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However, it is not known how orthodontic force influences the function of cementoblast and what is the relationship between the function change of cementoblast with root resorption. So we studied the effect of mechanical stimuli on root resoption by examining the expression OPG and RANKL and exploring the related signal transduction pathway in cementoblast. Methods: Cementoblasts OCCM30 were cultured in DMEM with 10% FBS for 48 hours and starved in DMEM with no FBS for 24 hours, then subjected to mechanical strain by four-point bending system with tension and compression stress at 2000μstrain at 0.5Hz frequency. The flow cytometry was used to examine the cell cycle and proliferation activity after 3h, 6h, 12h, 24h loading respectively. OPG and RANKL mRNA were analysed with real time quantitive RT-PCR after the same loading period as FCM.

本课题利用四点弯曲力学加载系统，采用流式细胞术、实时荧光定量PCR、Western免疫印迹等方法，研究体外培养的成牙骨质细胞株OCCM-30在不同力学环境中增殖活性的变化，以及其表达骨保护素和破骨细胞分化因子的mRNA的变化，探讨应力刺激对成牙骨质细胞表达破骨细胞分化调控蛋白的影响，并通过对应力刺激下成牙骨质细胞分裂原活化蛋白激酶家族中的ERK1/2、P38MAPK活性的变化及其与OPG、RANKL基因表达变化关系的研究，对力学刺激调节破骨细胞分化调控蛋白的相关上游信号转导通路进行初步探讨。

No structural change was found in the 4th group. Conclusion The test prove that cholestasis would occure during TPN and become serious with time prolonging. Integrity and function of gallbladder-wall tissue cell could be defended and sustained by applying GLN, but there is no direct preventing action. There is apparent cholecy stokinetic and cholagogic fundations by applying CCK. But CCK would lose its function if gallbladder-wall was damaged. The test prove that TPN+GLN+CCK is the best way to prevent cholestasis during TPN.

TPN时间越长，胆汁淤积越重； GLN虽无直接收缩胆囊作用，但它可保护胆囊壁组织结构完整，而CCK虽有收缩胆囊、减轻胆汁瘀积作用，但晚期当胆囊壁组织结构破坏时，其作用减弱；联合应用GLN和CCK可能是预防TPN期间胆汁瘀积的一种方法。

In SMC group, relative stable of the both ends of fracture reduce unnessary repeated stimulation of injury, and at same time, axio-stress advantageous for preosteoblast and mesenchymal cell transmit to osteoblast and chondroblast. Material and design superiority of SMC form unique internal mechanical environment, and which benefit for bone healing. The study took deep investigation of SMC on fracture healing.

上述结果证实：SMC内固定可对骨折端施加主动的、持续的轴向应力刺激，形成沿长骨骨干轴向的成骨导向力值，配合环抱翼的把持作用，形成了区别于CP、IN的独特的稳定、有效的机械内环境，骨折端的相对稳定减少了不必要的损伤再刺激，同时持续轴向应力有利于成骨祖细胞、间充质细胞向成骨细胞和成软骨细胞转化、促进了成骨诱导过程的发生与发展，加快了骨折愈合的修复与重建。

Both UV and NIR lasers were collinearly focused into 2.0 atm Ar gas cell,when satisfying the conditions of space-time overlap,the full width at half maximum spectrum of UV could grow from 2.6 nm without interaction situation to 6.4 nm,a factor-of-2.5 improvement,which can provide a valid way for attaining sub-20fs ultrafast pulses in the range of UV.

将紫外激光与近红外激光共线聚焦注入2.0 atm氩气样品池中，在满足时空重合条件时，半高全宽的紫外频谱宽度可从无相互作用时的2.6 nm展宽至6.4 nm，增加2.5倍，为进一步获取该波段小于20fs超快脉冲提供一个重要的途径。

Exploiting the high temperature fluxion character of grate cooler clinker, the filling state during entire colling process can be determined. Then impact on the control model can be analyzed introducing by the fluctuating of the clinker's thinkness and diameter. a modeling method with three dimensions division is proposed. The Longbeige step-changing dichotomy is used to optimize the division time toward z direction. Then the mass balance and heat balance analysis for every cell derived from clinker's three dimentions division is presented. Finally, the clinker temperature field's unitary control model with deducing in x, y, z direction separately is constructed.

从篦冷机熟料的高温流动特性入手，确定熟料在流动过程中的填充状态，分析熟料厚度与颗粒直径波动对控制模型影响，提出对篦冷机熟料采用三维分割的思想进行建模；设计了基于龙贝格变步长的三维分割算法，对z方向的分割次数进行优化；分析流动状态下熟料微元的质量平衡与热平衡关系，分别沿x、y、z方向递推，建立篦冷机熟料整体控制模型。

The cavity in core zone gradually turned bigger with longer culture time, whose volume was more than half of the whole inner structure at cell contabescence phase.

随着培养时间的延长核心区内的空腔逐渐增大，生长衰亡期的细胞聚集体空腔占据二分之一体积还多。

At time of 5mm length of alabatrum, the miosis in microsporecyte and the sex differentiation was found, thus in female flowers, pollen mother cell showed a abnormal meiose then the pollen cells gradually disintegrated, final, contabescence had taken, and in male flowers, the stamen normally developed.

在幼蕾长到5mm左右，即花粉母细胞开始减数分裂时，出现花性别的分化，雌花中雄蕊花粉母细胞不能进行正常减数分裂，进而花粉细胞逐步解体而导致雄蕊败育；雄花中雄蕊发育正常至性成熟。

The determination of reducing sugar ,chitinase, soluble protein content of three cell inclusions to Hyphae within the cells and found that the different trend over time: the reducing sugar content of synergistic mixture so that in 25 h, the decline for the contrast of 18.91%; chitinase activity in between 5~20 h increased; soluble protein contently increased by the subsequent and decline in the short term.

测定菌丝细胞渗漏的还原糖、几丁质酶、可溶性蛋白等三种细胞内含物含量，发现其随时间变化趋势不同：增效组合使还原糖含量在25 h时，下降为对照的18.91%；几丁质酶活性在5~20 h之间增加；可溶性蛋白含量短期内上升随后下降。

When St Francis had received this revelation, he forthwith shut himself up in his cell, and, in great recollection of soul, prepared himself for the mystery which was to be revealed to him; and from that time forth he began to taste more frequently the sweetness of divine contemplation, by which he was sometimes so absorbed in God, that he was seen by his companions to be raised corporally above the ground, and rapt in prayer; and in these raptures were revealed to St Francis not only things present and future, but even the secret thoughts and desires of the brethren, as was experienced by Brother Leo, his companion in those days.

当圣方济各收到这个启示以后，他立刻把自己关在房子里，努力回忆，为显示给他的这个奥迹作准备；然后，他开始越来越频繁地尝到了冥想的甘甜，有时候他如此的专注于冥想上帝，他的同伴发现他在全神贯注地祷告时，身体被抬起；方济各不仅仅得到了关于现在和将来的启示，也知道了他弟兄们心里的秘密。 Leo弟兄后来告诉我们。

At the same time the cell cycles of each group were determined by means of flow cytometer.

采用RT-PCR技术检测神经干细胞分化过程中GSK-3β、CDK-5和PP2A的mRNA表达。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新