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Upstairs is right, not too tight, I also think that there should be no problem, there is a good attitude is the most important, do not be too nervous because of the impact of a child.

楼上说得对，不要太紧张，我也认为应该没有问题的，有一个良好心态是最重要的，不要因为过分紧张影响了孩子。

There is only too great a tendency in the best beliefs and practices to degenerate into the mechanical; and unless there were a succession of persons whose ever-recurring originality prevents the grounds of those beliefs and practices from becoming merely traditional, such dead matter would not resist the smallest shock from anything really alive, and there would be no reason why civilization should not die out, as in the Byzantine Empire.

在最好的信条和最好的做法中，向机械性退化的趋势只嫌太大了；除非有一连串的人以其不断产生的首创性阻止那些信条和做法的根据变成仅是传袭的，这样死的东西就不能抵抗任何真正活的东西的最小一点震撼，那就没有理由说文明为什么不会象在拜占庭帝国那样消亡下去了。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

Came to the lava cliffs fall zone, take the left side of the road directly in a group of birds where there are two columns below, the jump down (not dead, I am sleepy in this place as long as two hours), skip left after the first go, be reminded to observe, and now moving about in the magma, but can only be waist high magma, there is a need to see more of the terrain, do not waste HP, now jumped down, back to the left跳崖Department, went to is located in Magma, Fire Dragon Shou equipment can move around a half height of magma, first up, get a weapon, and then down, has been in the end, to continue filing filed walk inside the wind can be magic, the way to the red chest is a green precious stones, to increase the wind magic attack, and get back only to return to bandage things MM has emerged, but said a few left, and then returning to fight BOSS, the BOSS to attack his tail, the beginning of the head is of no use, finished BOSS, while red chest after using wind magic jump can be achieved

掉下悬崖来到了熔岩地带，直接走左边的路，在一群鸟的地方，下面有两根柱子处，往下跳(不会死的，我在这个地方困了2个小时之久)，跳下后先往左走，得到火龙守，现在可以在岩浆中走动了，但是只能是下半身高的岩浆，所以要多看地形，不要白白浪费HP，现在可以往下跳了，回到跳崖处往左，来到岩浆地处，装备火龙守，可在半身高岩浆里走动，先往上，拿到武器一把，再往下，一直到底，存档处存档继续往里走可得到风魔法，途中的红宝箱是绿宝石，增加风魔法攻击的，等回来时才能拿，返回事绷带MM又出现了，不过说了几句就走了，然后返回途中打BOSS，此BOSS要攻击他的尾巴，开始时打头是没有用的，打完BOSS，途中红宝箱利用跳后的风魔法可以取得

With a stone torrent run, it is not tired of living in a place away vagrants; a stone unprovoked manner, it is not in the form of well-being to come to you; a broken stone, it is not broken heart; a stone traveled back and forth, is it not pinpoint their location; hiding in the corner stone of inferiority is not always live, there are no tears stones, and for whom the tears flow?

一块石头随洪流跑了，是不是厌倦了一个地方的生活而去流浪；一块石头无端地来了，是不是以福祉的形式为你降临；一块石头碎了，是不是它的心碎了；一块石头来回辗转，是不是还没有找准自己的位置；躲在角落里的石头，是不是一直自卑地活着，石头有没有泪水，它的泪水为谁而流？

With a stone torrent run, it is not tired of living in a place away vagrants; a stone unprovoked manner, it is not in the form of well-being to come to you; a broken stone, it is not broken heart; a stone traveled back and forth, is it not pinpoint their location; hiding in the corner stone of inferiority is not always live, there are no tears stones, and for whom the tears flow?

一块石头随洪流跑了，是不是厌倦了一个地方的生活而去流浪；一块石头无端端来了，是不是以福祉的形式为你降临；一块石头碎了，是不是它的心碎了；一块石头来回辗转，是不是还没有找准自己的位置；躲在角落里的石头，是不是一直自卑地活着，石头有没有泪水，它的泪水为谁而流？

Fortunately, the prisoners did not wake up tomorrow a strong responsibility to hold possession, but after weighing the pros to thank him and let him have a reason to punish should have, both with each other when they meet again, said with regret, is willing to bear all of the results, This more or less eliminates the tubes out strong negative impact of the unit, Meng-Tsan Chiang had been staying during the observation accompanied by his side, and she said that the experience of his own daughter grow up, easy to deal with the problem is almost evening look, see Meng-Tsan Chiang not to return to En Yu's intention and asked if she could make Li Xing Yang months and come back to take in order to blot Fortunately, her childhood years to save some special scenarios, winter vacation has just come, I heard that Li Xing see his uncle to bring his mother, Yang months are excited, and bags large and small to pack a lot of things, is like home for Spring Festival is not the general, Qi-line anomalies that Meng-Tsan Chiang's trip goes well, hope she will take the opportunity to go out a chance to get a rest, if the Yang on call around, do not worry too much too tired, know that it is easy to Xi's idea, plus there are plum star escort, they did not say much else, then, and chose to send two men to the station early morning, because in a hurry to go to work, no more leave time to say good-bye to the unit.

幸运的是醒来后的犯人并未追究管明强的责任，痛定思痛之后反而感谢他，让他得到了理应该有的惩罚，两人再次见面时都互相表示着抱歉，愿意承担所有的结果，这多少消除了管明强在单位的负面影响，留守观察的期间孟灿一直陪在他身边，和她说自己女儿成长的经历，易夕看问题处理差不多了，见孟灿还没有要回恩宇的打算，便问她可否让李子星带杨晓月过来看看，以好在她童年的印迹里保存一些特别的情景，寒假刚刚来临，听说李子星叔叔要带自己见妈妈，杨晓月异常兴奋，大包小包收拾一大堆东西，好似春节也不回家过的般，杨启行得知孟灿此行异常顺利，希望她趁外出的机会好好休息休息，如果将杨晓月叫身边，也不要太操心太累，得知是易夕的主意，加上还有李子星护航，便没有多说别的话，择日清晨送两人去了车站，因赶时间上班，没多留点时间就道别去了单位。

Also, once the use of a product that is good, do not easily replaced, and some female friends very much like to try different brands, which can cause some problems, because each brand of skin care products technology system, composition, role There is a certain difference in the mechanism are, you must be a product for every change has a process of adaptation, some friends of skin resistance to strong, sometimes can not see what is wrong, there is a weak resistance to a friend for a product will be a problem .

还有，就是一旦使用某种产品好了，就不要轻易更换，有的女性朋友非常喜欢尝试不同的品牌，这就会引起一些问题，因为每一个品牌的护肤品的技术体系、成分、作用机理都存在一定的差别，你每换一种产品都要有个适应的过程，有的朋友皮肤抵抗力强，一时看不出什么不妥，有抵抗力弱的朋友一换产品就会出现问题。

Also, once the use of a product that is good, do not easily replaced, and some female friends very much like to try different brands, which can cause some problems, because each brand of skin care products technology system, composition, role There is a certain difference in the mechanism are, you must be a product for every change has a process of adaptation, some friends of skin resistance to strong, sometimes can not see what is wrong, there is a weak resistance to a friend for a product will be a problem .

还有，就是一旦使用某种产品好了，就不要轻易更换，有的女人朋友非常喜欢尝试不同的品牌，这就会引起一些问题，因为每一个牌子的护肤品的技术体系、成分、作用机理都存在一定的差别，你每换一种产品都要有个适应的过程，有的朋友皮肤抵抗力强，一时看不出什么不妥，有抵抗力弱的朋友一换产品就会出现问题。

Also, once the use of a product that is good, do not easily replaced, and some female friends very much like to try different brands, which can cause some problems, because each brand of skin care products technology system, composition, role There is a certain difference in the mechanism are, you must be a product for every change has a process of adaptation, some friends of skin resistance to strong, sometimes can not see what is wrong, there is a weak resistance to a friend for a product will be a problem .

还有，就是一旦使用某种产品好了，就不要轻易更换，有的女性朋友非常喜欢尝试不同的品牌，这就会引起一些问题，因为每一个品牌的护肤品的技术体系、成分、作用机理都存在一定的差别，你每换一种产品都要有个适应的过程，有的朋友皮肤抵抗力强，一时观不出什么不妥，有抵抗力弱的朋友一换产品就会出现问题。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。