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Results Same conservative sites and key catalytic sites existed among SjLDH and LDHs from other species. Similarity of SjLDH compared to CsLDH, TvLDH and HsLDH was 75%, 17%, 58%-60% respectively. Phylogenetic analysis demonstrated that the evolution relation between SjLDH and DmLDH was closer than the relation between SjLDH and CeLDH, the relationship between SjLDH and HsLDH-B,-C was closer than HsLDH-A. Three trans-membrane regions were found, the region of 98aa-106aa in three hydrophilia regions located outside of membrane was inferred as the major antigen epitope. This antigen epitope had significant difference with LDHs from protozoon （ Pf ., Tg ., Tv .） and had 1-3 amino acid residue difference with MmLDH, HsLDH-A,-B,-C, and was the same with CsLDH. One of the key catalytic residues and substrate binding loop were located in this region. Tertiary structure demonstrated that 98aa-106aa was on the surface of the protein and formed a substrate binding loop, other two key catalytic sites were at the position near the loop.

结果 SjLDH与其他物种的同源序列含相同的保守位点及催化活性位点，与华支睾吸虫LDH同源性最高为75％，与阴道毛滴虫LDH同源性最低为17%，与人LDH（HsLDH-A，-B，-C）的同源性为58%~60％； SjLDH与果蝇的进化距离较秀丽隐杆线虫为近，3种人LDH中与HsLDH-B、-C的进化距离较HsLDH-A为近；该蛋白具有3个跨膜区域，3个高亲水性区域，主要的线性表位98aa~106aa位于膜外，与原虫LDH相同区域差异显著，而与其他LDH有1~3个氨基酸的差异，关键催化位点之一及底物丙酮酸结合区域位于该区域，蛋白质同源模建分析表明该区域位于蛋白表面形成环状结构，3个关键催化位点位于该区域或在其附近。

Catherine and Xi Sike cliff of love is based on their personality and interests entirely satisfied that the same basis, the love between them is mainly the same spirit rather than the appearance of the mutual attraction, is the soul rather than the desire to fit the needs of the , on the last time they met in that storm-like unquenchable passion, nor have the slightest passions mixed composition, which also in the hands of the writers of the ideal, pure love for himself.

凯瑟琳与希思克利夫的爱情是以他们的性格和兴趣完全认为同为基础的，他们之间的爱情主要是精神上一致而非外貌上的相互吸引，是心灵的契合而非欲望上的需要，就在他们最后一次见面中那狂风暴雨般不可遏制的激情，也不夹杂有丝毫的情欲成份，这也寄托了作家对理想的、纯洁的爱情的向往。

Secondly, method of Taylor series is used to approximate the slow manifold and the O approximation of the slow manifold is given; slow dynamics such as saddle node bifurcation which has been proven to be the same as that for the full system is analyzed; at the same time, fast dynamics including bifurcation conditions and domain of attractor of the stable equilibrium are also analyzed; the attracting region which consists of the stable manifold of the unstable equilibrium and stable equilibrium is given; we found that when the inner flux decay increases, the domain of attractor is enlarged.

首先，给出了电力系统SMIB模型的时间尺度分解，得到标准的奇异摄动模型；其次，利用幂级数方法，给出了慢流形的O近似M〓，分析了在慢流形M〓上的慢动态，得到了慢子系统的鞍结分支值，并证明此分支值与原系统的鞍结分支值相同；分析了快子系统的平衡点和全局分支的存在条件，给出由不稳定平衡点的稳定流形与稳定平衡点形成的吸引域；并研究内部电压对稳定域的影响，发现稳定域随内部电压增加而扩大；最后给出整个系统的稳定平衡点的近似吸引域。

On the flexibility of tactics after more than a pass is flexible and I am not here to say, I will talk about the main points: 1, if a D does not increase blood bears do not return to open the same violent avoid opening kill, and disappear, lost to blind DZ, equipment can only say that a bit far worse; 2, if the Bears D to open violent response, you should not continue to attack and should attack you = reply to the HP, do not control, even if not control him, and he will not stay in the number of anger, not much damage output; Bears D on a human five seconds halo skills CD for 30 seconds, if the DZ had tried to avoid being hit, the general gambling Bear D will not take the skills, if HP Xiong D is less than 50% when using this skill, take one's time to lift the recommendations to see if he is a change or continue to bear form, the early lifting of the Bears D changed their tactics, then, and fight back, you will be injured, if he changes were increases in blood or take the skills, you will be the speed of response, and who is in the form of the XD is too fragile, and this time a counter-attack by DZ, the basic die is disabled. 3, proposed by the drug for the treatment and slow down (do not underestimate the slowdown, though XD turned the speed of the lifting of restrictions on DEBUFF, but turned when it is more dangerous, and at the same time suggest that a variety of turned to see Wang XD) Of course, bring their own drugs slow down the damage can be free with the.

之后的战术就灵活多了，打发是灵活的，这里我就不继续说，我就说说要点：1、如果一个熊D不加血不用开狂暴回复一样杀掉开闪避、用消失、丢致盲的DZ，只能说装备上差的有点远了；2、如果熊D开狂暴回复，你就最好不要继续攻击了，应为你的攻击=回复其HP，也不要控制了，就算不控制他，他怒气也不会留多少，伤害也输出不了多少；熊D就一个晕人5秒的技能CD为30秒，如果DZ开了闪避，一般熊D不会赌博的放这个技能，如果熊D是HP少于50%的时候用这个技能，建议不忙解除，看看他是变人还是继续熊形态，早早的解除的话熊D改变战术，和你拼命，你会很受伤，如果他变人加血或者放技能了，你就要速度反应了，人形态的XD是在是太脆弱了，这个时候被DZ一个反击的话，基本不死也就残废了。3、建议上毒为减治疗和减速（别小看减速，虽然XD变身解除限制速度的DEBUFF，但是变身时候是比较危险的，同时建议各位XD看看各种变身宏）当然了，毁伤自带减速毒就可以自由搭配了。

In fact, on one hand, it is often found that two graphs with the same number of verticesand the same connectivity(edge-connectivity) may result in entirely different forms after a minimum disconnecting set of vertices is removed, one may be totally disconnected while the other may consist of a few very stable components, and thus be much easier to reconstruct.On the other hand, we often face this question: how easily can we disrupt a system at the least cost, on the contrary, when we set up a system, especially a communication system, we try our best to make it as stable as possible that it can be reconstructed easily if it do get disrupted .

对于两个具有相同点数，具有相同点连通度的图，从图中分别去掉使图不连通的点集后，所得图的结构可能完全不同，这是因为点连通度、边连通度和局部点、边连通度等连通性参数仅反映了系统被破坏的难易程度，而对系统遭受破坏的程度并没有明确的反映。

The results showed that:The reductions in the shoot and root dry weights, leaf area,stern diameter of grafted plants were lower than those of ungrafted plants under the same NaCl stress.Na~+,MDA,H_2O_2 contents,O_2~- producing rate,Na~+/K~+ ratio, Na~+/Ca~(2+) ratio were lower,whereas K~+,solube sugar,shoot water content,SOD,POD, CAT,APX activity,the stability of AsA/DHA,Photosynthetic rate,transpiration rate and stomatal conductance in the leaf of grafted plants were higher than those of ungrafted plants at the same NaCl stress.Intercellular CO_2 concentration of grafted plants decreased whereas that of ungrafted plants increased significantly under salt stress.

结果表明，盐胁迫下，嫁接苗地上部和根干重、叶面积、茎粗受抑制的程度显著低于自根苗；嫁接苗叶片中Na~+、MDA、H_2O_2含量、超氧阴离子产生速率、Na~+/K~+和Na~+/Ca~(2+)比值显著低于自根苗，而叶片中K~+含量、可溶性糖含量、地上部含水量、SOD、POD、CAT、APX活性、AsA/DHA的稳定性、光合速率、蒸腾速率、气孔导度显著高于自根苗；嫁接苗的胞间CO_2浓度显著下降，自根苗的则显著上升。

A Whoever, without the consent of the patentee, marks upon, or affixes to, or uses in advertising in connection with anything made, used, offered for sale, or sold by same person within the United States, or imported by the person into the United States, the name or any imitation of the name of the patentee, the patent number, or the words "patent,""patentee," or the like, with the intent of counterfeiting or imitating the mark of the patentee, or of deceiving the public and inducing them to believe that the thing was made, offered for sale, sold, or imported into the United States by or with the consent of the patentee; or Whoever marks upon, or affixes to, or uses in advertising in connection with any unpatented article the word "patent" or any word or number importing the same is patented, for the purpose of deceiving the public; or Whoever marks upon, or affixes to, or uses in advertising in connection with any article the words "patent applied for,""patent pending," or any word importing that an application for patent has been made, when no application for patent has been made, or if made, is not pending, for the purpose of deceiving the public - Shall be fined not more than $500 for every such offense.

a未经专利权人之同意，意图伪造或仿造专利权人之标示，或意图欺瞒公众，使公众误认其产品为专利权人于美国境内所制造、要约销售或销售，或由专利权人输入至美国境内，或经其同意者，而将专利权人之姓名或仿名、专利号码，或"专利"、"专利权人"等类似之字样、标示、粘贴或使用于其所制造、使用、要约销售、销售于美国境内或输入美国境内之任何产品；或以欺瞒公众为目的，将"专利"或任何隐含已获准专利之字样或号码，标示、粘贴或使用于未准专利产品之广告上；或以欺瞒公众为目的，于申请专利前，或虽申请而已遭核驳之状态下，将"专利申请中"，或"专利审查中"，或隐含专利已提出申请等字样，标示、粘贴或使用于其产品之广告上者－每一违反行为，处五百元以下之罚金。

Perhaps i say like this,it's not honesty.my heart just the same feel a little lose.when all is said and done,it's a decision which is forced to make up.it's not my voluntary.it always ask us to think that we are the people to be cast away.at the same time,because of my parents,i also hope to accompany with them when they are still healthy.so i also lean to come back to china.on the other hand,i can understand the decision of the school.after all the school broke ground only a short time,it has many necessary consumption,it needs many money to supply.but we are too valuableness for the school,even if we are not satisfied with the treatment and the salary.only for the situation that school has not gived us any explanations,and then has made the decision.i think it is not done.kick down the ladder,it's not a good feeling.well,china has a old saying,"gather well,and fall apart well."

或许这样说，并不是很诚实。内心还是有些失落的，毕竟这是被迫做出的决定，还不是我主动的，总有一些被人抛弃的感觉。但是，也因为我一直考虑到父母，我希望在他们身体健康的时候，自己可以陪伴在他们身边，所以还是很倾向回国的。另一方面，我也可以理解学校的决定。毕竟学校创办之初，有许多需要花钱的地方，我们对于学校略显"昂贵"了一些，即使我们本身并不满意。只是学校在没有给我们任何说法的情况下便作出了这样的决定，还是有些失妥当的。有些过河拆桥的味道。罢了，中国有句老话，"好聚好散"。

After treatment of benzamide which is an inhibitor of poly polymerase,β-galactosidase activity assays and PCR analysis were performed to test the deletion of LacZ gene and c-Ha-T24ras gene. The results showed that:(1)LacZ gene was deleted from both A13.4-NIH3T3 cells and B12.7-NIH3T3 cells, but neigher from A13.4-HeLa cells nor B12.7-HeLa cells. So it is possible that there is cell line specificity in gene deletion induced by BA.(2) In A13.4-NIH3T3 cells, LacZ gene and c-Ha-T24ras gene were completely deleted at the same time.(3) The transcription activity of exgenous DNA fragment may have same effect on its sensitivity to the deletion induced by BA.

用聚ADP核糖聚合酶的NAD位点抑制剂苯甲酰胺分别处理四种转化细胞后，检测细胞中整合的外源LacZ基因与c-Ha-T24ras基因的删除情况，结果如下：BA诱导的基因删除可发生于NIH3T3转化细胞系，但不能发生于HeLa转化细胞系；位于同一外源表达载体上的LacZ基因与c-Ha-T24ras基因的删除过程是同步的；外源整合DNA片段的转录强度可能直接影响其被BA诱导删除的敏感性。

You should let your mom know of that. Because your mom is the one who loves you the deepest, and at the same time, he has your deep love. The distances between your mom and you, you and him, one is so short and the other is that far. And you are the crosspoint who make the two unrelated life be involved in this same space.

请你一定要让妈妈知道，因为妈妈是最爱你的人，而你也深爱着他，一个很近一个很远，而你是将两个无关爱的生命体柔和于同一空间出现的凝结点。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。