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Methods Adrenocorticotropic hormone level in the supernatants of AtT-20 cells from the pituitary gland tumor was detected by radioimmunoassay at the given time points following administrating reagents and effects of R--MeHA ACTH on the cell proliferation was observed.

方法以高表达组胺H3受体的垂体细胞瘤AtT-20作为观察系统，用放免分析法测定给予H3受体激动剂后各时间点细胞上清液中ACTH分泌量的变化，并观察药物对细胞增殖的影响。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

Annonaceous acetogenin could inhibit cell proliferation in a dose-dependent and time-dependent manner in Raji cells, and it may induce Raji cells apoptosis by up-regulating caspase-9 expression.

番荔枝内酯对体外人淋巴瘤Raji细胞的增殖有抑制作用，并可能通过线粒体途径促进其凋亡。

Antimonate calcium precipitates in cell wall of hyphae grown for 5h in pH 4. 0 medium appeared less than in pH 6. 8 medium. And it was proved that pH in medium gradually reduced with the cultivation time during cultivation of hyphae.

在pH 4.0介质中培养5h后的菌丝，顶端细胞壁的焦锑酸钙沉淀比pH 6.8介质中培养的菌丝明显减少，并证实菌丝在生长过程中培养介质pH随培养时间的延长而逐渐下降。

It is the first time that these phenomena were observed: Two nuclei are surrounded by plasma and locate at the central of the female gametophyte and the others nuclei are positioned at the fringe of the female gametophyte; Before the pollen tube enters the female gametophyte. the nuclei in chalazal end begin splitting of plasma and form multinuclear cell.4. The pollens of Gnetumc are spherical or applanate with single aperture. The ornamentation of exine is spine. The basis part of spine is lenience and the top part of spine is tip or obtuse sphere.

首次在买麻藤属植物的雌配子体中观察到2个游离核位于配子体的中央位置，且被一团原生质所包围的，其余的游离核位于边缘的现象以及花粉管进入雌配子体前，合点端的核已经发生胞质分裂形成多核细胞的现象 4、买麻藤的花粉近球形或扁平型，有单萌发孔，外壁表面具小刺状纹饰，小刺基部宽大，末端尖或钝圆。

AITL, diagnosed based on lymph-node biopsy, was characterized by damage to normal structure of lymphonodus, proliferation of immunoblastic cells, and arborescent supervascularization. Immunophenotypes were observed in mature peripheral T-cell lymphoma. CVP regimen was the most commonly used chemotherapy. Fifty-eight percent of patients had a good initial response to chemotherapy. The 3-year survival rate was 25%, with a median survival time of 20 months.

确诊依据淋巴结活检，病理组织学呈淋巴结结构破坏，免疫母细胞增生，树枝状血管增生的特点，免疫表型全部为成熟外周T细胞性。12例均用CVP为主的方案化疗，总有效率58%。3年生存率为25%，全组中位生存20个月。

In addition, high-resolution time-of-flight secondary ion mass spectrometry was used to study the chemical composition of various pyrite forms in the No.9 coal seam (St,d = 3.46%) from the Wuda Coalfield. These include bacteriogenic, framboidal, massive, cell-filling, fracture-filling, and nodular pyrites.

运用高分辨率飞行时间型二次离子质谱仪，研究了内蒙古乌达矿区高硫煤9煤层（ St，d=3.46%）中的菌落成因、莓球状、晶粒状、胞腔充填型、裂隙充填型、结核状黄铁矿的化学组成。

After treatment of benzamide which is an inhibitor of poly polymerase,β-galactosidase activity assays and PCR analysis were performed to test the deletion of LacZ gene and c-Ha-T24ras gene. The results showed that:(1)LacZ gene was deleted from both A13.4-NIH3T3 cells and B12.7-NIH3T3 cells, but neigher from A13.4-HeLa cells nor B12.7-HeLa cells. So it is possible that there is cell line specificity in gene deletion induced by BA.(2) In A13.4-NIH3T3 cells, LacZ gene and c-Ha-T24ras gene were completely deleted at the same time.(3) The transcription activity of exgenous DNA fragment may have same effect on its sensitivity to the deletion induced by BA.

用聚ADP核糖聚合酶的NAD位点抑制剂苯甲酰胺分别处理四种转化细胞后，检测细胞中整合的外源LacZ基因与c-Ha-T24ras基因的删除情况，结果如下：BA诱导的基因删除可发生于NIH3T3转化细胞系，但不能发生于HeLa转化细胞系；位于同一外源表达载体上的LacZ基因与c-Ha-T24ras基因的删除过程是同步的；外源整合DNA片段的转录强度可能直接影响其被BA诱导删除的敏感性。

And when they put you in that cell,and those bars slam home,that's when you know it's for feal.Old life blown away in the bink of an eye.Nothing left but all the time in the world to think about it.

他们把你丢进囚笼，关上铁门，那时你才感到这是真实的，瞬间过去的一切都离你而去，剩下的只有对过去的回忆。。。。。。

Austrian companies from the new development of large photovoltaic Binodal silicon thin-film solar cell conversion efficiency of 8%, to reach the international advanced level; At the same time, the battery components can be greatly reduced installation costs, lower system cost of power generation and thus avoid the crystalline silicon the production process of high energy consumption, more conducive to energy saving and environmental protection, applicable to vehicle battery charging station, residential roofs, walls and large-scale power plant construction and other fields, has a broad application space.

由新奥光伏公司研制的超大型双结硅基薄膜太阳能电池的转换效率为8%，达到国际先进水平；同时可大幅降低电池组件的安装成本，进而降低系统发电成本，避免了晶体硅生产过程中的高能耗，更有利于节能环保，可适用于车用充电站、住宅屋顶、建筑幕墙和大型电站等多个领域，具有广泛的应用空间。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。