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Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.
发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。
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The results showed that food ingestion and frequency of the polecat in constant food patches were higher than that in the variable food patches and time of the polecat was the same between two patches during the training period. Only the frequency of the polecat in constant food patches was higher than that in variable food patches when the polecat was starved for 1 day. Its foraging pattern between the two patches was the same as that during the training period when the polecat was starved for 2 days. Meanwhile, the food ingest ion amount in the two patches was not affected by starvation, but the time and frequency of the polecat using the two patches significantly decreased when starvation increased.
研究结果表明,训练期实验中,艾虎对固定食物量斑块中的取食量和利用频次明显高于变化食物量斑块,而对两个斑块的利用时间基本相同;艾虎饥饿一天后对固定食物量斑块中的利用频次明显高于变化食物叠斑块,而对两个斑块的利用时间和取食量基本相同;艾虎饥饿两天后对两个斑块利用程度与训练期的结果相同:同时,艾虎在两个斑块中的取食量均与饥饿程度无关,而对两个斑块的利用时间和利用频次均随饥饿程度明显降低。
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"While preious research has demonstrated that arious types of stem cells can turn into cells that express the proteins consistent with smooth muscle, this is the first study that shows that the cells we generated hae the same functional properties as smooth muscle, as well as express the same proteins," said Jeffrey Ross, Ph.D., research associate at the Stem Cell Institute.
"以前的研究已经证实多种不同类型的干细胞可以被转化成表达与平滑肌细胞相同蛋白的细胞,这个实验首次显示,我们得到的细胞由于平滑肌相同的特性,表达一样的蛋白,"干细胞研究所的研究员Jeffrey Ross博士说。
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Economic globalization extended the capacity of world market, the development that is each country economy brings larger mobile space, offer more development opportunity, the characteristic that also makes world market risk increases revolution of; new science and technology to deciding structure of prospective world industry at the same time and the way that economy grows, for economy development provides new power and new field, also make the development of traditional industry faces greater pressure, competitive advantage tilts to the state that has capacity of advanced technique innovation further the structural adjustment of limits of; whole world will make area of world economy growth widens further, the shirt-sleeve rate of each country economy rises further, at the same time each country also do one's best holds vantage ground in new international division of labor.
经济全球化拓展了世界市场的容量,为各国经济的发展带来更大的活动空间,提供更多的发展机遇,同时也使世界市场风险加大;新科技革命决定着未来世界产业结构的特点和经济增长的方式,为经济发展提供新的动力和新的领域,也使传统产业的发展面临更大的压力,竞争的优势进一步向拥有先进技术创新能力的国家倾斜;全球范围的结构调整将使世界经济增长面进一步拓宽,各国经济的融合程度进一步提高,同时各国也力求在新的国际分工中占据有利地位。
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Consider to make clear in recent years, second acid is calcic - Bowman does not move bacillus clinical bacterium of single born of the same parents of the detached rate that go up and verdigris holiday comparatives, have the tendency that year after year rises, the fundamental condition pathogenic bacteria that already became the infection inside the courtyard [1] , have to a variety of antibacterial content be able to bear or endure higher property of a medicine [1, 2] , for this, we are monitored and statistic analysed my courtyard to came 1998 2004 7 years a second acid is calcic - the clinical infection condition that Bowman does not change bacillus, to 14 kinds of antibacterial content be able to bear or endure property of a medicine and be able to bear or endure medical tendency, sum up a report to be as follows now. 1 material and method all data all come from 1.1 basic materials to came on January 1, 1998 my courtyard outpatient service was mixed on December 31, 2004 the specimen sending check of inpatient, via clinical microbiology the lab is fostered and classics appraisal is second acerbity calcium - Bowman does not move bacillus to affect case of illness, with clinical and commonly used antibacterial at the same time content makes the kill that medical quick detects. 1.2 bacteria appraisal is mixed medical quick experiment uses live thing VITEK32 of Mei Li dust is full automatic the bacterial appraisal of bacterial analysis system and its form a complete set gets stuck and medical quick card undertakes detecting, 1998~2000 year use GNI appraisal gets stuck, 2001~2004 uses GNI+ card.
近年探究表明,乙酸钙-鲍曼不动杆菌临床上的分离率和铜绿假单胞菌相当,并有逐年上升的趋向,已成为院内感染的重要条件致病菌[1],对多种抗菌药物有较高耐药性[1,2],为此,我们监测并统计分析了我院1998年至2004年七年间乙酸钙-鲍曼不动杆菌的临床感染情况,对14种抗菌药物的耐药性及耐药趋向,现总结报道如下。1材料和方法1.1基本资料所有资料均来自于1998年1月1日至2004年12月31日我院门诊和住院病人的送检标本,经临床微生物学实验室培养并经鉴定为乙酸钙-鲍曼不动杆菌感染病例,同时以临床常用抗菌药物作药敏检测的结果。1.2细菌鉴定和药敏试验采用生物梅里埃VITEK32全自动细菌分析系统及其配套的细菌鉴定卡和药敏卡进行检测,1998~2000年使用GNI鉴定卡,2001~2004使用GNI+卡。
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Sequence analysis and phylogenetical study showed that this cDNA contained 778 nucleotides, with a 627 bp open reading frame flanked with a 44 bp 5' UTR and a 107 bp 3' UTR. The encoded 208 amino acids housefly Attacin shared a high similarity of 50%~70% with that of the other dipterous insects. In addition, the phylogenetical analysis also indicated that the Attacin from housefly was in the same branch with those of other species, suggesting that they come from the same ancestor.
家蝇Attacin的cDNA全长778 bp,其中包括627 bp的开放式读码框,44 bp的5ˊ末端非编码区(5ˊUTR)和107 bp的3ˊ末端非编码区(3ˊUTR),相应的蛋白产物含208个氨基酸;与其他双翅目昆虫的Attacin具有很高的相似性,约50%~70%;以邻接法(Neighbor-Joining,NJ)所构建的系统关系表明家蝇的Attacin与其他双翅目昆虫的Attacin起源于共同的祖先。
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The comparative studies of lithogeochemistry for the volcano-intrusive complexes both from Jiulongzhang and from xiangshan reveal that the chemical compositions of the volcano intrusive complexes in the two areas are very close, and the trace element spidergrams and REE patterns basically coincide with each other. It is also shown that the two volcano-intrusive complexes have the same source features and the active continental margin tectonic settings. At the same time, it is found that, from early volcanic magma activities to later intrusive magma activities, the content of SiO2 and the Rb/Sr ratio decreased, on the contrary, the content of MgO and the ratios of K/Rb, Ba/Rb and LREE/HREE increased. It is shown that the hypabyssal or ultra-hypabyssal intrusives are not the products of the volcanic magmatic differentiation.
两地区的火山-侵入杂岩的岩石化学成分及其变化特点极为相近,微量元素蛛网图和稀土配分型式也基本一致,它们具有相似的岩浆过程和统一的活动陆缘构造背景;同时,从早期火山岩浆活动到晚期浅成-超浅成岩浆侵入活动,其w(SiO2)和w/w的值降低,而w以及w/w、w/w和w/w的值增高,晚期浅成-超浅成侵入岩与早期火山岩没有直接的分异演化关系。
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The test results showed that: Young Modulus of undisturbed Kitan clay is much bigger than that of reconstituted one under the same stress level; Young Modulus of the same clay under high strain rate is bigger than that of small strain rate; Undisturbed Kitan clay showed perfect linear elastic property within small strain smaller than 0.01
试验结果显示:在相同应力水平条件下,未扰动Kitan粘土的杨氏模量大于重塑土;对于同一种粘土,在应变率大的情况下,它的杨氏模量同样大;在应变小于0.01%时,未扰动Kitan粘土呈线弹性。
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The new opinion and mechanism that the simultaneously adsorption of complex pollution of both organic and heavy metals will be achieved on soil modified with amphoteric surface modifying agent, which have both charged group and organophilic group on one ASMA molecule, were proposed at first time, and its rationality was testified at the same time; The 50% was the critical CTMAB modifying ratio of soil CEC at which the organophilic bond modifying mechanism was emerged; The thermodynamic characteristics of adsorption of OPT and HMP on OMS were discovered ; The study revealed the surface peculiarity of OMS that the modifying agent were distributed at uneven scatter mode and multi-characters surface were coexisted on surface of OMS, revealed the adsorption peculiarity of OMS that multi-mechanisms were coexisted, confirmed the unequal charge amount modifying mode of OMS in which dual-modifying mechanism of both ion exchange and OPB were processed at the same time, and confirmed that the adsorption hobby to CTMAB was higher than that to Ca2+ on Lou soil.
首次提出了用两性表面修饰剂对土壤进行修饰改性,利用两性表面修饰剂所具有的荷电基团和疏水基团达到对土壤中有机、重金属污染物同时吸附的思路和机理,并通过实验证实了所提设想的合理性;发现了土壤CEC值的50%修饰比例是CTMAB开始显现以疏水键机制对土土样表面进行修饰的临界点;揭示了有机修饰改性土对有机物、重金属吸附过程的热力学特征,填补了该项研究工作的空白;阐明了修饰剂在改性土表面呈分散态的非均匀分布,改性土表面具有不同性质表面共存的表面特征和对污染物多机制共存的吸附特征,确定了阳离子表面修饰剂在土表面的修饰是一种离子交换和疏水键结合两种机制相伴并行且为非等电荷量交换的修饰反应模式,以及土具有对CTMAB的吸附偏好性远大于对钙离子吸附偏好性的特征。
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Dusk Looking into the sunset I can't help but notice that despite her beauty, a sense of struggle and hopeless surround the sky . Deep inside you realize that this day is gone, and everything that It had brought is lost forever. Every thought,every action,every dream,every hope, every sight,every sound is gone. There is no chance of every being returned the same, exactly the same. For every moment has a limit to what it can capture, Every memory has a limit to what it had retrieve. And the colours in the sky try to entertain us. one last act with painted smiles, for they too know that nothing can be done to save the day. So futile their attempt to comfort our fear of the night. our horror as we try to find our way, like children who wander into a forest and never return. I am ingratiated by the sunset because of her sensitivity as she tries to push the darkness back for just a moment more. But like so many times before....to no avail!
黄昏 凝望夕阳,我无法抑止地看到,无论她是如何的美丽动人,挣扎和绝望依然萦绕天幕,内心深处,你明白今日已逝,它所带来的一切也永远消失了,每一脉思想,每一次行动,每一个梦想,每一线希望每一幅景象,每一缕声音,都消逝而去,一切都不可能还复如初,和原来一模一样,只因每一个时刻所能捕捉的东西是有限的,甚至每一段记忆所能缅怀的一切也是有限的而天空中的绮丽色彩努力让我们快乐起来,最后上演的是五彩缤纷的微笑,因为它们也知晓,做什么都无法留住时日,它们想安慰我们对夜的恐惧,而又如此徒劳无益,虽然恐惧,我们依然寻找出路,宛若在林中的孩心,彷徨不知归路,我满心欢喜,只因夕阳的敏感,她竭力将黑暗推回,仅仅为了再多驻留一刻,可却犹如以往,一切都无济于事。。。。
- 相关中文对照歌词
- The Same Love That Made Me Laugh
- The Same Love That Made Me Laugh
- This Ain't the Same Town (That I Painted Red)
- The Same Love That Made Me Laugh
- The Same Love That Made Me Laugh
- 推荐网络例句
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The split between the two groups can hardly be papered over.
这两个团体间的分歧难以掩饰。
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This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.
这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。
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The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.
聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力