查询词典 time cell

To produce medicine serum of Liu-Shen-Wan with different dose, different time of pouring medicine, different time of blood sampling.2. To measure content of arsenic in serum with atomic absorption method, to qualitative analysis toadfish in medicine serum of Liu-Shen-Wan with thin layer chromatography.3. MTT and trypan blue assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells and to make certain the best scheme of making method of medicine serum of Liu-Shen-Wan.4. Gimsa dying assay observes morphology changes of apoptosis .In situ end-labeling method and flow cell machine quantificationally probes apoptosis phenomena.

1 分不同的给药剂量、给药天数和不同的采血时间制备含药血清。2 用原子吸收法测定血清砷含量；薄层色谱法定性检测六神丸含药血清中蟾酥成分。3 用MTT法、胎盘蓝染色法观察六神丸对HL-60细胞的增殖抑制作用，确定最佳血清制备方案。4 用吉姆萨染色法观察细胞凋亡的形态学变化，流式细胞仪、原位末端标记法定量检测细胞凋亡程度。

In this thesis, the heterojunction with intrinsic hydrogenated amorphous silicon thin layer ( heterojunction with intrinsic thin layer: HIT) solar cells are investigated. The solar cell structure of p/i/n are fabricated. The substrate is a typical 4-inch n-type wafer with (100) crystal orientation, 1~10 ohm-cm and about 525 μm thickness. The p and i a-Si:H layers are deposited by a 13.56 MHz plasma enhanced chemical vapor deposition. The i a-Si:H layers are especially deposited by pulse-wave modulation plasma technique with fixed plasma turn-off time toff = 10 ms and changed plasma turn-on time ton= 10 to 40 ms to selectively control the different silicon to hydrogen bonding configurations. The effect of various Si:H bonds on the performance of HIT solar cells are explored.

中文摘要本论文针对异质接面薄本质层(heterojunction with intrinsic thin layer： HIT)太阳电池进行研究，采用一般典型四英吋晶向为(100，厚度约为525 μm，电阻率约为 1~10 ohm-cmn型矽晶圆为基板，制作p/i/n太阳电池，p及i层薄膜以13.56 MHz电浆加强化学气相沈积，i层并采用脉波调变电浆技术，在固定电浆关闭时间toff = 10 ms及改变电浆开启时间由ton = 10 ms 至40 ms条件下制作具有不同矽氢键结微结构的氢化非晶矽薄膜以探讨不同矽氢键结对太阳电池性能的影响。

Lisya goes knitting an eye to stick series to want than common product result a few times tall, solved a tradition to stick film to stick 10-20 not only after minute dry the issue with effect short time is reached after attracting skin moisture issue, still can all night give skin filling water take a tonic to build up health, because from in the evening 22 when arrive the following day in the morning 5 when, it is the optimal time of skin rehabilitate, and the rehabilitate in Morpheus just is the most effective, at this moment the cell grows and repair is the most exuberant, the speed of segmentation should be compared control 8 times more quickly at ordinary times, skin is particularly strong to protecting what skin tastes to draw strength.

lisya去皱眼贴系列比普通的产品效果要高几十倍，不仅解决了传统贴膜贴10-20分钟后就干燥倒吸皮肤水分问题后及作用时间短的问题，还能整夜的给肌肤补水补养，因为从晚上22时到第二天早上5时，是皮肤修复的最佳时间，而睡眠中的修复才是最有效的，这时细胞生长和修复最旺盛，细胞分裂的速度要比平时快8倍左右，肌肤对护肤品的吸收力非凡强。

Methods: Between November 2003 and December 2005, 67 patients with renal cell carcinoma underwent retroperitoneal laparoscopic radical nephrectomy (34 patients, group A) and open radical nephrectomy (33 patients, group B). Operating time, blond loss, amount of postoperative drainage, time to ambulation, recovery of intestinal function after operation, the postoperative hospital stay, use of antalgesic and transfusion blood and plasma were compared between group A and group B, retrospectively. Results: In group A, the operations of 31 patients were successful and 3 cases failed, then conversed to open surgery. Firstly, one case were not decented the prerenal fascia and the other two cases occurred complications, one case was due to injurying genital gland vein, with blood loss of 500m1, another fatty renal capsula was too thick to explose the renal pedicle. The operations of group B were all successful, only one case was died of intracerebral hemorrhage after two monthes.

回顾性分析和比较后腹腔镜肾癌根治性切除术(Retroperitoneal laparoscopic radical nephrectomy， RLRN)34例与开放性肾癌根治性切除术(Open radical nephrectomy， ORN)33例，比较两种方法的手术时间、术中出血、术后引流、术后最早下床活动时间、肠功能恢复情况、术后住院时间、术后镇痛剂使用量、术中术后输血、血浆量、术后随访时间及手术效果结果：A组中1例因最初对解剖结构难于辨认，无法分离肾前筋膜至肾门而改行开放手术，1例因损伤精索静脉出血难以控制而改行开放手术，1例因肾周脂肪过多，无法将肾脏掀起显露肾蒂而行开放手术，31例手术成功。B组手术均获成功，1例术后2个月因大面积脑出血死亡，余均无瘤生存。

Functions: supplement collagenic fibre and effectively restore the damaged collagenic fibre. Provide the skin with moisture and nutrition. Remove the thin wrinkle and dry wrinkle around the eyes, delay consenescence of the eye skin. Stem cell activating factors can quickly activate the dermal cells in all layers, promote the renewal of aging cells and speed up the restoring and composing of collagenic fibre and elastic fiber to restore the aging skin. HA high-effectively supplements moisture that can be maintained for a long time. Grapeseed Oil, VitE and Green Tea Extracts Liquid feature in anti-oxidation, removal of free radicals, improvement of the skin metabolism and circulation, elimination of extra moisture and toxin, recovery of skin energy and vigor. Its long time use will make your skin tight and tender, presenting a new look full of vigorous charms.

产品功效：补充胶原蛋白，有效修复受损胶原纤维，为肌肤提供水分和营养，消减眼部细纹和干纹，延缓眼部肌肤的衰老；干细胞激活素能够快速激活皮肤各层细胞，促进衰老细胞的更新，加速胶原纤维和弹性纤维的修复与合成，修复老化受损肌肤； HA 强效补水，高效保湿；葡萄籽油、 VitE 、绿茶萃取液等抗氧化、清除自由基，改善肌肤的代谢和循环，排除多余水分和毒素，回复肌肤能量与活力，长期使用，将令眼部肌肤紧致细嫩，神采奕奕。

Through dynamic examination of electrolyses, this paper research on treatment efficiency of bipolar packed bed cell under different voltage, electrolyses time, PH value andThe experimental results show that the decompounding speed of phenol decrease with the electrolyze time increasing. With the cells voltage augment the decompounding rate of phenol increase also. But when the voltage exceed 10V, the increasing speed of decompounding rate decrease.

BPBC处理苯酚废水的实验研究结果表明，苯酚的降解速率与酚浓度呈一级反应关系，即随着实际电解时间的增加苯酚降解速率逐渐减小；随着槽电压的增大，苯酚的去除率也随之增大，但是槽电压超过10V后，苯酚去除率增加趋势减缓；在碱性条件下，pH值的变化对苯酚的去除率有一定的影响，随着pH值的增大，去除率逐渐的降低；空床流速过大时，苯酚去除率有一定的下降；投加电解质后，苯酚的去除率增加，而就投加NaCl和NaNO_3的情况下，投加等摩尔浓度的NaCl和NaNO_3，投加NaCl的效果要优于投加NaNO_3的效果；提高废水中的溶解氧有利于苯酚的去除。

We indicated from our test that the treatment concentration of 50 mg· L- 1 and exporsure time with 4h by the herbicide were believed to be the threshold concentration and threshold time for causing abnormal cell mitosis in Secale cereale and Hordeum vulgar, respectively.

人们对它的作用机理和作用部位[2]、它在土壤中吸收和降解[4]以及草甘磷对土壤微生物群落的影响作过一定的研究[5]，并已开始抗草甘磷转基

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示：(1)SOD活性在52.98~133.20 U·g-1·h-1之间，并在早期单胚形成后一直保持上升的趋势，表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关；(2)POD活性在0.05~0.50 U·mg-1·min-1之间，呈现出先下降后升高的趋势，在胚性愈伤组织中最高，而在后期单胚形成时降至最低，表明其与原胚团的分裂和分化呈正相关，但与PEMⅢ向后期单胚的发育呈负相关；(3)CAT活性在0.86~2.81 U·mg-1·min-1之间，表现出升-降-升的变化趋势，在早期单胚形成时升至最高，在早期子叶胚形成时降至最低，表明其与早期单胚的发育呈正相关，而与中期单胚和早期子叶胚的发育呈负相关。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。