查询词典 thymidine

Then, stavudine III-2 was prepared fromβ-thymidine through another route via three steps in 69% overall yield.

然后，我们研究了另一条以β-胸苷为起始原料合成司他夫定的路线III-2。

Seasonal variation of bacterioplankton growth rate was measured with 3H- thymidine tracing method in Gehu Lake, along with the measurement of sedimentation of detritus, content of organic matter and C/N ratio of particulate and sedimented matter.

应用氚化胸苷示踪法研究了滆湖浮游物与沉降物中细菌生长速率的动态变化，同时测定了滆湖有机碎屑沉降量、水体中有机物含量以及C／N的动态变化。

After 5 days of coculture, the percentage of CD4 CD25 T cells was detected by flow cytometry. T cell proliferation was assessed by ［3H］ thymidine incorporation using a liquid scintillation counter. The expression of Foxp3 in CD4 CD25 T cells was detected by reverse transcription polymerase chain reaction.

众多学者对其可能的作用机制进行了研究，目前认为可能通过与免疫细胞（如T细胞，树突状细胞，B细胞等）直接接触［3］或通过改变细胞因子分泌网络间接影响免疫细胞发挥效应有关，但确切机制仍不清楚。

METHODS:U937 cells were labeled with ［3H］ thymidine in the culture medium, test reagents were added into the cell suspension in 24-well plates. The counts*min-1 were counted in a liquid scintillation counter.

［3H］-TdR标记的U937细胞培养于24孔板，在高分子激肽原，激肽释放酶(kallikrein，KK)，因子Ⅻ和纤溶酶原的作用下，观察U937细胞的粘附情况。

2 BJP direct toxicity on TEC NRK52E cell were subcultured on a 24-well plate (3×10〓/ml/well) and exposed to BJP κ or BJP λ in concentration 1, 5, 10, 20mg/ml for up to 24h, then the media was changed, and 〓H-thymidine was added for incubation for 18h at 37℃, the corporation was terminated, Acid-insoluble DNA was precipitated by adding 5% trichloroacetic acid radioactivity in the extracts was determined in scintillation counter.

细胞增殖的影响：将TEC株NRK52E以3×10〓/ml/孔分别加入24孔培养板，分别加入BJPκ与BJPλ（浓度1、5、10、20mg/ml）共同培养，均设3个复孔，24小时后加入〓H-TdR（1μCi/孔）在5%CO〓37℃孵育18小时，终止反应，加胰酶脱壁半小时后移到普通干燥试管，多头细胞收集器抽滤，用5%三氯醋酸，无水乙醇分别冲洗，烤干，冷却后加入闪烁液，放入液体闪烁计数仪测定放射性。

RDSSF could increase the incorporation of 〓H-Thymidine into DNA of Africa green monkey kidney cells (Vero E6) within the range of 1ug/ml～6ug/ml, but beyond the concentration of 2ug/ml, there is no increase of biological activity. RDSSF is stable within a range of pH 5.0～9.0 and sensitive to proteinase K and temperature.

RDSSF在体外可显著提高非洲绿猴肾细胞DNA中〓H-TdR掺入量，RDSSF浓度在1ug/ml～6ug/ml时活性明显，浓度达到2ug/ml后，增加RDSSF含量其活性不再增加；该物质在pH5～9范围内生物活性稳定；对蛋白酶k敏感；不耐热。

Heating, ethanol precipitation, and ion-exchange chromatographies had been carried out to isolate the protein with specific stimulating activity from newborn calf liver, and [(3)H]thymidine deoxyribose/bromodeoxyuridine incorporation and carboxyfluorescein diacetate succinimidyl ester-based proliferation assay to determine the bioactivity in vitro and in vivo.

通过用加热、乙醇沉淀、离子交换层析等方法来分离新生小牛肝脏中含有特殊刺激活性的蛋白和用胸嘧啶脱氧核糖/溴脱氧尿苷联合羧基荧光素双乙酸钠琥珀酸酯为基础的扩增分析其体内和体外的生物活性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。