-phosphoamino acids on plant cells'membrane permeability was investigated by osmotic method. It was found that in all N-phosphoamino acids, only some phosphoryl polar amino acids such as DIPPSer, DIPPThr, DIPPHis, DIPPAsp, DIPPGlu, DIPPAsn and free serine, threonine could increase the water permeability in plant cell membrane. For example, after 20min. treatment by 1. 5mM DIPPSer, the deplasmolysis time of onion cells was shortened 50%.

三、通过透性实验研究了N-磷酰化氨基酸对洋葱、豌豆等植物细胞膜水透性及溶质透性的影响,发现在所有的N-磷酰化氨基酸及自由氨基酸中,仅有部分N-磷酰极性氨基酸和自由的丝、苏氨酸对植物细胞膜上水透性有增强作用,如1.5mM N-磷酰化丝氨酸可使洋葱细胞质壁复原时间缩短一半以上。

Results In animal study, lumbricus could inhibit the growth of staphylococci, bacillus coli and bacillus aeruginosus. The time of wound healing in experimental group was 4 days shorter than that in control group. At 4d and 7 day the numbers of the capillary, blood vessel endodermis and desmohemoblast desmocyte and splitting epithelium of trial group were much more than those of control group. At 4d the trial group′s numbers of splitting mesenchymal cell were much more than that of control group. From 3d on the wound healing and granulation filling of experimental group were much quicker than those of control group.

结果 动物实验中,地龙能抑制金黄色葡萄球菌、大肠杆菌及绿脓杆菌的生长;实验组创面愈合时间较对照组提前了4天;第4、7天实验组动物创面的毛细血管数、血管腔内皮细胞数及间质成纤维细胞数均较对照组明显增多,上皮细胞分裂象也高于对照组,第4天的间皮细胞分裂象也高于对照组;自第3天始创面愈合及肉芽充填速度实验组明显快于对照组。

The research work involving the expression manner of doxA gene in S. lividans TK24 with plasmid pYG57 implied that the promoter PermE may control its expression constitutively, the time for maximum expression emerged from 48 to 60 h after inoculation and cultivation and the expression level was kept relatively stable, furthermore, the recombinant hydroxylase existed mostly in mycelium cell but little in broth.

而对Tm4(pYG57)菌株中doxA基因表达方式的研究表明, dd基因在 PermE启动子控制下可能是一种组成型控制表达,其表达量在接种后培养到 48-60 h左右最高并且维持相对稳定,并且表达的柔红霉素 Cl4羟化酶主要存在于菌丝体细胞内,很少分泌到细胞外。

The growth and proliferation of human prostate cancer PC-3 cell line could be inhibited by Ellagic Acid and its degree of inhibiting depended on its concentration and the time.

结论○1鞣花酸对人前列腺癌细胞株PC-3的生长具有明显的生长抑制作用,存在剂量和时间依赖关系。

At the same time, more and more studies have suggested that epigenetic regulation may play important roles in cancer stem cell development.

与此同时,越来越多的研究表明,对于肿瘤干细胞的发生与功能维持,表观遗传学的调控机制可能发挥着极其重要的作用。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. Then 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. The n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

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