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After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Sai Di is online and brand-new measure inside the server after be being opened formally, all Sai Di is online the new personality king that the player of zone server all can play by a definite date 6 days develops class game, game time is on July 10 morning 10: 00 inside measure open to come on July 14 afternoon 17: 00 ends, after the match ends, before 4 occupational grade ranks two states 3 player can get day day, empire rich and generous platinic money large award.

赛迪在线全新服务器内测正式开放后，所有赛迪在线专区服务器的玩家均可参加为期6天的新人王冲级比赛，比赛时间为7月10日上午10：00内测开启至7月14日下午17：00结束，比赛结束后，天朝、帝国两个国家4个职业的等级排名前三名的玩家可以领取丰厚的白金币大奖。

Objective To study the statement of immunoreaction of c Jun and functional recovery of spinal cord injury in rats after fetal spinal cord transplantation.Methods Experimental rats were divided into two groups:spinal cord hemisection injury combination with transplants of FSC to injured site group,and spinal cord injury that received gelfoam pledgets group.On the lst,3th,7th,14th and 28th day after operation,6 animals in each group at each time phase,were observed for their function recovery with the methods of praxiology and electro physiological examination,the animals were then sacrificed for the observation of the statement of c Jun reaction by immnochemistry method and computer image analysis system was used for quantitative analysis.

目的：研究大鼠胚胎脊髓移植后能否影响，c-Hun免疫反应的表达和脊髓损伤后大鼠功能的恢复，方法；将动物分为脊髓半切洞损伤加胚胎脊髓移植组和单纯脊髓板切洞损伤加明胶海绵填塞组，每个时相点每组6只动物，术只1，3，7，14和28天，应用行为学和电生理检查观察大鼠功能恢复情况，应用免疫细胞化学方法，c-Jun免疫反应的表达，采用计算机图像分析技术，进行定量分析。

I was preparing liquid bacteria medium, after the short-time CoQ10 group conversation, I was feeling a little fine, but in the afternoom as the fermentation liquid media was cooled after 121 degree centigrade's heated, when the pH electrode is press out in the force of high pressure, that means I should reprepare the bacteria medium again.

我还是象往常一样配制培养基，本来心情还是很好的，可是当发酵液快降温的时候，pH电极被压力压了出来，晕！

After 2, 4, 6, and 8 weeks of feeding, 2% fluorescein sodium was dropped into rat conjunctival saccus and breakup time of tear film was observed by cobalt blue light scanning under slit lamp microscope. Then the rats were executed after anesthesia.

分别饲养2，4，6，8周后，用2%荧光素钠滴入大鼠结膜囊内，瞬目后在裂隙灯显微镜下用钴蓝光扫描照射观测大鼠的泪膜破裂时间，然后麻醉下颈椎脱臼法处死大鼠。

In the meantime, our salesroom and after-sale service department have been completed in the outskirts of Beijing,which fills up the blank of the after-sale at the appointed time.

与此同时门市及售后服务部在京郊大地落成。届时填补了起重设备售后服务方面的空白。

When Bogs and two of his buddies came after him a week or so after the laundry incident ("I heard ya go broke in," Bogs said, according to Ernie, who was around at the time), Andy slugged it out with them.

在洗衣房事件的一周后，当鲍佳斯和他的两个兄弟又来骚扰他时

The first to the market to buy a 3.3 jin of Solen, the school had a fair balance, not lack of weight (easily took the hands of the scale, the hawkers did not lack the basic weight), rushed to the 168 before 4:30, LG said, Let me Inside, and so on, he went outside to see the 168 selling price of seafood, the young girl to go out at the same time we bought Solen to the woman outside the wash (later know her as the young girl close to the checkout She handed over the money), I married a curious look on the outside to wash her, did not expect to see them sent to wash and burn the whole process of their guests to the Solen Add Taipen together, and mix with the other, one by one After the water washed into another Dapen months, and then look at the list of weight, you remove a few scales of the two, this is the Solen-after guests, because our Solen she is, she did not see I have to stand behind LG, when we raised objections, she said the amount was more or less the same now that you are both 2.7, 6, are the two intestines, comrades, 3.3 Solen two of her final with water scale Only two were 2.7, we get to the stomach, how many ah!

第一次是在市场买了3.3斤的竹蛏，学校有一个公平的平衡，而不是缺乏重量（轻松了手中的规模，小贩都缺乏基本的重量），送往168前4： 30日， LG电子表示，让我内，因此，他走到外面看到168售价海鲜，年轻姑娘走出去的同时，我们买的女子竹外洗（后来知道她的作为年轻的姑娘接近结帐她交出钱），我嫁给了一个奇怪的外观外洗她，没想到看到他们送到清洗和烧伤的全过程，他们的客人的竹蛏购买Taipen在一起，与其他组合，一个又一个的水清洗后，到另一个Dapen个月，然后看看名单重量，您删除一些尺度的两个，这是竹蛏，后客人，因为她是我们的竹蛏，她没有看见我站在后面LG电子，当我们提出异议，她说，数额大致相同，你现在都7月2日， 6日，是两个肠子，好同志， 3.3竹两个她最后与水规模只有两个是2月7日，我们到达胃部，有多少啊！

The inhibition substances in Sorbus pohuashanensisHedl fruit not only restrained the seeds of wheat, cabbage from germinating but also restrained height and root growth. After the seeds of wheat, chinese cabbage had been soaked in the water extraction of pulp, seed and decoated seed for 8h, the seed germination rates were 92% and 84%, 89% and 65%, 94% and 78%, lower than the control, especially the water extract of decoated seed; and the root length and seedlings height were lower than the control, especially the water extract of pulp, and decreased in sequence of soaking time from short to long. So the simplest and effective method to eliminate pulp soluble inhibiting substances is to wash fruits by flowing tap water soon after collection.

在几种浸泡时间中，浸泡8小时的去皮种子浸泡液对白菜、小麦种子发芽率的抑制作用最为明显，其发芽率分别为65%、89%，但随着浸泡时间的延长而降低；浸泡8h的果肉浸泡液对白菜和小麦幼苗高生长和根伸长的抑制作用最为明显，种子和去皮种子浸泡液(8h)的抑制作用对小麦和白菜的抑制作用次之，随着浸泡时间的延长，果肉浸泡液的抑制作用逐渐减弱，因而采收下来的果实及时净种是清除果肉水溶性抑制物质简单有效的方法。

Results:(1) The conchosporangia of brown yellow mutant was smaller and mature time was 15 days later than the parents. Thallus grew slowly in early stage, but average daily growth rate reached (7.50±1.18) cm after blade length was over 60 cm.(2)The emerald conchocelis were easy to mature and had special developmental mode that the spherulocytes can directly develop into sporangial branchlets and conchosporangia without thick conchocelis stage. Emerald blades were low in RPE with just (6.4710±0.0184) mg/g dry mass. The thallus grew quickly, average daily growth rate reached (11.95±2.33) cm after blade length was over 60 cm.(3) The conchocelis filaments of breen mutant were thin and short. Thallus grew slowly and had low contents of three phycobiliprotenin and chlorophyl.

实验进行50d。结果：(1)褐黄色突变体藻蓝蛋白和别藻蓝蛋白含量低；孢子囊枝的细胞较小，且大量形成时间比亲本晚15d；幼苗培养初期日平均生长量仅为(1.22±0.28)cm，当叶片长到60cm左右时生长优势逐步凸显，日平均生长量可达(7.50±1.18)cm；(2)翠绿色丝状体容易成熟，发育方式特殊，营养藻丝不经过藻丝加粗阶段，直接由球形细胞发育成孢子囊枝和壳孢子囊；翠绿色叶状体藻红蛋白含量低，仅有(5.5130±1.0496)mg/g；叶状体生长快速，60cm长的藻体日平均生长量高达(11.95±2.33)cm；(3)褐绿色突变体藻蓝蛋白、别藻蓝蛋白和藻红蛋白这3种色素蛋白和叶绿素的含量均较低；藻丝细胞短且细，叶状体生长速度较慢。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。