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ANP and CX43 began to express at 2nd week after induction and increased gradually,about 60% of the resulting myogenic cells were positive at 4th week after induction ,they were negative for uninduced cells.hMSCs'surface antigen profiles obtained by Flow Cytometry were negative for CD31\CD34\CD45 before and after induction,but CD90 expressed higher after induction while was weak positive before induction(P.05). Apotosis index was correlated with the cultural time after induction,The apoptosis rate of induced hMSCs was remarkably higher than control group(P.01),and the variation between groups was notable(P.05),the cell cycle analysis showed that the percentages of G_0/G_1phases were reduced significantly after induction. The expresstion levels ofβ-MHC and CTNT mRNA were undetectable before induction,began to increase at 1st、4th week after induction,reached the peak at 6th week and decreased after that,the expression of Bcl-2 and Bax mRNA varied regularly after treated with 5-azacytidine. hMSCs'resting membrane potential、range and rate of depolarization were heightened gradually after being induced.
结果:hMSCs诱导前为纺锤形,诱导后第2天部分细胞即开始发生形变,呈球形或短棒状,1周后胞浆中颗粒增多,约20~30%细胞边缘呈毛刷样变化;hMSCs表面抗原CD31、CD34、CD45在诱导前后差异无统计学意义,CD90未诱导时表达呈弱阳性,诱导后明显增高(P.05);ANP和CX43在诱导前无表达,诱导后第2周开始表达且表达随时间逐渐增强,但CX43在诱导后第5周表达量开始降低。hMSCs诱导后凋亡指数随诱导后培养时间增加,低浓度诱导组低于标准浓度诱导组,组间差异有统计学意义(P.05),G_0/G_1期细胞比例诱导后较对照组显著减少(P.05);β-MHC和CTNT基因分别在诱导后第1周和第4周时表达开始增强,在第6周时均达到高峰,第8周时表达开始衰减,Bcl-2、Bax基因表达呈时间依赖性变化,hMSCs经诱导后随心肌样细胞特征的表达膜静息电位、去极化幅值和去极化速率逐渐增高。
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Our results shown: Compared with those of the unradiated rats, the number of S-100 protein immunoreactive positive glial cells in the brains of the irradiated rats increased gradually. More S-100-positive glial cells with multiple long processes and hypertrophic cell bodies were also observed in these areas.
在此基础上,又做了同一照射剂量(20Gy)照射大鼠半脑后不同存活期间(id,7d;14d,Zld,28d)大鼠全脑S-100动态变化的观察,发现其规律如下:在上述时间点大鼠脑内各部位卜刁m蛋白免疫反应细胞数量进行性增加,伴有 S-100免疫阳性细胞的胞体逐渐增大和交起增多,与正常对照组具有明显的差异。
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Results There were no sinificant difference in incidence of aneuploid and highs synithesis phase fraction, the size and distribution of AgNOR between unrecurrent and recurrent tumors, and there were sinificant difference between benign and malignant tumors, and there were singnificant differenct in the number and shape of AgNOR between the groups, and a linear positive correlation was observed between that obvious positive correlation was observed between highs SPF and number of AgNOR.
结果 异倍体率,高S期细胞比率率,AgNOR大小和分布在无复发与复发组间无显著差异,而良性与恶性肿瘤间有显著差异;AgNOR数目和形态各组间有显著差异;高SPF率与AgNOR均能反映其细胞增殖活性。
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Genomic DNA of positive transformed plants; 6, 7: Genomic DNA of negative transformed plants; 9: genomic DNA of untransformed plants; 10: Positive control of 3G1MHpCAMBIA2301; 11: NTC; 12: DL 2000 marker.
2.2 转基因拟南芥的筛选选择培育后获得转基因拟南芥植株。从其叶片中提取微量DNA, PCR鉴定,可扩增出750 bp左右片段(图3),表明3G1MHpCAMBIA2301基因已整合到拟南芥染色体中。
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Meanwhile, positive human sera of HCV challenged vaccina ted monkeys after 6 weeks immunized, ALT value rose transiently in monkeys vacci nated by PCX. It was positive in monitoring RNA of HCV using RT-PCR in monkey s era.
同时,在免疫后6周用人HCV阳性血清攻击猴子,免疫PCX的猴子出现一过性ALT升高,在攻击后三周内用 RT-PCR检测到猴血清内HCV的RNA阳性。
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Meanwhile, positive human sera of HCV challenged vaccina ted monkeys after 6 weeks immunized, ALT value rose transiently in monkeys vacci nated by PCX. It was positive in monitoring RNA of HCV using RT-PCR in monkey s era. The result demonstrated that Rhesus monkey immunized by multi-epitope PCX could elicit high-level immune responses.
近年来,由于多表位抗原概念的兴起,在针对EB,HIV等病毒感染的疫苗研究中引入了多表位抗原疫苗[门,这种思路使对HCV疫苗的研究有了新的切入点,F.r.i[']等用 HCV.H77株 E蛋白的多个表位所制备的兔血清与HCV
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Results:Sixteen were HP positive by rapid urease test,twenty were HP positive by histology,among 35 cases of varioliform gastritis.
痘疹性胃炎不是淋巴细胞性胃炎的表现,它可能与HP感染或机体对某些物质过敏有关
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Results 336strains of pathogenic bacteria were separated from the urine sample of2865cases and the detectable rate was11.73%of the336separated strains the number of strains of gram negative bacilli was226,the gram positive cocci is92and the fungi is18.the positive rate of escherichia coli was the highest followed by co-agulase negative staphylococci,klebsiella pneumoniae and blue verditer pseudomonas.highest resistance to penicillins and cephalosporins was obsered.
结果 从2865份尿标本中分离出336株病原菌,检出率为11.73%。其中革兰阴性杆菌226株,革兰阳性球菌92株,真菌18株。阳性率最高的是大肠埃希菌,其次是凝固酶阴性葡萄球菌、克雷伯菌、铜绿假单胞菌。药敏结果对青霉素类、头孢类抗生素的耐药率较高。
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Women who were diagnosed positive for having any VIA, VILI and colposcopy inspection or for those women who were diagnosed negative for VIA, VILI and colposcopy but with positive result of HPV or TCT test underwent cervical biopsy directly and endocervical curettagewhen necessary.
对VIA、VILI及电子阴道镜三项检查中任何一项存在子宫颈可疑病变的妇女行子宫颈活检;对以上三项检查均未发现可疑病变,但子宫颈脱落细胞HPV阳性或TCT结果阳性的妇女,2周后给予子宫颈活检。
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Results]CEA in 51 patients were positive(69%),9 cases were positive on cellular diagnosis in sputum,56 patients were diagnosed lung cancer by CT, Percutaneous needle biopsy were performed on ,42 patients were treated by operation, and 46 cases(62%) were virtued as lung cancer.
结果]74例患者CEA增高51例(69%),痰脱落细胞学发现癌细胞9例(12%),CT诊断肺癌56例。26例行经皮穿刺肺活检术,42例手术治疗,术后诊断肺癌46例(62%)。
- 相关中文对照歌词
- L'unité
- Positive
- Katie's Tea
- La Colline Des Roses
- Stay Positive
- Start It Up Y'all
- La Dance Ghetto
- The Power Of Positive Drinking
- Positive Vibes
- Three Minute Positive Not Too Country Up-Tempo Love Song
- 推荐网络例句
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Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.
目前大多数的研究表现为在变形后的受控Petri网基础上,利用各种方法求得各种逻辑型、结构型控制器,解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。
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On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.
一方面,有讨论,工程理事会和联盟关于延长工作时间短至9月底。
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