查询词典 thymidine

The frequency value in S phase is 4. 83 times of M phase, 2. 9 times of G1 phase, and 3. 41 times of unsynchronized cells. These results suggested that gene correction events were highly related to S phase. Then we compared the targeting efficacy for two groups of cells in S phase. One group was contact with thymidine and the other one went smoothly through S phase without replication blockage.

基于这种原因，我们对基因定点修复事件做了细胞周期定位分析，发现同步于S期的细胞群发生定点修复的效率明显高于其它各期以及非同步化的细胞，流式细胞仪检测S期定点修复效率为M期细胞的4.83倍，为G1期细胞的2.9倍，是未同步化细胞的3.41倍。

The thymidine kinas gene was cloned from human herpes simplex virus Ⅱ by PCR techniques; eukaryon expressing vector pcDNA3/tk and pcDNA3/angio/tk fusion gene was constructed by DNA recombination; The treating effects of human primary liver cancer strain SMMC-7721 in vitro and nude mouse model transplanted with human liver cancer in vivo are studied.

通过PCR技术，由人单纯疱疹病毒DNA克隆获得单纯疱疹病毒Ⅱ型胸苷激酶基因；通过基因工程技术，构建真核表达质粒pcDNA3/tk及融合基因真核表达质粒pcDNA3/angio/tk；研究其对人原发性肝癌SMMC-7721细胞株的体外杀伤作用及对人原发性肝癌裸小鼠移植瘤模型的治疗作用。

METHODS Upon the model of cultured rat FBs, C a2+ influx stimulated with Ang Ⅱ,CaN signaling pathway was blocked with c yclosporine Aand Ca2+ channel with verapamil, detecting the ac tivities of FBs CaN, mitogen activated protein kinase and protein kinas e C,［3H］-leucine(［3H］-Leu) and ［3H］-thymidine(［3H］-Td R) incorporation as the target to evaluate FBs proliferation.

方法以培养的FBs为模型，用Ang Ⅱ剌激FBs外Ca2+入流，环孢素A阻断CaN信号通路，维拉帕米阻断FBs钙通道，检测FBs CaN、丝裂素活化蛋白激酶、蛋白激酶C活性，用［3H］-亮氨酸及［3H］-胸腺嘧啶参入量作为反应FB s增殖的指标。

At first, the zidovudine III-1 and stavudine III-2 were prepared fromβ-thymidine.

首先，我们以β-胸苷为起始原料合成了齐多夫定III-1和司他夫定III-2。

The extract was further iaolated with LC and purified with preparative HPLC, and uridine, adenosine, and thymidine were obtained.

为了提高菌体中总核苷的提取率，比较了干法和湿法提取对RCEF260菌体中总核苷含量的差异。

In the chapter 4, the HPLC analyses of Uridine and Thymidine implanted by ions with high energy are done. The result shows that the dose effect curves of ions with high energy are almost the same to the dose effect curve of ions with low energy. At the beginning, the damage of implanted ions to the sample is large, with the dose increasing, the damage drops sharply.

第四章对高能离子辐照尿苷和胸苷做了HPLC分析，结果表明高能离子辐照胸苷和尿苷样品的剂量效应曲线和低能离子基本相同，都是在注入初始阶段对样品的损伤比较大，随着剂量的增大，损伤迅速减小。

Following 24hrs of culture, cell proliferation was assessed by tritiated thymidine incorporation.

材料与方法：利用IL-2依赖T细胞株CTLL-2来研究腺苷对T细胞相关的IL-2R信号传导的影响，首先采用TdR掺入法分析腺苷对CTLL-2接受IL-2刺激后的增殖反应可能产生的影响。

By incorporation of tritiated thymidine or 5-bromodeoxyuridine and using them as the markers, we found that the basal cells, Clara cells and type II alveolar epithelial cells in the pulmonary tissues may in some way behave like stem cells and can eventually differentiate into respiratory tract epithelium.

通过应用cells in induced group cultured for 2 weeks 标记的胸腺嘧啶(3H-TdR)或5-溴脱氧尿核苷的渗入进行标记，发现肺组织的基底细胞、Clara 细胞和肺泡II型上皮细胞可能具有干细胞特征，能最终分化形成气道上皮。

By incorporation of tritiated thymidine or 5-bromodeoxyuridine and using them as the markers, we found that the basal cells, Clara cells and type Ⅱ alveolar epithelial cells in the pulmonary tissues may in some way behave like stem cells and can eventually differentiate into respiratory tract epithelium.

通过应用cells in induced group cultured for 2 weeks von kossa staining）标记的胸腺嘧啶(3H-TdR或5-溴脱氧尿核苷的渗入进行标记，发现肺组织的基底细胞、clara细胞和肺泡Ⅱ型上皮细胞可能具有干细胞特征，能最终分化形成气道上皮。

The multiplication conditions of bone marrow stromal cells were respectively detected by fibroblast colony forming in vitro, tritiated thymidine {[3H]TdR} incorporation and MTT.

分别应用成纤维细胞体外集落形成法、[3H]TdR掺入法和四甲基偶氮唑盐法检测骨髓基质细胞的增殖情况。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。