查询词典 thymidine

Objective: To study the effects of herpes simplex virus thymidine kinase gene transduction on adenoid cystic carcinoma cells.

目的：检测单纯疱疹病毒胸苷激酶基因的转导对高转移性涎腺腺样囊性癌细胞生物学行为的影响。

In order to further explore the relationship between this effect and the synthesis of RNA and DNA in the neurons, an autoradiography of 3~H-uridine and 3~H-thymidine was used.

本文应用3~H-尿嘧啶核苷和3~H-胸腺嘧啶核苷放射自显影术，进一步研究该作用与神经元合成RNA和DNA的关系。

Methods: Cells which belonged to the G1-phase were sorted by Multiparameter flow cytometry according DNA diploidy in asynchronous MOLT-4 cell lines, cells were synchronized at the G1-S boundary by thymidine, and western blot analysis and multiparameter analysis of DNA versus protein content was performed to examine cyclin A、B1、D〓、Eexpression in sorted G1-phase MOLT-4 cells and synchronized by double thymidine block in G1/S phase.

以MOLT-4细胞为模型，应用流式细胞术分选出非同步化生长的G1/S转换期起始处的G1期细胞，用"双thymidine阻滞法"将细胞同步在G1/S转换期起始处的G1期，采用DNA/cyclins双参数分析法及免疫印记法，检测同步化及非同步化G1期细胞中cyclinA、B1、D〓、E的表达。

At embryonic day 14, MEFs were recovered from the embryos and cultured under the selection of HAT (0.016 mg of hypoxanthine/ml, 0.01 mmol/L aminopterin, 0.0048 mg of thymidine thymidine/ml), and the cell cycle, apoptosis, and the relative protein expressions were analyzed by flow cytometry and Western blot.

用不同剂量的电离辐射或紫外光分别照射ES或EF细胞，然后利用流式细胞仪检测该突变对小鼠ES细胞周期及对ES和EF细胞凋亡的影响，并利用Western blot检测相关蛋白的表达。

It showed that thymidine could have special effect on cells in that replication in cells suffering thymidine treatment slow down substantially but do not stop DNA synthesis. This phenominon of leakiness in replication blockage provided chances for oligonucleotide to mediate repair.

通过检测上述三种抑制剂作用于细胞后不同时间的细胞周期分布，发现thymidine在抑制DNA合成的同时，存在着缓慢的渗漏作用，即染色质DNA上复制叉缓慢移动。

Thymidine phosphorylase is one of the main angiogenic factors.

胸苷磷酸化酶(thymidine phosphorylase，TP)是功能最强的血管形成刺激因子之一。

In the first subculture, with the increase of cataractous degree, epithelial cells of the lens showed lower attachment ability to culture plates and decreased ability to incorporate 3H-thymidine into newly synthesized DNA, ie, decreased proliferative capacity.

在第一次次培养时，随著白内障程度的增加，水晶体上皮细胞对培养皿壁的黏贴能力及生长中利用培养基中3H-thymidine来合成DNA的能力都显得较差，亦即有较差的增生能力。

Reaction of 5'-O-p-methoxytrityl-thymidine with 1, 1'-carbonyl-diimidazole gave the 3'-O-carbonylimidazolide, which was condensed in high yield with 3'-O-protected thymidine to give a dithymidine carbonate. In the same way, we obtained the protected trithymidine dicarbonate. After deprotection, we obtained quantatively the trithymidine dicarbonate.By the same stratagy, we completed the synthesis of dithymidine sulfite.It was shown that allyl can be used as a good protection group for hydroxyl of ribose in the synthesis of oligonucleotides consisting in some unstable nucleotide analogues.

我们在第一部分工作的基础上，以烯丙基作为3'-OH的保护基合成了保护的三胸苷碳酸酯以及二胸苷亚硫酸酯，用我们所得的脱保护条件进行了脱保护研究，得到了三胸苷碳酸酯以及二胸苷亚硫酸酯，并研究了它们合成中的一些化学问题，结果表明：烯丙基能很好地运用于这两类寡核苷酸类似物的合成中，其脱保护条件为中性，不会影响碳酸酯键及亚硫酸酯键。

Thymidine analogs, including bromodeoxyuridine, chlorodeoxyuridine, iododeoxyuridine, and tritiated thymidine, label diiding cells by incorporating into DNA during S phase of cell diision and are widely employed to identify cells transplanted into the central nerous system.

中文摘要：胸苷类似物包括溴脱氧尿苷、氯脱氧尿苷、碘苷和氚标记的胸苷，它们可以在细胞分裂的S期整合入待标记的分裂细胞，因此，被广泛地用于鉴定中枢神经系统细胞移植。

Thus, cells produced by"double thymidine blocks"are not experimental models for analysis of normal cell cylce.

用双thymidine阻滞干预的方法获得的同步化细胞并不是分析正常细胞周期的理想实验模型。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。