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Abstract] Objective To explore the effect of radiotherapy on the auxesis of Hela cell in cervix carcinoma to offer the theory elements to identify whether or not the cervix carcinoma would be treated by radiotherapy with non-routine segmentation.Methods After radiotherapy with different doses,the Hela cells changes of potential doubling time by cytometry with trypanblau coloration were analysed,and the livability of clonogenic cell at the same time was detected by clone,and the two former were combined to aralyse the changes.

目的 探讨放射对宫颈癌Hela细胞系再增殖的影响,宫颈癌是否有必要实行非常规分割放疗提供定的理论基础方法在不同剂量的放射作用下,以台盼蓝染色细胞计法分析Hela在照射后不同时间点的潜在倍增时间的变化;以克隆形成实验检测其同期克隆源性细胞存活比率,并将两者结合分析。

Abstract] objective to explore the effect of radiotherapy on the auxesis of hela cell in cervix carcinoma to offer the theory elements to identify whether or not the cervix carcinoma would be treated by radiotherapy with non-routine segmentation.methods after radiotherapy with different doses,the hela cells changes of potential doubling time by cytometry with trypanblau coloration were analysed,and the livability of clonogenic cell at the same time was detected by clone,and the two former were combined to aralyse the changes.results (1)tpot shortening was observed in hela cell after radiotherapy.

目的 探讨放射对宫颈癌hela细胞系再增殖的影响,为宫颈癌是否有必要实行非常规分割放疗提供一定的理论基础。方法在不同剂量的放射作用下,以台盼蓝染色细胞计数法分析hela在照射后不同时间点的潜在倍增时间的变化;以克隆形成实验检测其同期克隆源性细胞存活比率,并将两者结合分析。

Time series simulations are consistent with sediment records from lake bottom cores back to 100 years ago, and spatial distribution modeling of nutrient resources are agreement with changes in the geographical features. The work suggests that the simulations can catch basic characters of the basin nutrient changes, and provides a powerful tool to evaluate nutrient resources, sedimentations and distributions responding to historical changes in catchment environments.

模拟总氮和总磷浓度与太湖沉积钻孔沉积样推算的水体含量的数量级和变化范围相当,太湖流域的营养盐空间来源模拟也能够与流域自然地理变化吻合,表明模拟能够捕捉到流域营养盐历史变化特征,对湖泊营养物质来源、沉积和空间分布的流域环境历史变化响应具有良好模拟能力。

Objective: This paper aims to verify the curative effect that Jiusuyu cure the acute alcoholism disease by counting the changes of ebriety time, ebriety rate, sober-up time death rate, and discuss the effect of neurotransmitter and oxidative damage on acute alcoholism mice in brain tissue by examining the changes of SOD, MDA, DA, 5-HT, AchE in btain tissue.

目的:本研究旨在通过观察急性酒精中毒小鼠模型醉酒时间、醒酒时间、醉酒率、死亡率来验证酒速愈的药效,并通过检测脑组织中超氧化物歧化酶、丙二醛,纹状体多巴胺、5-羟色胺(5-HT)和海马胆碱脂酶指标的变化探讨该药对急性酒精中毒小鼠脑神经递质和氧化损伤的影响。

According to experimental results and etiopathogenisis and pathology of osteoporosis , and analyzing review investigation on hepcidin、associations of bone metabolism and iron metabolism and relations of ferri ion and calcium ion,we can make conclusions as follows: 1、We applied single manmade intervention approach to induce osteoporosis model in this study, there was no other influential factor. In the termination of test , rats bone density and bone histological anatomy indicated that osteoporosis model was creditable,meanwhile hepatic functional enzyme and hepatic microtome section demonstrated that hepatic function had noci-influence induced by medicinal herbs resource. Accordingly, we could believe that hepatic hepcidin gene expression and serum hepcidin contents can represent internal iron metabolism changes at selected test time. 2、In this investigation, different findings between test group and control group indicated that:according to changes of hepcidin gene expression measured by RT-PCR and changes of serum hepcidin contents determined by ELISA kit , there were correlations between hepcidin and rat osteoporosis model induced by retinoic acid. 3、Under the condition of this investigation,there was a interactive hypothesis as follows: Hepcidin iron metabolism←→ferriion←→calcium ion←→osteoporosis.

根据本次实验结果,对骨质疏松的病因病理的认识及铁代谢与铁调素、骨代谢与铁代谢关系、铁离子与钙离子的关系系列研究的回顾,经分析得出如下结论:1、本研究骨质疏松模型采用单一人工干预方法,没有其他影响因素;实验结束时大鼠骨密度、骨组织学检查表明骨质疏松形成,同时肝脏功能酶和HE切片显示肝脏功能未受药源性产生明显损伤性影响;因此,同期不同时间组大鼠肝脏铁调素基因表达、血清铁调素含量可以代表体内铁代谢不同时间的变化。2、在本研究中,模型组与对照组研究结果的差异表明:铁调素基因RT-PCR变化、血清铁调素ELISA测定含量变化与维甲酸制作的大鼠骨质疏松模型形成有实验相关性。3、在本研究平台下,形成了一个互相影响的假设图:铁调素←→铁代谢←→铁离子←→钙离子骨质疏松。

R. t routing changes is also studied, a transformation method for the calculation of sensitivity of sojourn time w. r. t routing changes is presented. The problem of calculating the performance sensitivity w. r. t buffer siaes in general queueing networks is also solved by using finite event time perturbation analysis.

对计算性能指标对路径概率灵敏性的转换法也进行了研究,提出了一种计算逗留时间对路径概率灵敏性的转换法,本文还应用事件时间摄动的有限摄动分析解决了一般排队网络的性能指标对缓冲器容量灵敏性的计算问题。

Methods①The animal model of braincontusion caused by free drop hammer was established.②The injuredtissue of rat brain were stained by TUNEL for apoptosis,immunohistochemistry for Caspase-3 and Feulgen"s for DNA. Image analysistechnique and the statistical method were employed to explorate thetemporal changes of injury time.③The DNA was extracted from ratcontusive tissue of brain and assayed by gel electrophoresis toinvestigate the relationship between the DNA fragmentation and injurytime.④One handred and seventeen cases of death from traumatic braininjury were retrospective researched to investigate the characteristicof TBI in forensic medicine.⑤The contusive tissue of human brain werestained by TUNEL, Caspase-3 immunohistochemistry and Feulgen"s methodsin the same way to analyze and disclose the linear relationship betweentemporal changes with injury time.

方法①建立大鼠自由落体打击脑损伤模型;②对不同损伤时间组的大鼠脑挫伤组织进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色,结合图像分析技术和统计学分析,探讨脑挫伤后神经细胞凋亡、DNA片段化和含量的时序性变化;③提取大鼠脑挫伤组织DNA进行琼脂糖凝胶电泳分析,观察DNA片段化随损伤时间的变化特点;④对117例颅脑损伤致死案例进行回顾性分析,探讨其法医学特点;⑤选择不同损伤时间的人脑挫伤组织同样进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色和分析,观察上述指标与损伤时间的关系。

Particularly the Cretaceous time period is a time of major changes in the Earth Systems demonstrated by major plate tectonic processes, as is the dispersal of Pangea supercontinent, plaeoclimate and paleoceanography changes.

Berriasian~Albian早期,延续时间超过30Ma;而在Thakkhola地区时代为Hauterivian晚期-Albian早期,延续时间约20Ma,在Zanskar地区火山岩屑砂岩仅仅出现在Albian期,延续时间小于10Ma。

Meanwhile, with the lapse of time, synchronization of different nations, the changes of immigration's identity, such a cultural system also undergoes lots of changes in both its heritage contents, channels and purposes, all of which resulted in changes of playwriting, composing, orchestration and performing rules.

同时,随着时代的推移、民族的融合、移民认同心理的变化,在传承内容、途径、目的等方面也有了很多变迁,从剧本、曲谱到乐器配置、演出制度都发生了变化。

There are one or two exceptions. There is, for instance, a kind of steel called invar (from"invariable") which does not change its dimensions as temperature changes; it is valuable for making pendulums , since, if the length of a pendulum changes, its time of vibration changes.

不过也有一两个例外,例如有一种钢叫做殷钢(从&invariable&一词而来),在温度变化时它的大小尺寸不变;这种钢对于制造钟摆很有价值,因为如果摆长改变,摆动的周期就改变。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。