查询词典 tightly

She closed her eyes tightly in a vain attempt to hold back the tears.

她紧闭双眼，却无法忍住眼泪。

Methods we build rat retinal cells and rgcs cultured in virto by high pressure.put 80 mmhg pressure to rgcs in close tightly culture bottle for four hours, then collect rgcs and check the sum of apoptosis cells by flow cytometry.put the pressure 80 mmhg to rgcs in close tightly culture bottle for four hours,then collect rgcs and look for the apoptotic gene by gene chip.

（1）在rgcs中加入dsx，加压培养后收集细胞，用流式细胞仪测定凋亡细胞百分率。（2）在rgcs中加入dsx，加压培养后收集细胞，用基因芯片寻找凋亡基因的表达。结果（1）在rgcs高压培养时，dsx组的凋亡百分率低于模型组和对照组。

How tightly you actually swaddle your baby will take some practice, as some babies like to be swaddled very tightly and others like it a bit looser.

如何紧紧你，其实你的襁褓婴儿，将采取一些做法，因为有些婴儿喜欢被swaddled非常严格和其他人一样，它有点宽松。

Objective To compare the variability of adverse effects between BD closed tightly type detaining venepuncture and ordinary scalp venepuncture,and investigate the clinical effects applying BD closed tightly type detaining venepuncture in plasmapheresis.

目的 比较血浆置换术中采用BD密闭式静脉留置针技术与常规静脉穿刺出现不良反应的差异性，探讨BD密闭式静脉留置针技术在血浆置换术中应用的临床效果。

In general, you should: 1 weather strip the entire door jamb; 2 apply one continuous strip along each side; 3 make sure the stripping meets tightly at the corners; and 4 use a thickness that, when the door closes, the stripping tightly presses between the door and the door jamb without making the door too hard to close.

在一般情况下，你应该：1）天气地带的整个门门框； 2）适用于每一个侧面沿带钢连续3）确保在弯道符合剥离紧密，以及4）使用厚度，当门关闭时，之间的门和门框的剥离紧紧压而不太难的大门关闭。

Ping teachers arms tightly to my shoulder, very hard to send a lower body forward, arms around my own level teachers waist slim, flat hand with the teachers abundant buttocks plump, stem strength in her Daoli pulls out inserts , flat-teacher fold it tightly with the inner wall of the Road, sets of line with my stems, small lips wreathe my stem tightly.

平老师紧紧搂着我的肩膀，用力向前挺送着下体，我一手搂着平老师苗条的腰肢，一手抱着平老师肥美的丰臀，茎用力在她的道里抽插，平老师那紧紧的带有褶皱的道内壁套撸着我的茎，小唇紧紧裹住我的茎。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

80 Individuals of ATA-A F2 plants were used for RAPD molecular mapping of the monoecious gene by bulk segregant analysis. 840 random primers were chosen for polymerase chain reaction amplification between monoecious pool and andromonoecious pool, the results showed that: primer S128 was tightly linked to the monoecious character, and primer S493 was tightly linked to the andromonoecious character.

以甜瓜单性花系ATA-A系与两性花品种YJX-1的杂交F2代的80个单株为材料，利用群分法进行单性花基因有关RAPD分子标记研究，筛选分析了840个10bp随机引物的PCR扩增谱带，结果表明，随机引物S128在1180bp，S493在930bp处产生与性别相关的特异性条带，S128的特异条带与单性花性状相关，S493的特异条带与两性花性状相关。

As a sophisticated real-time running system with character of being real-time and tightly engaged, the feasibility of its actualization is studied at first through the aspects of logic and physical enforcement. Based on the demonstration in the project target and the technologic support, the HILSS is completely constructed, which is a tightly coupled multi-processor system composed of a standard personal computer, a high-performance single chip microprocessor system and a fast-running floating point DSP system.

为了研制这样一个复杂的实时系统，本文首先针对其实时性要求严格、系统衔接紧密的基本特征，通过实时结构化方法对系统的逻辑可行性和物理实施可行性进行了判断，在从设计目标和国内现有技术支持两方面对系统可行性进行论证的基础上，成功研制出整套ECU硬件在环仿真系统。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。