查询词典 thiol

The present invention relates to a process for rapid solution synthesis of a peptide, the process comprising repetitive cycles of steps-: a coupling step, using an excess of an activated carboxylic component to acylate an amino component, a quenching step in which a scavenger is used to remove residual activated carboxylic functions, wherein the scavenger may also be used for deprotection of the growing peptide, one or more aqueous extractions and optionally, a separate deprotection step, followed by one or more aqueous extractions, characterised in that the process comprises at least one step, referred to as step, in which an amine or a thiol comprising a free anion or a latent anion is used as a scavenger of residual activated carboxylic functions.

本发明涉及快速溶液合成肽的方法，该方法包括步骤-的重复循环：偶联步骤，使用过量的活化羧基成分酰化氨基成分，淬灭步骤，在该步骤中使用清除剂除去残余的活化羧基官能团，其中该清除剂还可以用于使生长中的肽去保护，一或多个水抽提和任选地，独立的去保护步骤，之后进行一或多次水抽提，其特征在于该方法包括至少一个步骤，称作步骤，在该步骤中包含自由阴离子或潜阴离子的胺或硫醇用作残余活化羧基官能团的清除剂。

At first, RNase A was denatured by urea and dithiothreitol. Effect of strong denaturant urea and DTT on enzyme activity and free thiol number was investigated.

首先利用脲和DTT作为变性剂，研究了脲和DTT浓度对RNase A的酶活和自由巯基的影响规律。

After developing 48 hours, hepatocytes enters 2.0 mM t-butyl hydroperxide or 2.0 mM diamide to induced cell blebbing. The samples collected at 0, 15, 30, 60 minutes to analyze cell about membrane protein thiol deletion; the leakage of lactate dehydrodenase; the change of glutathione; the lipid peroxidation; the variation of actin and tubulin, and use Confocal fluorescent microscope to observe the change of actin and tubulin in rat hepatocytes.

肝细胞在48小时培养后，分别加入2.0mM t-buty1 hydroperoxide或2.0 mM diamide诱发细胞膜小泡生成，并分别於0、15、30、60分钟取样，分析细胞膜硫醇流失程度；乳酸去氢□渗漏；□胱甘□浓度变化；脂质过氧化；肌动蛋白与管蛋白变化；并利用雷射共轭焦萤光显微镜观察肝细胞中肌动蛋白及管蛋白的变化。

The result indicates the t-BHP or diamide both cause the mass loss about the membrane protein thiol in hepatocytes, the fast oxidization about glutathione in hepatocytes, the leakage of lactate dehydrodenase, but the level of t-BHP is larger than of diamide. In the aspect of lipid peroxidation, only the t-BHP produces apparent change. As to actin and tubulin, they both find fall-off and present time-dependency effects. The result in Confocal fluorescent microscope also show these two cytoskeletal proteins are related to cell blebbing, but they don''t play the same role during the porcess. Tubulin may be more important than actin. If the study predispose dithiothreitol before adding t-BHP or diamide, then the damage by the diamide is repressed, but just partly protection for the damage by t-BHP.

结果显示t-BHP或diamide均可造成细胞膜蛋白硫醇大量流失与胞内GSH快速氧化，亦显著导致乳酸去氢□渗漏，但t-BHP诱发的渗漏程度大於diamide；在脂质过氧化方面，则仅t-BHP处理组产生明显的脂质氧化情形；至於肌动蛋白与管蛋白单体均可见减少且呈现时间依赖效应；在雷射共轭焦萤光显微镜观察中，亦显现这两种架构蛋白与细胞膜起泡有关，但两者在小泡生成的过程中扮演的角色并不完成相同，可能管蛋白较肌动蛋白重要；如果细胞在t-BHP或diamide加入前预先以dithiothreitol处理，则diamide 所造成的伤害显著的受到抑制，但DTT对t-BHP所诱发的伤害则仅有部分保护效果。

In Chapter four,we investigate the effect of different side groups,anchoring groups(dithiocarboxylate and thiol group),torsion angle and external electric field on the electronic transport properties of 4,4′-biphenyldithiolmolecular junction.

第四章中我们主要系统地研究了官能团、不同的连接方式、分子间的扭转角以及外电场对4，4\'-二巯基联苯分子的电输运性质的影响。

This function of PDIA3 in the ER is depended on the thiol-disulfide oxidoreductase which also plays an important role in gamete fusion.

目前研究发现位于精子表面的PDIA3的蛋白二硫键氧化还原酶活性在精卵融合中也发挥着重要的作用。

In one series of experiments, we observed the lengthening of the nine-carbon molecule nonyl thiol to form 10-carbon decanoic acid, a compound similar to the acids that drive metabolic reactions in living cells.

我们做过一系列实验，观察到九个碳的壬硫醇增长形成十个碳的癸酸，这个分子与细胞内驱动代谢反应的有机酸很类似。

This step produces a thiol ester. In the next step, methanolysis of the thiol ester is carried out in the presence of a basic catalyst.

该步骤产生硫羟酸酯，在下一步中，在碱性催化剂存在下进行硫羟酸酯的甲醇解。

Methods and Results— We synthesized the 3 components of a gene vector binding complex:(1) A poly allylamine bisphosphonate with latent thiol groups,(2) a poly ethyleneimine with pyridyldithio groups for amplification of attachment sites , and (3) a bifunctional (amine- and thiol-reactive) cross-linker with a labile ester bond.

方法和结果—我们综合了基因复合物载体的三种成分：(1)巯基聚丙烯胺二碳磷酸盐化合物，(2)能扩增结合位点聚乙烯亚胺-pyridyldithio复合物，(3)具有不稳定酯键的双功能交联剂。

The absorbency changes of both thiol and vinyl functional group were monitored independently by FTIR spectrometer during vinyl silazane photopolymerizing with ethanedithiol, Based on the results,the kenitics of reaction was studied and the convertion of both thiol and vinyl functional groups were investigated.

采用傅立叶变换红外光谱法跟踪巯基和乙烯基吸光度的变化，跟踪测定了光聚合反应过程中巯基和乙烯基的转化率，研究了乙二硫醇/乙烯基硅氮烷体系的光聚合反应动力学。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。