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Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Along with the mining industry declining, the settlement distribution, the population aging (in 96 year Tongshan Village old person ratio nearly 30%) with industrial reforming, under such environmental chane, Japanese Colonial Periodaround the time, the gold ore prosperous time, after the Recession of Taiwan in the recovering time historical vein, the substantive space, how does the social space's change live regarding the place maintains with is balanced; How between the reciprocity to achieve settlement old person each other's interaction and the familiar environment?

随著矿业的没落，聚落的分布，人口的老化(96年铜山里老人比率近30%)与产业的转型，在这样的环境变迁下，日治时期前后、金矿兴盛时期、光复时期之后的历史脉络里，实质空间、社会空间的改变对於地方之居住如何相维持与平衡；相互关系之间如何达到聚落老人彼此的互动与熟悉的居住环境？

The reliability of analysis result depends largely on the in-house experimental conditions. Unlike the previous analysis that only concerns the sole effect of permeable pressure, this article considers both permeable and confining pressure and deformation characteristics of concrete material under the interaction of them according to the real work state of construction, which may leads to the changing pattern of permeable rate as listed below: permeable rate always reaches its peak with the time process going on, than goes down and remains straight on some certain value; The PR will tend to decline while the confining pressure is increased, and the time taken before the peak will be extended as well; Under the constant permeable pressure and circularly changing confining pressure, the minus-exponential ascend and descend relationship could be applied between the PR and Time axes respectively with crannied and intact concrete samples, and the PR of crannied concrete is far more larger than that of intact concrete. The change pattern of concrete could also be mathematically analogized using the least square method.

室内的实验条件是研究成果可靠性的重要因素，而以往的研究都只考虑了渗透压单一作用，本文根据混凝土结构的实际工作状态，同时考虑了围压与渗透压共同作用下的混凝土材料的渗透性变化特征，得出了混凝土材料在围压作用与渗透压共同作用下的渗透率的变化规律：渗透率都是随时间推移先达到一个峰值，再降低并趋于平稳；渗透率会随围压的增大而减小，并且峰值的到达时间也会随之延长；在渗透压不变，围压升降循环的情况下，有裂缝、无裂缝混凝土的渗透率变化规律是随时间成负指数关系上升、下降，且有裂缝混凝土试件的渗透率远远高于无裂缝混凝土的渗透率，并对混凝土的变化规律运用最小二乘法进行数学拟合。

By time-varying filtering, we realize the signal selectivity and interference suppression, especially for the signals with time-varying power spectrum. It is shown herein that conventional eigenstructure based methods are lower dimensional special case of the time-frequency subspace fitting method. The closed-form array response estimation algorithms for blind source separation in wireless communication is also developed in the dissertation by exploiting the data structure of second-order statistics of the array output in the presence of multipath. The proposed method achieves array response estimation with little constraint on signal property and propagation environment such as scatters or angular spread. Moreover, the array utilized here can be of arbitrary geometry and even uncalibrated. Since the closed-form solution is obtained, no misconvergency may occur and large amount of computation can be saved. The equivalent feed model and corresponding optimization designation are proposed to realize the downlink multi-user selective transmission. By coherent and non-coherent superposition of the electromagnetical field, multiple beams can be formed using a single array, so that each co-channel user signal is transmitted in each beam and will not interfered with each other. The proposed system scheme implements the digital beamforming and the channel allocation in the base band, therefore is suitable for the utilization of the radio software technology. The feasibility and effectiveness of the proposed model are proved by the RF experiment.

在窄带条件下，GSC－SSF方法还可以提高阵列的虚拟孔径，从而提高估计的性能；可以证明，Cyclic DOA 和SC-SSF方法可看作该方法的特例或近似； 3、提出了二维GSC－SSF方法和宽带谱相关DOA矩阵方法，利用信号的循环平稳特性解决了宽带条件下二维 DOA 估计问题，并具有阵元数少、计算量较小的特点； 4、提出了时频子空间拟合和宽带时频DOA估计算法，将联合时频分析的理论和方法引入到阵列信号的空间谱估计研究中，利用时变滤波实现信号选择性测向和干扰抑制，特别适合于谱时变信号；可以证明传统特征结构法可看作是时频子空间拟合方法的特例； 5、提出了多用户环境下阵列响应的估计方法——CARE方法，该方法对信号结构和传播参数（主要是散射源分布与角度散布）的约束较少，适用于各种几何结构的阵列甚至未校准的阵列，能够直接得到阵列响应的闭式解，因此计算量小、稳健性好，不存在收敛问题； 6、提出了针对多用户下行选择性发送的阵列等效激励模型、优化设计方法及其系统实现方案，通过空间场的相干叠加与非相干叠加使得单一阵列能够形成多个波束，并保证各波束内的用户信号各不相同且相互干扰最小；其系统方案具有在基带实现数字波束成形、信道分配并便于软件无线电实现的特点，其有效性已得到外场实测数据的证实。

Thinking comprehensively of geological environment, ecological and nature environment, making surely the contents and emphasis of the environmental quantity assessment, establishing the index system of the environmental impact assessment and its ratings standard, studying the evaluation theories and its methods of the EQA, revealing time-space variety regulation of environmental quantity, studying the time-space distributes regulation of mineral pit water、waste heap eluviate water, tailingspile waste water and its heavy metals pollution and migration rules in surface water, groundwater, water department bottom mire, soil, applying division form R/ S analysis to research the development trend of heavy metals monitors data in water and soil, using mathematical statistics, gray theories, neural network, fuzzy evaluation, time order analysis to establish the forecast model of the EQA in mine geotechnical system.

综合考虑系统内地质环境、生态环境、自然环境，确定矿山岩土工程系统环境质量评价内容与重点，创建矿山岩土工程系统环境质量评价指标体系及其分级标准，研究矿山岩土工程系统环境质量评价理论和评价方法，揭示矿山岩土工程系统环境质量时空变化规律，研究矿坑水、废石场淋滤水、尾矿库废水时空分布规律及其引起的地表水、地下水、水系底泥、土壤等重金属污染及迁移规律，应用分形R／S分析研究水土监测时间序列发展趋势，再用数理统计、灰色理论、神经网络、模糊评价、时序分析等建立岩土工程系统环境质量预测模型。

According to by the time series estimate power spectral density\'s periodogram law principle,the research adopts five level of orbital power spectra which US Federal railway Administrative bureau FRA proposed;then,through the Fourier inverse transformation,the orbital not smooth time domain samples are obtained, which determine the track irregularity peak-to-peak value and the corresponding wave length; on the basis of the research,the influence of the wheel track dynamic contact force are considered.Based on the orbital roadbed coefficient\'s definition,computation model is established to solve the roadbed equispaced spring constant,and the roadbed uniform spring stiffness is obtained by using the numerical simulation method.Finally,based on the Matlab procedure platform,the computational procedure is established,and the load time interval caused by the moving train acts on the roadbed is obtained.

根据由时间序列估计功率谱密度的周期图法原理，采用美国联邦铁路管理局FRA提出的五级轨道功率谱，通过Fourier逆变换得到轨道不平顺的时域样本，确定了轨道不平顺的幅值及对应的波长，以此为基础考虑轮轨动态接触力的影响；基于轨道路基系数的定义建立了求解路基均布弹簧刚度的计算模型，采用数值模拟得到了路基均布弹簧刚度；最后，基于Matlab程序平台编制了计算程序，得到了运行列车作用到路基上的荷载时程。

After observing the behavior of Grus grus migration, the daily rhythm and time budget of various behaviors in the crane were revealed. The main behavior is feeding, accounting for 68.11% of the time budget, and the remaining time is spent resting 1.44%, alert 10.33%, walking 11.11%, preening 6.44%, and others 2.56%.

通过对灰鹤秋季迁徙期的行为进行研究得出了在这一时期灰鹤的日行为节律以及时间分配比例，一天中觅食占68.11%、静栖1.44%、警戒10.33%、游走11.11%、理羽6.44%、其他（包括打斗、追逐、鸣叫以及不可见的情况等）2.56%。

Sees only Moon crater which this at the beginning of war rests, from piece of on pale under black light and shade lamination dust, the slowly appearance, under the Moon small gravitation, true completely is being in the clouds quiet down, estimated needs at least for month-long the time, looked like the war the haze also must continue for a long time; Harbors the extremely serious mood, greatly leads several intimate assistants for doctor, passed through the thick dust dense fog, in basically cleans in the battlefield which finished to seek, the human brave warrior's skeleton already properly is processed early, but in the battlefield everywhere all has without enough time cleaning up each kind of weaponry, each kind of components which the guns, the bullet, the portable laser cannon and scatter, mixes with in the every large or small chippings, eliminates this also to have is wrecked innumerably, the kind of person robot makes threatening gestures to maintain each kind of death posture wreckage and the fragment, this gets down quietly the battlefield everywhere is filling the air the death breath, is constraining the people sensitive frail nerveDavid Dr.

只见这座大战初歇的月球环形山，正在从一片上淡下黑明暗分层的尘埃云中，慢慢的显露出来，在月球较小的引力作用下，真正的完全沉寂下来，估计至少需要一个月的时间，看来战争的阴霾还要持续许久；怀着极为沉重的心情，大为博士带领几位亲密的副手，穿过浓厚的尘埃迷雾，在基本打扫完毕的战场上寻找着，人类勇士的尸骸早已经被妥善处理，但是战场上到处都有来不及清理的各种武器装备，枪支、子弹、便携激光炮和散落的各种零件，夹杂在大大小小的碎石块中，除此还有无数被击毁的，类人机器人张牙舞爪保持着各种死亡姿态的残骸和碎片，这个沉寂下来的战场到处弥漫着死亡的气息，压抑着人们敏感脆弱的神经，大卫博士一行人，就这样巡视着并细心察看着这场战争的严重破坏。

Methods: By using JSB-1 with intramembranous antigen determinant as antibody, the positive rate as optimization index and MCF-7/A as model cells, cell pretreatment methods, such as the presence or Absence of FACS permeabilizing solution, the concentration of FACS permeabilizing solution, the time treating cells with FACS permeabilizing solution, the time incubating cells with JSB-1 working fluid, the time incubating cells with goat-anti-mouse IgG-FITC solution, and the conditions for keeping JSB-1 working fluid and cell numbers, were optimized.

采用抗原决定簇在细胞膜内的单抗JSB-1，以阳性百分比为优化指标，耐阿霉素人乳腺癌细胞系MCF-7/A为模型细胞，对FACS透化溶液的有无、FACS透化溶液的浓度、FACS透化溶液处理细胞的时间、鼠抗JSB-1抗体的孵育时间、羊抗鼠IgG-FITC抗体的孵育时间、JSB-1抗体工作液的保存条件、以及细胞数量进行优化。

At the same time it is know by analysis about matrix characteristic of the similar orthogonally that not only the characteristic value after the transformation is equal to the ones before the transformation and they are independence to time variable of the system, but also the eigenvectors of the formed system can showed the effect of the time variable on original system.

同时，根据矩阵正交相似的性质分析得知：不仅变换前后两个矩阵的特征值完全相同并与时间变量无关，而且时间变量对此系统的影响可以只由变换后系统的特征向量来描述，给出了非典型阻尼振动系统物理意义上的解释。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。