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The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明:猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同,均属不稳定类酶蛋白;保守结构域氨基酸序列具有高度的同源性,均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序;均有信号肽,属分泌型蛋白质;属于跨膜的疏水性蛋白,均有5个跨膜结构域;无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件;三维结构预测都呈"V"字形;猪和牛HMGR基因的进化关系最近。

From the composite section, it can be found that some fossils'range zones are too short. Maybe it is caused by the fossils not preserved properly or the samples not collected comprehensively. Most fossils'first occurrence data are later than the ages which are defined by the MIDK4 composite section. It is probably caused by the Foraminifer zones in Tethyan- Himalaya which are not isochronous with other areas. It can also be recognized that there are two major extinction and revival events at the boundary of Cenomanian/Turonian and the boundary of Coniacian/ Santonian. They are coincident with the two Ocean Anoxic Events and can be considered as the typical response by creatures.

在复合剖面上可以发现:一些化石的延限非常短,这可能是由于化石保存不完整或者样品采集不全造成的;大部分化石的首现面年龄值都要晚于MIDK4数据库复合剖面给出的年龄值,这可能是由于特提斯喜马拉雅带与全球其他地区的有孔虫化石带不等时造成的;同时,可以在复合剖面上识别出在Cenomanian/Turonian界线附近和Coniacian/Santonian界线附近存在两次大规模的生物灭绝和复苏事件,这正好与两次大洋缺氧事件发生的时间一致,可以作为缺氧事件的生物标志。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明:①在适宜的浓度体外培养条件下,NSCs能形成典型的神经干细胞克隆球,Vimentin免疫荧光染色阳性,单靠丝裂原刺激或NSCs自我调节和分化诱导,不会产生典型的终末分化的成熟神经细胞;②海马源性的NSCs维持干细胞特性的时间更长,生物学特性更优;③传至第2~3代的NSCs尚未分化时移植最佳;④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞;⑤各种子代克隆球和细胞存在广泛的突触联系;⑥NSCs与OECs联合培养时,不需丝裂原刺激即能形成克隆球,获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞;⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化,但并不出现终末分化;⑧本研究培养条件不利于成纤维细胞、O4生长。

If you walk around in sweatpants and don't shave or shower, or look a bit sleepy, there's a high probability that there are going to be rumours out there that you're on drugs or starting some sort of spiral downhill.

比如你就穿着一条运动短裤,没刮脸没洗澡,或者是哪怕显示出一点困倦的样子,马上就会有传言说你吸毒或者说你到了低谷之类的。

There are racists, there is history distortion, there is ultranationalism in every country.

都有种族主义者,都存在歪曲历史的现象,也都有极端份子。

Gabriel Faure was deeply touched by Victor-Marie Hugo's poetry, and decided to creative his famous song 《Dream of love》: If there is a charming lawn, irrigated by the nature, and there are flowers in all seasons, there, people can pick up lilies, honeysuckles and jasmines, I will pave a path with these flowers, where you can step on it.......This leads to his philosophy: a man, full of love with his heart, will be blessed by the God, will feel happy forever.

作品描述:雨果的诗深深地打动了福雷去创作他的著名的艺术歌曲《爱之梦》:&假如有一片迷人的草地,天灌溉着它,在那里所有的季节都开着鲜花,在那里人们可以采下满捧百合花、金银花和茉莉花,我愿用这些花铺一条路,在那里你的脚可以踩踏……&从而引出他的哲理:一个充满爱心的的人会得到上帝的祝福,永远快乐。

There are a lot of shark attacks out there, but there were suggestions that it may not have been a shark, based on the lacerations.

那附近有很多地方都有鲨鱼攻击,但是从伤者的裂痕来看,他可能并不是被鲨鱼所伤。

Trust is often many "gentlemen's agreement", there is no legal text, there is the text there are too many loopholes.

而许多托管往往是&君子协定&,没有法律文本,有文本也存在很多漏洞。

There is also the use of "condoms" records, is the use of animal casings, said to be the sheep, pig bladder, said there are early "condom", but according to the real historical record, Western medieval palace in the use of the "condom" is The animal casings, there is a good listen to the name - Dutch cap.

也有使用&安全套&的记载,是使用动物的肠衣,有说是羊的,还有说猪膀胱是早期的&避孕套&,但据史料真实记载,西方中世纪宫廷里使用的&避孕套&就是动物的肠衣,还有个很好听的名字——&荷兰小帽&。

In all of Greek Literature, there are few places more famous than Pylos. And the location of Pylos was an ancient mystery, as an old riddle goes,"There is a Pylos in front of Pylos and there is yet another Pylos."

在所有希腊文献中,没有比皮勒斯更著名的地方了,它的具体位置是一个千古之谜,正如古老的谜语所说,&皮勒斯的前面有一个皮勒斯,另外还有一个皮勒斯。&

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Do you know, i need you to come back

你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。