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After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对,结果显示,PPV-SC1 NS1与其他的PPV NS1的同源性较高,仅存在个别的差异,分别是第39位A→G,第153位T→C,第175位A→G,第1117位A→C,第1535位A→C;同源搜索比较表明,PPV-SC1与PPV NS1同源性可达98%、99%,与其他的细小病毒NS1基因也存在很大的保守性;密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性;PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段,用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区,返回的结果并没有得到有显著意义的跨膜区的存在;根据基于motif数据库的结构域预测,PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP/GTP结合位点,该蛋白还存在16个蛋白激酶C磷酸化位点,21个酪蛋白激酶2磷酸化位点,3个cAMP-/cGMP依赖蛋白激酶磷酸化位点,PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域,推测NS1可能与POX_D5有类似的功能。

He wants to see the same faces, to read the same books, to do the same things day after day, month after month, while he is writing, so that nothing may break the illusion in which he is living--so that nothing may disturb or disquiet the mysterious nosings about, feelings round, darts, dashes, and sudden discoveries of that very shy and illusive spirit, the imagination.

他希望面对相同的人,读相同的的书,日复一日年复一年做着相同的事情,当他写作时,就没有任何事可以打破他生活在其中的幻觉了——打破或扰乱他对周围事物神秘的嗅觉与感知,那些急冲,猛击,然后突然发觉的微妙的幻想世界里的精神,那些想象。

Article 8 For national focal point shelter belt, and forest for special uses, the competent forestry authority of State Council shall produce opinions and report to the State Council for approval, then promulgate it; for local key shelter forest and forest for special use, the competent forestry authority of the people's government of a province, autonomous region or municipality directly under the central authority shall produce opinions and report to the people's government at the same level for approval, then promulgate it; for other shelter forest, timber forest or special-use forest as well as economic forest or fuel forest, the competent forestry authority of people's government at county level shall demarcate according to the state's relevant regulations on forest categorization and the arrangement and organization of the People's government at the same level, and report to the people's government at the same level for approval and promulgation.

第八条 国家重点防护林和特种用途林,由国务院林业主管部门提出意见,报国务院批准公布;地方重点防护林和特种用途林,由省、自治区、直辖市人民政府林业主管部门提出意见,报本级人民政府批准公布;其他防护林、用材林、特种用途林以及经济林、薪炭林,由县级人民政府林业主管部门根据国家关于林种划分的规定和本级人民政府的部署组织划定,报本级人民政府批准公布。

Such doubts havesitimulated the researchers'curiosity and start to explore the students' terms This research proceeds by observation interview test using learning sheets and etc The purpose is to explore students' terms of scientific concepts sources of terms connections between students' usage nomenclature and chooses of usage The results of the research are as follows: 1 There are five kinds of naming principles for students' terms of scientific concepts: appearance habit function replacing and sound Appearance principle contains three categories: color shape and partial construction; habit principle contains custom and ability; replacing principle contains reality and non-reality 2 Students' terms of scientific concepts come from two ways: self-acquisition and learning through senses The former way is only a bit more common than the latter 3 There are three different relationships between students' usage of terms and nomenclature: one both the same in usage and meaning; the other both different; another different usage but the same meaning Normally terms that students used are various from nomenclature in the aspects of usage and meaning only a few would match and even rare for "Synonyms" to occur 4 Alternative options for students' scientific terms are maintenance adaptation or co-existence with formal scientific terms The most common way of selecting usage of terms is to maintain Sometimes students would adapt themselves once learn of scientist's term However some believe that very odd chances for both could exist at the same time

这样的疑问深深引起研究者的好奇,著手探索学生科学概念所使用的语言。本研究透过观察、访谈、学习单施测等方法来进行,目的在探讨学童在科学概念上所使用之学生惯用语、学生惯用语来源、学生惯用语与科学家用语之关联性、学生惯用语选择。研究结果如下:一、学童在科学概念上所使用之学生惯用语的命名原则有五种:外型、习性、功能、藉代称呼及声音,其中外型原则有颜色、体型及部份构造三类目,习性原则有习惯及能力两类目,藉代称呼原则有实体及虚拟两类目。二、学童在科学概念上所使用之学生惯用语有两种来源,分别为自己称取和听来。学生惯用语来源较多为自己称取,透过其他方式听来少一点,但两种来源的比率差距甚小。三、学童在科学概念上所使用之学生惯用语和科学家用语的关系有三种,分别为用语意涵均相同、用语不同意涵相同和用语意涵均不同。学生惯用语通常和科学家的用语是不同的,且其意涵也不同,只有部份学生惯用语和科学家用语意涵均相同,至於学生惯用语和科学家用语不同但意涵相同的极少。四、学童的科学概念相关学生惯用语选择有三种,分别为维持学生惯用语、改变成科学家用语和科学家与学生惯用语皆可。学童的科学概念相关学生惯用语选择,以维持学生惯用语最多,而有些时候学生会在得知科学家的用语后改变成科学家用语,但认为科学家用语和自己使用的学生惯用语皆可的则很少。

Milling groove machine is used in processing weaving trade in " pin plate " use privately cut machine tool, belong to the modular machine tool, this machine tool have 4 works position and 8 milling heads, can at the same time process 8 workpieces, have higher processing efficiency and processing precision, and can at the same time still realize needle groove and circular arc groove same work position, it surmounted the shortcoming of that machining process complex, processing efficiency low and process precision do not be in height of the processing of pin plate beforetime, so it have better application prospect.

铣槽机是用于加工纺织业中&针板&的专用切削机床,属于典型的组合机床,该机床具有四工位、八铣头,能同时加工八个工件,具有较高的加工效率和加工精度,同时还能实现针槽与圆弧口槽同工位一次完成,克服了以前针板加工中加工工序复杂、加工效率低、加工精度不高的缺点,有较好的应用前景。

With the Securities and Exchange Stamp duty collections for the month to complete 3,230,000,000 yuan, compared to the same period last year, and to reduce 28,516,000,000 yuan, only one-tenth of the same period last year, the growth rate of 89.8 percent year-on-year decline in the same period last year by Speed (3320.9%) dropped 3410.7 points more than in January-July growth rate dropped 96.8 percentage points.

与证券交易印花税月份税税收完成323000.0万元,比去年同期,并减少285.160亿元,仅为去年同期的十分之一8年的增长去年的百分之89.8率按年跌幅在同一时期的速度(3320.9%)去年下跌3410.7点,比1-7月增幅下降了96.8个百分点。

The police found out during the investigation that quite a number of the "timely broadcasting systems" located in more than 10 provinces, autonomous regions and municipalities all over the countries used the loud-hail speakers of the same model produced by the same factory and more than half of the semiconductor power amplifier breadboards were assembled at the same location.

公安机关在调查中发现,遍布全国10余个省、自治区、直辖市的这些&定时播音装置&,其中相当一部分使用的竟是同一厂家、同一型号的扩音小喇叭,大部分半导体功放线路板也出自同一个组装点。

We find that model LBGs can have the same Two Point Correlation Funtion over the range of observable separations even though the cosmology and/or galaxy formation model are different. Moreover, with similar galaxy formation models, different cosmologies can result in both the same TPCF and the same PVD.

进而发现:(1)即使宇宙学和星系形成模型均不同,模型Lyman Break星系在可观测的尺度上也可以表现出一样的两点相关函数;(2)另外,不同的宇宙学模型可以预言相同的TPCF和PVD;(3)然而,不同的星系形成模型即使保持TPCF一样,也将预言截然不同的PVD。

Now if we compare the prodigious diversity of upbringings and of ways of life which prevail among the different classes in the civil state with the simplicity and uniformity of animal and savarage life, where everyone eats the same foods, live in the same style and does exactly the same things, it will be understood how much less the difference between man and man must be in the state of nature than it is in society, and how much natural inequality must be increased in the human species through the effects of instituted inequality.

&因为当一个巨人和一个侏儒在同一条道路上行走时,两个人每走一部,巨人的优势就会增加一些。现在,如果我们把文明社会中流行于各阶层的不同教育和生活方式惊人的多样性与吃、住、行个体间毫无差别的动物和野蛮人生活的简朴一致进行比较,就会理解自然状态中人与人的差别为什么会比文明社会中人与人的差别少得多,并且也会理解为什么自然的不平等被人为建立的不平等加深了许多。&

Those who imagine that talk about God in human language must affirm and deny the same thing at the same time and in the same respect have a different view of the relation between the divine mind and the godly person's mind than did Paul.

那些想象谈论上帝在人类的语言是应该予以肯定和否定同样的事情在同一时间内,并在同样的尊重,有不同意见的关系,牢记神圣和神圣的人的心灵比保罗。

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相关中文对照歌词
I'm Not The Same
You Will Always Be The Same
The Same Hello, The Same Goodbye
The Rover / Achilles Last Stand / The Song Remains The Same
John The Conqueror (Truth Is Always The Same)
The Song Remains The Same
The Sun Don't Shine On The Same Folks All The Time
Children Of The 90s (I'm Not The Same)
Nothing Looks The Same In The Light
The Same (Doesn't Feel The Same)
推荐网络例句

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特,那个是爱朗。

Articulate the aims, objectives and key aspects of a strategic business plan.

明确的宗旨,目标和重点战略业务计划。