查询词典 the time changes

The study results showed that Pomacentridae and Labridae are the two most dominant families and the Pomacentrus coelestis is the most abundant species during day time. Species composition of coral reef fish assemblages at the four stations changed more or less each year. The Sorensen similarity values of the four stations fluctuated between 60-75%, fluctuation is mainly due to the changes in rare species.

其结果目前已知雀鲷科与隆头鱼科为此海域的优势鱼科，白天优势种则为变色雀鲷，4个测站都观察到每年鱼种的组成均略有改变，四测站Sorensen相似度约在60%~75%之间，差异主要来自稀有种。

Methods 120 SD rats were divided randomly into blank group, model group, Baoliezhi control group and Qianliening granule groups with different doses. The models were established with hypodermic injection of testosterone propionate at 4 mg/kg after orchiectomy, at the same time, these rats were gastric perfused with different drugs respectively for 30 days. The body weight, prostate weight, prostate index, morphological changes, the level of T and E2 were measured. Results The body weight in model group was significantly lower than that in control group (P.05), the prostate weight and index were obviously higher than that in control group (P.01). The prostate weight and index were decreased significantly in Qianliening granule groups with meddle and high doses (P.01), the level of testosterone was increased significantly and E2 was decreased obviously in Qianliening granule group with high doses.

将雄性SD大鼠60只，随机分成空白组、模型组、保列治治疗对照组、前列宁颗粒低、中、高剂量组，采用丙酸睾丸酮皮下注射引起大鼠前列腺增生模型，连续给药30d后，观察6组动物体重前列腺湿重前列腺指数前列腺组织形态血清睾酮雌二醇(E2)水平的变化结果模型组大鼠体重较正常组明显减轻(P.05)，而前列腺重量和前列腺指数明显升高(P.01)；前列宁颗粒中、高剂量组的前列腺重量和前列腺指数明显下降(P.01)；前列宁颗粒高剂量组可明显升高大鼠血清T水平，降低E2水平，使T/E2比例升高。

The difference of the world outlook between Faust and Gretchen reflected the changes of the ideas of the people in E urope in the 18th century and outstood the spirit of time of Faust.

她与 浮士德世界观的差异，反映了十八世纪的欧洲，人们思想观念的变化，突出了浮士德身上的时代精神。

The effects of climate warming on plant phonological changes from 1983 to 2004 in Guangdong are studied by use of the plant phenological data and temperature data of 10 Agrometeorological stations in Guangdong of the same time. The models for relationship between phenological periods and temperature are constructed. The responses of the phenological period to climate change are analyzed.

根据广东省气象观测资料和10个农业气象观测站的物候观测资料，分析了1982～2004年温度变化对广东省木本植物物候变化的影响，并建立了物候期差异与温度之间的关系模式，分析了当前气候增暖背景下物候期对温度变化的响应关系。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

The inhibition on the proliferation depended on the exposure time and dose.The cells showed morphological changes at the early stage of apoptosis under the light microscope:the shrunk and round cells;condensed cytoplasma and pycnosis nucleus and autophasosomes could be found under the transmission electron microscope.

在凋亡早期细胞形态学变化在光镜下可见细胞缩小变圆，在电镜上可见细胞质浓缩、核固缩及自噬溶酶体。4.0μmol/L As2O3作用72h后HT-29细胞凋亡率为25%。

The cation radical film exhibits an ageing effect, i.e. the changes in the physical or chemical properties of the film with time, following its deposition. It was found that because of a strong intermolecular interaction, ageing effect resulted in different aggregation states of cation radicals in the deposited film, and the film lost its reoxidation activity.

实验表明，由于分子间存在强烈的相互作用，陈化效应导致沉积膜中阳离子基具有多种不同的聚集状态，并导致沉积膜失去重新氧化的活性。

Methods:The conservative region of mTOR gene was inserted into pLXIN reversedly, then the vector was packaged in PT67 cells by transfection with lipofectamine and transfected to VSMC. The efficiency of antisense inhibition was verified,and the changes of p70S6k and 4E-BP1 were also determined at the same time. The proliferation activity was determined by flowcytometry and MTT.

采用脂质体介导转染包装细胞后构建mTOR的反义重组逆转录病毒载体(pLXIN-AmTOR)感染VSMC，检测mTOR及其下游底物包括真核细胞启动子4E结合蛋白1(4E-BP1)和核糖体蛋白S6激酶(p70S6k)的表达变化，以及VSMC细胞增殖周期和增殖活性的改变。

Results The PGE2 levels in the portal vein increased, while the PGE2 levels in peripheral blood had no changes significantly, the PGE2 levels in the tissues of the intestinal mucosa, placenta, amnion and amniotic cells were increased significantly; Ricinoleic acid stimulated the synthesis of PGE2 in the above tissues invitro, which had the positive correlations with the dose of ricinoleic acid and its lasting time.

结果 实验组大鼠门静脉PGE2水平升高，外周血PGE2水平无变化；实验组大鼠肠粘膜、胎盘、羊膜组织和羊膜细胞PGE2合成明显升高；蓖麻酸刺激肠粘膜、胎盘、羊膜等组织和细胞PGE2合成，其刺激合成能力与蓖麻酸浓度及其作用时间呈正相关。

"Playing soccer" the post-game title is "Cloe Ze and Borowski brings the attractive goal"(schone tore von klose und borowski), the article analyzes: In the German cup second round, Bayern has won "Bavaria Derby" the victory, Kelinsiman this competition changes four guard's tactics, this has also obtained the success, if is not the Bayern luck is unsatisfactory, they have the possibility to obtain more goals completely, but in Berry also celebrated his this season to present for the first time.

踢球者》赛后的标题则是&克洛泽和博罗夫斯基带来漂亮进球&（schone tore von klose und borowski），文章分析到：在德国杯第二轮，拜仁赢得了&巴伐利亚德比&的胜利，克林斯曼本场比赛变回了四后卫的战术，这也取得了成功，如果不是拜仁运气欠佳，他们完全有可能取得更多的进球，而里贝里也庆祝了他的本赛季首次亮相。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。