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Abstract] objective to explore the effect of radiotherapy on the auxesis of hela cell in cervix carcinoma to offer the theory elements to identify whether or not the cervix carcinoma would be treated by radiotherapy with non-routine segmentation.methods after radiotherapy with different doses,the hela cells changes of potential doubling time by cytometry with trypanblau coloration were analysed,and the livability of clonogenic cell at the same time was detected by clone,and the two former were combined to aralyse the changes.results (1)tpot shortening was observed in hela cell after radiotherapy.

目的 探讨放射对宫颈癌hela细胞系再增殖的影响,为宫颈癌是否有必要实行非常规分割放疗提供一定的理论基础。方法在不同剂量的放射作用下,以台盼蓝染色细胞计数法分析hela在照射后不同时间点的潜在倍增时间的变化;以克隆形成实验检测其同期克隆源性细胞存活比率,并将两者结合分析。

The results indicate that, during the early storage period under short time treatment with heat air, the activities of amylase and polygalactouronase are effectively decreased; starch content reduction is inhibited; the degradation speed of pectin and the decrease speed of flesh hardness are slowed down, and the release rate of ethylene in fruit is decreased. During the later storage period, the decomposition rate of middle lamella and primary wall of the Li jujube fruit treated with heat air for a short time is delayed, exosmosis of cell contents is inhibited, and the cell structure is protected, so the storage time of Li jujube fruit is prolonged.

结果表明,梨枣采后贮前短时间热处理能有效地降低早期淀粉酶和多聚半乳糖醛酸酶活性,抑制淀粉水解,延缓果胶降解,降低乙烯释放量,延缓果肉硬度下降速度,后期能延缓果肉细胞中胶层和初生壁分解,阻止细胞内含物外渗,保护细胞的结构,从而延长了贮藏保鲜期。

Blood bank type the appliance divided by the blood or blood component in the blood bank 滤 to use amid the leuco - cell, and the aetioporphyrin 品 only be limited by sex usage, that product a time the availability to do away with in order not to leuco - cell, object is the leuco - cell the antibody to spark not the 溶 guts have fever the anteiso -, anaphylaxis, HLA the immune response of of same race isoquinoline ketone body and big and small cytidine taken by leuco - cell k virus k, T lymph cell leukemia the k virus k's infection, prevent hard-pressed synthesis 症 of person respiratory trees

血库型应用于血库滤除血液或血液成分中白细胞用,本品只限于一次性使用,该产品有效去除白细胞,目的是为了避免白细胞抗体引起的非溶血性发热反应、过敏反应、HLA同种异体免疫反应以及由白细胞携带的巨细胞病毒、T淋巴细胞白血病病毒的传染,防止成人呼吸窘迫综合症

Results: The proliferation of Kasumi-l cell was obviously inhibited by VPA on dose- and time-dependent manner. The IC50 was 2.33 mmol/L treated with VPA for 72 h. Incubation with VPA, the cell ratio at G0/C1 phase increased. VPA induced myeloid cell differentiation antigen CD11b to increase with a time-and dose-dependent way and caused apoptosis. Wright's staining and light microscope were used to show the morphology of partial differentiation and obvious apoptosis with 3 mmol/L VPA for 72 hours. The typical DNA ladder of apoptosis characteristic was observed with DNA gel electrophoresis.

结果:VPA能显著抑制Kasumi-1细胞的增殖,且呈剂量和时间依赖性,VPA处理Kasumi-1细胞72h时的半数抑制浓度(IC50)为2.33 mmol/L;VPA处理后,细胞周期检测G0/G1期细胞比例逐渐增高;VPA能诱导髓系分化抗原CD11b表达水平的阳性率升高,呈时间及剂量依赖性;VPA能诱导Kasumi-1细胞凋亡,Kasumi-l细胞经VPA3 mmol/L处理72h后,出现典型的凋亡细胞所具有的梯形DNA条带。

Results1. Content of arsenic in serum raises with dose adding, time of pouring medicine and time of blood sampling prolonging. Thin layer chromatography indicates that there is toadfish in medicine serum of Liu-Shen-Wan.2. The suppressive rate of medicine serum of Liu-Shen-Wan on HL-60 cell raises with adding of dose and time of pouring medicine and prolonging of blood sampling.3. Gimsa dying assay, in situ end-labeling method and flow cell machine all exhibits: HI-60 cells dealed with Liu-Shen-Wan medicine-serum present typical apoptosis morphology.

结果:1 血清砷含量随着给药剂量、给药天数增加和采血时间的延长而升高;薄层色谱表明六神丸含药血清中有蟾酥成分。2 六神丸含药血清对白血病细胞的抑制率,随给药剂量、天数增加和采血时间的延长而升高3 吉姆萨染色、流式细胞仪检测、原位末端标记检测均证明六神丸含药血清能诱导HL-60白血病细胞凋亡。

The relative cell groth rate of L929 cell represented the proliferation of cells on materials with respect to control over time. The result showed that cytotoxicity of thermo-sensitive cell culcure dish was none or little,which can be used for lab use.Additional cell culture was performed with two types of celland cell sheets detachment were observed by reducing the culture tempareture after the confluently growth of the two types of cells.

进一步利用两种不同的细胞永生化的成牙本质细胞系和人牙周膜细胞在接枝有PNIAPm的培养皿上进行培养,观察到细胞可以正常贴壁,生长并增殖,并且在形成连续的细胞层之后,通过降低温度,获得了完整的细胞层结构,结合前两部分实验结果,证实了利用等离子体预处理和紫外线照射接枝制备温敏性培养皿的可行性,为后续研究提供了实验基础。

Conclusions1、E_2 may induce the apoptosis and Fas,FasL expressions of Jurkat cell,its effect positively related to the dose and treating time of E_2.2、There is intrinsic co-expression of ERαand ERβon Jurkat cell,which can be upregulated by E_2,positively related to E_2 doses.3、E_2 may act as an apoptosis inducer of Jurkat cell via ERβ.4、E_2 can promote the transcriptive activity of NF-κB in Jurkat cell.The increased NF-κB activity may lead to increased apoptosis of Jurkat cell.

结论1、E_2可增加Jurkat细胞的凋亡,并增加Fas、FasL的蛋白表达,且其作用与E_2的剂量和处理时间呈正相关。2、Jurkat细胞上存在ERα、ERβ的表达,且E_2可上调这两种受体的表达,E_2剂量越大,ER表达量越高。3、E_2可能通过ERβ发挥其促进Jurkat细胞凋亡的作用。4、E_2能增加Jurkat细胞中NF-κB的转录活性,Jurkat细胞中NF-κB活性增加有促进凋亡的作用。

The area is small to the highest ( conversion efficiency 13 - 15%, some have achieved more than 20%) of monocrystalline silicon solar energy cell conversion efficiency, and output power is big, and stability is good, grows ( generally can reach more than 20 years ) service life, but the raw and processed material cost height, the weak light specific property is wronger, and production technology is complicated, hence the selling price is also higher; The polycrystalline silicon solar energy cell is basic identical with the function characteristic of monocrystalline silicon solar energy cell; The conversion efficiency of non- brilliant silicon solar cell than hangs down ( simples about 6 - 8%), the area is than greatly, but because of his raw and processed material cost than hanging down, can be easy to big area industrialize production, what will have hope to be used the generally big area to generate electricity from now on most will be this kind of application that battery is along with manufacture technique and new material, in a single day the practical package photoelectricity conversion efficiency of its big area achieves more than the 10-15%, and the price of every tile electric power-generating equipment is fallen 1 - 2 U.S.dollar of time, and just is enough to that the solar energy cell efficiency that big area the getting extensive use will constantly replace in now convention certainly special use and the laboratory of sources of energy s will high get many.

单晶硅太阳能电池转换效率最高(转换效率13~15%,有的已达到20%以上),面积小,输出功率大,稳定性好,使用寿命长(一般可达20年以上),但原材料成本较高,弱光特性较差,生产工艺复杂,故售价也较高;多晶硅太阳能电池与单晶硅太阳能电池的性能特点基本相同;非晶硅太阳能电池的转换效率较低(约6~8%左右),面积较大,但因其原材料成本较低,可便于大面积工业化生产,今后最有希望用于一般大面积发电的将是这种电池。随着制造技术和新材料的应用,一旦它的大面积实用组件光电转换效率达到10-15%以上,每瓦发电设备的价格降到1~2美元时,便足以大面积得到广泛应用并将不断取代现在常规能源。当然特殊用途和实验室中的太阳能电池效率要高得多。

Factors involved in the expression of IFN -αwere studied. These included multiplicity of infection, cell inoculation concertration and cell growth phase. The results show that lower mulitiplicity of infection (M01.4PFU/cell) is beneficial to IFN-α production.The optimal cell inoculation concentration was 〓 cells/flask. The high efficient replication of BmNPV-IFN-α and expression were also due to the proper inoculation time of BmNPV-IFN-α, which was at 48-60 house after cell passage.

研究结果表明,重组病毒感染复数的改变对α-干扰素的产量的影响是时限性的,在一定低感染复数时(Mo1.4PFU),α-干扰素的产量较高;对于重组病毒的复制,最适的细胞接种密度为3.6~7.0×〓细胞/瓶;在细胞指数生长前期(48~60小时)接种重组病毒,对于获得较高的重组病毒效价和α-干扰素的产量都是有利的。

The cell outline was collected with two-step expansion model of modified Snake,at the same time;greedy algorithm was applied to reduce the operative time of procedure.Upon the basis that the segmentation of wood cell intracavity and edge was finished,wood cell wall was identified.

利用改进的Snake两步膨胀模型对细胞轮廓进行提取,同时应用贪婪优化算法,减少程序运行时间,在完成对木材细胞内腔和边缘分割的基础上,完成对木材细胞壁的识别。

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