查询词典 their eyes were watching god

It must be wrong,reading book can increase our knowledge, broaden our eyes, make us master special skills; If reading was uselessful, we'd lack of knowledges, couldn't explain some phenomena ago, such as thunder lightning , people unwisely regarded them as God's angering, now once own related knowledge, we can just phenomenalize them; Peasants planted their fields by raining throughly ago, whether rich harvest or not depended on God's face , now

这论断肯定是错的，读书能增长我们的知识，开阔我们的视野，使我们掌握专业技能；如若读书无用的话，我们就缺乏知识，就不能解释一些自然现象，比如以前的打雷闪电，人们愚昧地认为是天公发怒了，现在有了知识，就知道是自然现象；以前农民种地全靠天雨，丰收与否全靠老天的脸色，现在有了科学知识，我们可以人工增雨。

It must be wrong,reading book can increase our knowledge, broaden our eyes, make us master special skills; If reading was uselessful, we'd lack of knowledges, couldn't explain some phenomena ago, such as thunder lightning , people unwisely regarded them as God's angering, now once own related knowledge, we can just phenomenalize them; Peasants planted their fields by raining throughly ago, whether rich harvest or not depended on God's face , now we have science knowledges,we may artifical rainfall.

这论断肯定是错的，读书能增长我们的知识，开阔我们的视野，使我们掌握专业技能；如若读书无用的话，我们就缺乏知识，就不能解释一些自然现象，比如以前的打雷闪电，人们愚昧地认为是天公发怒了，现在有了知识，就知道是自然现象；以前农民种地全靠天雨，丰收与否全靠老天的脸色，现在有了科学知识，我们可以人工增雨。

It is certainly wrong,reading book can increase our knowledge, broaden our eyes, make us master special skills; If reading was uselessful, we'd lack of knowledges, couldn't explain some phenomena ago, such as thunder lightning , people unwisely regarded them as God's angering, now once own related knowledge, we can just phenomenalize them; Peasants planted their fields by raining throughly ago, whether rich harvest or not depended on God's face , now we have science knowledges,we may artifical rainfall.

这论断肯定是错的，读书能增长我们的知识，开阔我们的视野，使我们掌握专业技能；如若读书无用的话，我们就缺乏知识，就不能解释一些自然现象，比如以前的打雷闪电，人们愚昧地认为是天公发怒了，现在有了知识，就知道是自然现象；以前农民种地全靠天雨，丰收与否全靠老天的脸色，现在有了科学知识，我们可以人工增雨。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

In Alma it states,"Yea, he had been strengthening the armies of the Nephites, and erecting small forts, or places of resort: throwing up banks of earth round about to enclose his armies…the Nephites were taught…never to raise the sword except it were against an enemy… they had cast up dirtround to shield them from the arrows…the chief captains of the Lamanites were astonished exceedingly, because of the wisdom of the Nephites in preparing their places of security…they knew not that Moroni had fortified, or had built forts of security in all the land roundabout …the Lamanites could not get into their forts of security…because of the highness of the bank which had been thrown up, and the depth of the ditch which had been dug round about…they began to dig down their banks of earth…that they might have an equalchance to fight…instead of filling up their ditches by pulling down banks of earth, they were filled up in a measure with their dead…And caused them to erect fortifications that they should commence laboring in digging a ditch round about the land…And he caused that they should build a breastwork of timbers upon the inner bank of the ditch: and they did cast up dirt out of the ditch against the breastwork of timbers".

在阿尔玛书说：『他加强了尼腓人的军队，建造了小型堡垒，或凭藉处所；在周围筑起了堤岸来拱卫他的军队……尼腓人已被教导……除非抵抗敌人，决不举起剑来……他们在周围堆上了污泥来防避拉曼人的箭……这时拉曼人的总队长们非常的吃惊，由於尼腓人在准备他们的安全处所上所表现的智慧……因为他们不知摩罗乃已在周围各地的每一个城巿建筑了防御工事，或防御堡垒……拉曼人除了经由入囗处外，无法进入他们的防御堡垒，因为那土岸已堆得很高，那周围的壕沟已掘得很深……他们就开始去掘他们的土岸，使他们也能得到一条通往他们军队的小路，使他们能有一个均等的作战机会……非但没有推倒土岸来垫没他们的壕沟，反而将他们死者和伤者的身体垫进了不少……并吩咐他们建筑防御工事……他吩咐他们在濠沟的内堤上建造一道木头的胸墙；他们从濠沟中挖出污泥来，靠著木头胸墙堆起来

"Up to the limit" is an art work about time. On the surface, this art work tests people's tolerance of extreme tedium of endless routine labor and unbearably apathic physical space where one can hardly stay long. But the concept of time emerges during the process of close references with the formation of the work; moreover, a hidden craziness opposite to the apathy of the work sharply stabs into the viewers' eyes and further thrusts into their hearts; and it is exactly during the process of watching, that time settles down and achieves its existence.

极限》则是一组以时间为题的作品，表面看作品是在考验一种无穷尽的忍受，一种冷漠无比的枯燥，一种不能存留的短暂的物理空间，一种样式化的不断重复的工作，时间之概念恰在这一系列与作品形成密切相关的制作当中浮现出来，并以一种与作品的冷漠完全相反的隐藏起来的疯狂，尖锐地插进观看者的双眸，并延伸到其内心，时间恰好在这观赏的过程中得以落实，而成为一种实存。

Keto found that it has become crowded in her small space, with many people walking up and down it. Their eyes wide open, watching her. But she can't understand them.

刻托发现四周变得热闹起来，很多人走来走去，他们瞪大眼睛注视着她，可她读不懂他们的目光。

Through tears in their eyes, watching the rain and fog in the back of his father slightly bent and gray hair, how her son could not help, and lying on the wall and cried.

透过泪眼，看着雨雾中父亲微驼的背影和灰白的头发，儿子怎么也忍不住了，趴在墙上哭了起来。

Watching Glenn pet Nacho made me curious, how different the world is in their eyes.

看到Glenn蹲在地上逗Nacho，突然好奇心起，想看看他们眼中的世界到底有什么不同。

A boy stood out of the horizon where shadows of night had not conquered, watching also as the twin suns sinking, their images mirrored in his eyes, one orange-red and one yellow-white, cut in middle by a stread of clouds.

一个男孩独自站在尚未被暗影吞噬的地平线上，也在注视着沉落的夕阳，它们的影子映在他的眼中，一个橙红，一个黄白，萦绕着一丝云彩。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。