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Factors that influence percentage of transformation including time of preculture, dipping and coculture, type and content of antibiotic, day of delayed selection culture, intension of selection were studied. The effective transformation system of "Starkrimson" was developed as follow: Leaves precultured in darkness for three days were dipped into agrobacterium suspension for three minutes, then were cocultured in darkness on MS medium with TDZ0.5mg/L and NAA 0.3mg/L for three days, and then cultured in darkness on MS medium with TDZ0.5mg/L NAA0.3mg/L and Cef 250mg/L.

通过对叶片预培养时间、侵染时间、共培养时间、抑菌素种类和浓度、延时筛选时间、不同选择压等遗传转化影响因素的研究,建立的'新红星'苹果遗传转化体系为:叶片黑暗预培养3天,经农杆菌侵染3分钟,在含TDZ0.5mg/L,NAA0.3mg/L的MS培养基上黑暗共培养3天,然后加250mg/L Cef黑暗维持培养3天,再进行Km12.5mg/L的选择压黑暗培养至抗性愈伤出现;转为光照条件1-2次的继代筛选,获得抗性芽后,进行扩繁。

The main organic acid was citric acid, followed by malic acid. Citric acid and tartaric acid levels increased during storage. Malic acid and quinic acid increased from stage one to stage two. Shikimic acid level increased from stage one to stage three, then decreased slightly. Acetic acid changed unremarkably. Succinic acid changed not significantly from stage one to stage two, then increased and it was the highest in the stage three. The level in stage four and stage five was lower than the level in stage three.

五九香梨果实中的主要有机酸是苹果酸和柠檬酸,整个贮藏期间,柠檬酸和酒石酸含量处于上升趋势,苹果酸和奎宁酸含量在第一、二月贮藏期间增加,而后开始下降;莽草酸含量在贮藏期第一到第三阶段上升,而后略下降;琥珀酸含量在第一到第二阶段变化不明显,接着有上升的趋势,在第三阶段含量达到最高,第四和第五阶段略低于第三阶段;乙酸含量变化不明显。

Except the foreword, the article is divided into three chapters:The first chapter is to make a systematical analysis on the institution of reorganization project, which can be further partitioned into three sections: the first section states the institution of reorganization, probes into the proposal of Project and principles of its institution, and claims that in order to attain a practical and enforceable project, the reorganization project should be set down mainly by reorganizer under the assistance of debtor; the second section explains the principles that should be complied with during the institution process, and deems honesty and rationality, thoroughness as the three most important principles; the third section set forth the content of reorganization project.

这一章共分三节:在公司重整计划的制定上,主张以重整人为主、有债务人予以协助来制定公司重整计划最为科学,这样制定的公司重整计划最切合实际,最易于执行。指出公司重整计划制定时应遵循公正合理、兼顾、效率三个原则。第三节讲述了公司重整计划的内容。公司重整计划草案应该包括以下四点核心内容:债权的分类方案;债权债务重整方案;重整企业的经营方案;重整计划的执行期限。

Methods:According to the principle of random design,the conditions would be consistent with the study of 90 cases of scapulohumeral periarthritis in patients with out-patient ratio of 1:1 were randomly divided into two groups,namely the "three-needle shoulder" with the temperature of acupuncture and traditional dialectical point acupuncture treatment,the treatment group:the "three-needle shoulder" with the warm acupuncture,three-needle shoulder(jianyu,before shoulder,jianzhen points) acupuncture,moxa check each 2cm,each pin of each combustion 2 day 1,10 times as a course of treatment,a total of 2 course of treatment.

根据区组随机化设计原则,将符合研究条件的90例门诊肩关节周围炎患者按1:1比例随机分为两组,分别给予温针灸&肩三针&和针刺&肩三针&治疗,治疗组:采用温针灸&肩三针&:先针刺肩三针,艾柱取每段2cm,每针每次燃烧2段,每天1次,10次为一疗程,共2个疗程。

For two Three-Columniation End Moment Equations at neighbor points, moved out sidesway , it is Three-span Moment Equation of frame that be relationship moments in three spans with load.

利用在连续两个结点上的三柱端弯矩方程,消去侧移分量产生刚架连续三跨的柱端弯矩与荷载关系的三跨弯矩方程。

There were no significant differences for the firing rates in the site of contralateral TNC neurons among during pre-CSD,CSD,and post-CSD (P>0.05).For flunarizine group,the firing rates in the site of ipsilateral TNC neurons during pre-CSD were higher as compared with during CSD(P<0.05).2.1 There were statistical differences on palasma levels of CGRP and SP among the three groups(P<0.05).The levels of CGRP and SP in CSD group were higher than control group(P<0.05).No significant differences on the levels of CGRP and SP in ipsilateral trigeminal ganglia were found among the three groups(P>0.05).2 The number of neurons with positive CGRP and SP immunoreactivity was statistically different in right-sided trigeminal ganglia among the three groups (P<0.05).The number in fight-sided trigeminal ganglia in CSD group was higher as compared with control group(P<0.05).The number in right-sided trigeminal ganglia was statistically higher than that in left-sided trigeminal ganglion in CSD group(P<0.05).3.1 Altered ReHo in ipsilateral pons and other brain regions response to pain such as basal nuclei,thalamus,cingulated gyms and prefrontal cortex was detected during the acute spontaneous attack as compared with during headache remission(P<0.05,corrected by Monte Carlo simulation). 2 Positive functional connectivity was detected between ipsilateral pons and other brain regions related to pain within pain state and within non-pain state (P<0.05,corrected by false discovery rate,FDR).Increased functional correlation between ipsilateral pons and other pain-related brain regions such as ipsilateral prefrontal cortex and contralateral subcallosal gyrus was detected during the acute spontaneous attack as compared with during headache remission(P<0.05,corrected by Monte Carlo simulation).

结果1。对照组未发现CSD;同侧TNC放电频率,CSD中>CSD后>CSD前P<0.05对侧TNC放电频率,CSD前、中、后无统计学差异(P>0.05氟桂利嗪组同侧TNC放电频率,CSD前>CSD中(P<0.05),CSD前与CSD后及CSD中与CSD后之间无统计学差异(P>0.05)。2.1关于放免测定,各组血浆CGRP、SP水平有统计学差异(P<0.05),CSD组高于对照组(P<0.05),CSD组与氟桂利嗪组、对照组与氟桂利嗪组之间均无统计学差异P>0.05各组之间同侧三叉神经节中CGRP、SP水平未见变化(P>0.05.2关于免疫组化研究,右侧三叉神经节CGRP、SP免疫阳性细胞数三组之间有统计学差异(P<0.05),多重两两比较结果CSD组大于对照组(P<0.05),CSD组与氟桂利嗪组之间、对照组与氟桂利嗪组之间无统计学差异P>0.05左侧三叉神经节CGRP、SP免疫阳性细胞数三组之间无统计学差异(P>0.05CSD组中右侧三叉神经节CGRP、SP免疫反应阳性细胞数大于左侧(P<0.05)。3.1局部一致性分析发现两组患者头痛疼痛状态较非疼痛状态脑活动发生变化的脑区有同侧脑桥以及其他疼痛相关脑区如基底节区、丘脑、扣带回、前额叶皮层等(P<0.05,蒙特卡罗模拟校正)。2功能连接分析发现疼痛状态与非疼痛状态下主要疼痛相关脑区均与同侧脑桥有功能联系P<0.05,false discovery rate,FDR校正疼痛状态与非疼痛状态比较,同侧前额叶皮层、对侧胼胝下回等疼痛相关脑区与同侧脑桥之间功能联系增强(P<0.05,蒙特卡罗模拟校正。

The mid-temperature vinyl sulphone reactive dyes are the most commonly used in most of domestic dyeing factories at present. Owing to three prime color dyes are the basis of match dyeing ,the stability of the three prime colors property determines the performance of the matched color ,so it is very important to select a team of low cost three prime color dyes with high light-perspiration fastness .

目前国内染厂大多数染色都采用中温型含乙烯砜活性基的染料,由于染料三原色是进行拼色染色的基础,三原色性能的稳定与否决定着拼出的色相的稳定性,选择一组具有较高耐光汗复合色牢度且拼色性能较好的低成本三原色具有重要意义。

So the three manuscripts in Marx\'s The Paris Manuscripts have supplely and profoundly exhibited the logical illustrations and the critiques on humanity by such three levels as the three-steps of capital legislation,the property rights(properties win over the real estates) as the natural rights and the liberal ideology of communism separately.

马克思《巴黎手稿》所包含的三部手稿,分别从资本立法的三个步骤、财产权作为自然权利、********的自由理念等三个方面对其进行了深入浅出的逻辑演示和人性论批判。

To obtain the tervalent fusion toxin gene, three toxin gene fragments from three species of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio mimicus were connected with the flexible linker using overlap extension PCR. The three toxin gene fragments respectively encode the mature proteins of the thermostable direct hemolysin of V. parahaemolyticus, the cytotoxin of V. vulnificus and the heat-labile hemolysin of V.

采用柔性Linker和重叠延伸PCR方法将三个毒素基因,即副溶血性弧菌的耐热型直接溶血素基因tdh、创伤弧菌的溶细胞毒素基因vvhA和拟态弧菌的热不稳定型溶血素基因vmhA的成熟蛋白基因片段进行串联,得到三联融合毒素基因tdh-vvhA-vmhA。

Three capsules or tablets of brewer's yeast three times per day for two weeks was reported to improve three cases of infectious diarrhea caused by Clostridium difficile . 24 Animal research has confirmed that brewer's yeast helps fight this unfriendly bacterium. 25 Note that real brewer's yeast is not identical to nutritional, or torula, yeast and that when asking for "brewer's yeast" in health food stores, people are often directed toward these other products.

三胶囊或啤酒酵母片两个星期,每日3次报告,以改善的三名艰难梭菌引起的感染性腹泻病例。24动物的研究已经证实,啤酒酵母协助打击这种不友善的细菌。25(请注意,真正的啤酒酵母不相同的营养,或torula,酵母和当为&啤酒酵母在保健食品商店&要求,人们往往直接针对这些其他产品。

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推荐网络例句

My first article for Screen Power Magazine, which is to this day is the official magazine of Jackie Chan, was a DVD review of the Hong Kong actioner Gen-X Cops, starring Nicholas Tse and Daniel Wu.

我的第一篇文章为屏幕力量杂志,在对这天是Jackie Chan 正式杂志,是香港actioner Gen X 警察的DVD 回顾,担任主角Nicholas Tse 和丹尼尔吴。

His lips grazed hirs, Now what are you going to do?

他的唇使吃草了 hirs,现在你将要做什麼?

I wasn't a huge drinker, but I enjoy it socially.

我不是一个贪杯的人,但在社交场合我会略微享受一点。