查询词典 through and through

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

After going through the shrinking package line, the goods will be well-sealed into a see-through and nice pack that works against moisture, dust, and makes storage and transport jobs easier.

将包装好物品通过收缩机收缩后，可使被包装物外表密闭、透明美观。具有防潮、防尘功能，也便于产品的储藏和运输。

Then Saul said to his armor bearer, Draw your sword, and thrust me through with it, lest these uncircumcised come and thrust me through and abuse me.

31：4 就吩咐拿他兵器的人说，你拔出刀来，将我刺死，免得这些未受割礼的人来刺我，凌辱我。

Then Saul said to his armor-bearer,"Draw your sword, and thrust me through with it, lest these uncircumcised come and thrust me through, and mistreat me."

31：4 就吩咐拿他兵器的人说，你拔出刀来，将我刺死，免得那些未受割礼的人来刺我，凌辱我。

It is analyzed that the impacts of root weight on shear strength of unsaturated soil through direct shearing tests for unsaturated soil of different depth and various vegetation types which consist of bare soil and switch sorrel shrubbery under natural moisture contents and through the investigation of root system distributing along vertical profile.

通过对裸地、车桑子灌木林地不同深度土壤在天然含水量情况下的直剪试验，及对车桑子根系沿垂直方向的分布情况的调查，研究了含根量对非饱和土抗剪强度的影响。

It is analyzed that the impacts of root weight on shear strength of unsaturated soil through direct shearing tests for unsaturated soil of different depth and various vegetation types which consist of bare soil and switch sorrel shrubbery under natural moisture contents and through the investigation of root system distributing along vertical profile.

中文摘要：通过对裸地、车桑子灌木林地不同深度土壤在天然含水量情况下的直剪试验，及对车桑子根系沿垂直方向的分布情况的调查，研究了含根量对非饱和土抗剪强度的影响。

Some of famous scholars focused on evaluation model known as "Black and Scholes option evaluation model" which was established by Fisher Black and Myron Sholes in 1973. Option evaluation is no doubt very important to trader but how to make capital gain through put-call strategy is more concerned.We examined if we can make profit by the evaluation model of option and spot to research Taiwan option market merely through strategy implementation.

有的学者著重于评价模式之建立，如Fisher Black与Myron Scholes於1973年，提出所谓的&Black and Scholes选择权订价模型&交易人对於选择权评价的方式虽然重视，但是他们更想知道的事如何运用选择权的买卖策略来获取资本利得，但在相关研究上，对於此领域的研究却不是很完善，所以本文将对选择权与现货标的的评价方式透过策略的运用，重新评价，以台湾选择权市场为研究物件，仅就策略性操作是否有其获利模式进行研究。

As to the first research topic, we present a 'Noble Brand Positioning Four Master Phases Theory': Brand Space Theory, Brand Capital Theory, Brand Value Theory and Brand Circle Theory in advance. Following based on the Principal Component Analysis of statistical theory, we implemented the quantitative research in the 'Noble Brand Positioning Four Master Phases Theory'. We used the most famous fast food brand 'McDonald' s' as the sample space. The simulation test result shows that the Overall Index Explanatory Power is 83% under the Cronbath' s a is 0.88 through the quantitative verification. This result gives a strong support in defining the Noble Four Niche Phases Theory as the important strategic factor in establishing brand positioning index. After completing the quantitative research, the Principal Weight of each Sub-Niche is positive. This also implies that the Noble Brand Positioning Four master Phases Theory represents positive affection to brand positioning index. We also created the following Sub-Niches those are rarely mentioned in the past literatures. The Sub-Niches are:'Endless Running Strategy, Business Environment Strategy, Physical Capital Strategy, Brand Logo, Brand Music, Business Life Strategy, Personnel Capital Strategy, Life Cycle Strategy, Trust Mask Strategy, Re-Positioning Strategy, Word-Power Strategy, Globalization Strategy, Network Revolution Strategy and Green Brand Strategy'. The quantitative verification model can classify the responses of the sampling space into groups. After that, we used the quantitative verification process to classify the Target Consumer. And through the Principal Component Analysis, the group that owns the biggest average value consists of the Master Target Consumers for the business. This result would become the best suggestion for the business to adjust its strategies and to upgrade the productivity efficiency and create higher profit. As to the second research topic, we focused on finding the best corresponding characteristics attributes of the Brand Music for the Brand Log.

针对第一个研究主题，本文首先提出了一种创新性的品牌定位利基理论（A New Brand Positioning Niche Phase Theory）；品牌空间论，品牌资本论，品牌价值论与品牌循环论，接着本文以统计理论的主成份分析方法针对本文所提的创新性的品牌定位利基理论进行量化研究，本文以快餐业中知名品牌麦当劳作为样本空间，由量化验证的结果显示在信度分析Cronbatch's α为0.88之下其总指标解释力（Overall Index Explanatory Power）为83％，验证本文所提的创新性的品牌定位利基理论是构建品牌定位的重要策略因素，同时经由量化分析结果每项主成分权重值皆为正值更验证了本文所提的企业创新性的品牌定位利基理论与品牌定位指标呈现正相关：同时本文所提出次利基：'永续经营策略、企业环境策略、实体资本策略、企业生命策略、品牌标志策略、品牌音乐策略、人际资本策略、生命周期策略、信任标志策略、重新定位策略、文字威力策略、放眼全球策略、网络革命策略与绿色品牌策略'更是其它文献所未提及的：本文量化验证模式更可将样本空间中的responses分类群组，然后量化验证以界定目标市场，经由分析结果显示，其中具最大主成份百分位序均值者所属群组，即为企业中的主力消费群，提供企业决策者参考，进而调整其企业策略，而为企业提升生产效率创造更大的利润。

I drove through the cages lined boulevard and through the picturesque coastal road, less than half an hour you will reach Cavite history of the famous Kaweite than this card enjo and move on the island of Little Bay Resort and Leisure Park-an earth so you avoid trouble, easily leave the ideal place.

驱车穿过罗克斯林荫大道和经过风景如画的沿海路，不到半个小时您即可到达甲米地历史上著名的卡威特的比那卡烟城，继续向前就到了海岛小湾度假村和休闲公园-一处使您躲避尘世烦恼、轻松休假的理想之地。

Through acquaintance, while in Italy, with the work of Caravaggio and through contact with the Spaniard Jusepe de Ribera (1588-1656), he learned something of the potentialities of a very limited palette, black and neutrals, as is evident in many of his portraits, which are subtle harmonies of grays and blacks.

通过熟人，而在意大利，与工作卡拉瓦乔，并通过与西班牙的胡塞佩德ribera （ 1588年至1656年），他学到的东西的潜力非常有限的调色板，黑色和中立国的，这是显而易见的，在他的许多画像，这是微妙的和声的格雷斯和黑人。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。