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In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

We demonstrated the effect of the gelsemium total alkaloids of on blood and immune function of three different doses (0.15mg/kg,0.30mg/kg,1.5mg/kg) on mice and dieted with three GEB powers contents(1.5%,3%,6%) on chicken . The results showed as follow:1.The gelsemium total alkaloids of 0.15mg/kg,0.30mg/kg doses increased RBQHB, percentage of ANAE+ lymphocytes, phagocyty of peritoneal macrophage of mice.

本研究探讨了不同剂量(0.15mg／kg、0.30mg／kg、1.5mg／kg)的钩吻总碱稀释液对小鼠的血液学与免疫功能的影响，以及不同日粮添加水平(1.5％、3％、6％)的钩吻干粉对肉鸡血液学与免疫功能的影响，结果表明： 1、灌胃0.15mg／kg、0.30mg／kg剂量的钩吻液，能够提高小鼠红细胞数、血红蛋白含量、外周血ANAE~+淋巴细胞数、腹腔巨噬细胞吞噬指数及吞噬百分率。

Asynchronous loading of control parameters · Rapid (25ns per pixel) rotation, warping, panning, and scaling of images · Three-dimensional image addressing capability · General third-order polynomial transformations in two dimensions on-chip · Three-dimensional transformation of up to order 1.5 also supported · Flexible, user-congurable pixel datapath timing structure · Static convolutional ltering of up to 16 x 16 Pixel, 256 x 256 pixel or 256 x 256 x 256 pixel windows · User-selectable source image subpixel resolution of 2-8 to 2-16 · Pin-compatible upgrade to TMC2302 · 24-bit (optional 36-bit) positioning precision within the source image space, 48-bit internal precision · Low power CMOS process · Available in a 120-pin Plastic Pin Grid Array and 120-lead Metric Quad Flat Pack

1第1页，本页显示记录1-11，共11条记录分1页显示·异步控制参数加载·快速（25ns的每个像素）旋转，弯曲，平移，缩放和图像·三维图像处理能力·一般二维三阶多项式变换片·三高达1.5三维变换命令也支持·灵活，用户可配置的数据通道的静态时序结构·高达16 × 16像素滤波卷积像素，256 × 256像素或256 × 256 × 256像素（3通）窗口·用户可选的源图像亚像素分辨率的2-8 2-16·引脚兼容的升级TMC2302·24位（可选36位）的定位源图像的空间内，48位内部高精度·低功耗CMOS精密加工·提供120引脚塑料针栅阵列和120引脚公制四方扁平封装

I spent three days with him on three visits to Genoa.

在对热那亚的3次访问中，我和他相处了3天。

This thesis is consisted of two parts: Three-dimensional Geologic Information System design and Three-dimensional Visualization.

论文的第一部分是三维地质空间信息系统的总体设计，在这部分里，论文对三维信息系统的整体框架和各种功能进行了深入的探讨。

The main contributions are two-fold: Firstly, some theoretical analyses are carried out on trinocular rectification, including the relationship among the three rectified images and their three fundamental matrices, and an geometric interpretation of the 6 free parameters involved in the rectification process.

主要讨论两方面的工作。首先，对三视校正问题进行理论分析，给出了校正后图像的基本矩阵与其约束条件之间的关系，讨论了三视校正过程中的6个自由参数的几何含义。

Via the process of plane, three-dimensional and spatial information collecting, this study discusses whether the closure principle in Gestalt psychology can not only be applied to graphic design, but also the three-dimensional works and space management, in order to generate more multi innovative ideas.

本研究经由平面→立体→空间的资料收集与分析，探讨完形心理学中的闭锁性，除了运用於平面设计中，是否也可运用於立体作品、空间规划上以产生更多元性的创意发想。

Would it not make sense for the team, as one of the current quickest after the top three, to simply concentrate fully on 2009 and get the jump on the big three?

如果目前最快也只能排在三大车队之后，那么全神贯注备战2009年以挤入前三强，这样做对车队是否有意义呢？

There were but three disciples allowed to see the transfiguration, and those three entered the gloom of Gethsemane.

有些基督人喜欢常有特别的喜乐和启示；但是这并不是神的方法。

The duty of the drame and the music which was give by the subject was accomplished by three elements used in this dissertation as following:the musical theme"La Roi S'amuse"(from the opera"Rigoletto")was the center clue;the forming precess of the three main characters (Rigoletto、Duke and Gilda)was the major may to interprete the drama subject; meanwhile, their own Arias were used to rebuild the musical figures; and the chorus between each of them was the main means to put forward the plot of the drama.

本论文以歌剧《黎戈莱托》的主题"诅咒"作为主轴线，以歌剧中三个主人公(黎戈莱托、曼图阿公爵、黎戈莱托的女儿吉尔达)在歌剧中各自音乐形象的塑造过程作为对歌剧主题诠译的主要手段，同时又以他们各自在歌剧中的咏叹调作为塑造音乐形象的主要方式，以他们相互之间的合唱作为歌剧情节向前发展的重要方式，从而完成歌剧主题所赋予的戏剧任务和音乐任务。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新