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The cell cycle and apoptosis and the expression of P-pg, bcl-2, P53 in the MG63 and MG63/R cell lines were analyzed by flow cytometry. Results The resistance index of the MG63/R cells to cisplatin was 83.557±4.841. The cells also had resistance to doxorubicin, vincristin, methotrexate and cyclophosphamide. Disordered structure of the MG63/R cells was observed through microscopy. The cells appeared in triangle, polygon and polynucleation. The increase of granular endoplasmic reticulums and apophyses was observed through transmission electron microscopy. The proliferation ability of MG63/R increased significantly, with a low apoptosis index.

结果 经顺铂186d的诱导，建立了MG63/R，其对顺铂的耐药指数为83.557±4.841，对阿霉素、长春新碱、氨甲基蝶吟、环磷酰氨亦产生不同程度的交叉耐药；光镜观察可见MG63/R细胞排列不规则，形态呈三角形、多角形及多核现象；透射电镜显示MG63/R细胞表面突起增加，粗面内质网丰富；细胞周期分析显示细胞增殖能力明显增加而细胞凋亡明显降低；MG63/R细胞 P-pg、bcl-2阳性表达较MG63细胞明显增加，P53表达则明显降低。

This is commonly a xenon lamp, in which electricity is arced through a tube filled with the gas. In a standard 35mm projector, the image is projected through the film and on to the screen using a series of lenses.

在氙气灯内，电流通过一个充满气体的显像管，弯曲成弧形。35毫米标准放映机通过一组透镜将胶片上的图像投影到银屏上。

In 2003, fate brought Jukai and Don together. The two were able to interact and interchange ideas and works, through the help of Coolmack Net. Not only were the two of them admiring each other's work, but they also discovered that they shared much similarity in the usage and creativity of the Adobe Illustrator. That was where it all started. In order for them to communicate and share their ideas much effectively, they discussed the possibility of setting up a website together. Then came the creation of their VI – Djebala. In the very beginning, Djebala was only meant to use for identifying the creation of Jukai and Don. However, it stormed the virtual design community with awes over a very short period of time and created itself a band of the vivid followers. Through these unexpected success and events, as well as all the interactions and support, Djebala grew stronger and gradually evolved into a complete "Graphic Design and Creativity Workgroup." Now, Djebala is not just aiming to create a website, it has grown much larger and greater goals.

秉持著这样的想法，2003年底某个偶然的机会，Jukai与小董两人在台湾一个著名的网路设计论坛「舞动影像」上进行了交流，其中除了彼此欣赏对方的作品外，发现在使用Adobe Illustrator软体绘制向量插图上，两人也有许多共同的理念，为了进一步提高两人作品在网路上提供浏览的方便性，於是逐步思考架设一个「联合个人作品网站」的可能，进而共同设计开发出一个VI─「djebala 激芭乐」，来作为此网站的整体视觉规划识别，真可谓无心插柳柳成阴，一路走来，受到了各种刺激与支持、有志之士的加入与合作，激芭乐渐渐演变成为一个完整的「图像设计创作团队」，凭藉著网路上连结起的同伴合作与交流，持续精进成长，朝向了一个远远比架设网站更大规模的目标迈进！

I bet most of you have painted a window with the sun shining through, or beams of light shining through the canopies in a forest.

我敢说你画过，一扇透过太阳光辉的窗户，或者是透过森林中的檐篷闪亮的光束。

Sales To achieve agreed monthly sales and average transaction value targets for area of responsibility To communicate sales targets and achievement against targets to the sales team on a daily basis To be proactive in identifying selling opportunities and putting in place initiatives that will help achieve and exceed the sales targets Product Knowledge To be able to provide the customer with product information that will enable them to make an informed decision about the item they wish to purchase To be able to communicate confidently the unique selling points and the features and benefits of the product To actively use this product knowledge to provide mix and match ideas for the customer and to up-sell to maximize the sales value Team Leadership To demonstrate and reinforce the Lane Crawford Vision And Values at all times and provide the necessary leadership to gain commitment from retail sales team To identify and execute ways to increase employee's loyalty, satisfaction and pride with the Lane Crawford experience To provide on job training to new hires to ensure they meet the required standards in all aspects of their job within the 3 month probation period To provide on job coaching to ensure that sales associates are able to consistently deliver and exceed the Lane Crawford service standards To train sales associates in product knowledge and mix and match To monitor and review performance of team with the DSM, identifying individuals who are ready for promotion or require performance improvement plans Manage performance of team on a daily basis and take appropriate action with those who fall below standard (in consultation with DSM/HR Department) Housekeeping To ensure daily housekeeping standards are maintained at all times through effective staff scheduling To maintain visual merchandise presentation in line with VM Standards Manual To prevent stock and pilferage by being alert and informing manager/security of potential issues To ensure cashiering function is performed to a high degree of accuracy To ensure stock is continually replenished to ensure a full range of items are available for the customer

顾客服务提供一致、友善和尽责的服务，确保连卡佛的服务标准在任何时间均得到贯彻执行在整个服务过程中积极主动，并提供独特服务，抓住每次机会，为顾客提供超越预期的购物体验向顾客和员工展现了解最新潮流与时尚的热切希望，并积极交流确保每次销售都有售后服务活动跟进，如预定、礼品包装、发送和投递、看管服务确保在零售区域随时有足够的人手提供预期标准的服务，随时提升日常业务水平收集顾客反馈信息并与经理/其他合适人员分享高效并有礼貌地处理和解决顾客投诉，令顾客满意销售达到责任区域内既定的月度销售额和平均交易值目标就销售目标和完成情况和销售团队进行日常沟通积极发现机会并主动创造，帮助达到和超越销售目标产品知识为顾客提供产品信息，使他们在充分了结的基础上对欲购商品做出购买决定能自信地表述产品的独特卖点、特征和优点积极利用产品知识，为顾客提供搭配意见，提升销售业绩，使销售额最大化团队领导随时展示并强调连卡佛理念和价值，展现必要的领导能力，以赢得零售团队的信任发现和执行能提高员工忠诚度、满意度和连卡佛体验自豪感的方法对新进员工的培训进行监督，确保其在3个月的试用期内在各方面均达到工作要求提供工作指导，确保销售助理能一贯执行并超越连卡佛服务标准对销售协理进行产品知识和搭配的培训与DSM一起监督和检查团队的业绩，确定哪些人适合提升，哪些人需要订立业绩改进计划对团队的工作进行日常管理，并对落后员工采取适当的措施内务处理通过有效的员工工作编排，时刻维护日常内务处理标准确保形象商品的展示符合VM标准手册要求保持警惕，就潜在问题通知经理/保安，防止积压和盗窃的发生确保收银职能的精确履行确保货源的持续补充，可随时向顾客提供全系列产品

This paper deploys research about the damnification identify, employ capacity and reinforce technology with reason of Double bend arch bridge through finding out disease position of construct and cause, colligating these result of the actual bridge detection and load test and analysis and research on the theoretics of damnification ,and so on,despending on a lots of measure and means ,finding out its present condition and checking structure internal force through choosing the proper mechanics model according as a lots of prototype information.

本题以安徽省省道S310上K139+000处的大店岗桥为实例，从检测入手，利用各种检测方法和手段，了解双曲拱桥的现行工况，并依据各类原型资料选择合适的力学模型进行结构内力验算，通过荷载试验、理论分析等方法，查明结构的病害部位和形成原因，综合实桥检测结果和荷载试验结果以及损伤理论的分析研究等等，展开对双曲拱类桥型的损伤鉴定、使用能力和合理加固技术方法的研究。

Connection with each species can come through the physical contact or seeing such species in nature, or through communing via intent of will.

与每一物种的连通可以通过物质层上的接触或在野外看到这些物种，或经由意志的意愿来交融。

Methods: The sample that pass through handled to be abstracted twice by acetonitrile, evaporate the organic phase to dryness following defecated through the Na2SO4, cleaned-up by norm alhexalle, then analysed by HPLC (C18 column) with a gradient system of methanol-water as a mobile phase.

经处理过的样品用乙腈提取两次，将乙腈过无水硫酸钠层后挥千乙腈，正己烷洗涤净化，经过净化后进行高效液相色谱仪测定（C18柱），本方法以甲醇、水为流动相梯度洗脱，柱温35℃，在波长275 nm处测定。

The feasibility of producing quality cold rolled sheets through deoxidizing and alloying with Fe-Al-Mn alloy and a process of producing low-carbon aluminium killed steel through deoxidizing and alloying are introduced.

介绍了攀钢用铝锰铁脱氧合金化生产高级别冷轧薄板用钢的可行性和脱氧合金化生产低碳铝镇静钢的工艺路线。

While this was often done with plaster, here two small holes have been drilled through the eyelids, so that dowels could be passed through to hold the inlay in place.

虽然这往往是与石膏，这里有两个小孔被钻通的眼皮，使钉可以通过在地方举行的嵌体。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。