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Sarcomeric actin expression was positive on day 10 after differentiation. the positive expression was in plasma. on day 6, plasma and organelle in cells increased, tight junction and intermediate junction were found and there was apophysis in cell surface and microfilament in apophysis.

电镜观察,未分化细胞为圆形或椭圆形,细胞间连接松散,细胞质较少,细胞器不发达;诱导后6 d,细胞间出现紧密连接和中间连接,细胞质增多,部分细胞表面出现突起,突起内可见微丝。

Such results are in agreement with those of the study by Upadya et al., who showed a link between positive cumulative fluid balance and weaning failure.

预测脱机失败的最佳BNP域值是275 pg/ml,此结果与Upadya等的研究一致,Upadya等发现了液体正平衡(positive cumulative fluid balance)和脱机失败存在关系。

Egr-1 mRNA and Egr-1 protein hadn't been found in the normal vein. The expressions of Egr-1 mRNA and Egr-1 protein had biphasic changes. By reverse transcription-PCR and in situ hybridization, we found that the level of Egr-1 mRNA rose at 1 hour after graft, the expression of Egr-1 mRNA was (35±7)%. Decline at hour 6, 24 and day 3, the positive rates of Egr-1 mRNA were (8±2)%,(8±6)% and (8±4)% respectively. Reincrease at day 7, a peak at day 28, the positive rate of Egr-1 mRNA was (45±6)%(compared with other phase, P<0.01). At day 42, the expression of Egr-1 mRNA declined again. Immunohistochemical staining and Western blot revealed Egr-1 protein had expressed at hour 2 early phase, the expression of Egr-1 protein was (30±5)%, and until to hour 6. The level of Egr-1 protein was decrease at hour 24 and day 3, the positive rates were (7±3)% and (7±8)% respectively.

结果 自体静脉移植后,Egr-1 mRNA和Egr-1蛋白的表达呈双相变化,即移植后1 h, Egr-1 mRNA表达迅速升高,阳性率为(35±7)%,6 h、24 h及3 d时下降到较低水平,阳性率分别为(8±2)%、(8±6)%和(8±4)%,7 d时又再升高,28 d时达高峰,阳性率为(45±6)%,此与其余各时点比较差异均有统计学意义(P<0.01),42 d时,Egr-1 mRNA的表达再次下降;移植早期(2 h)即有Egr-1蛋白的表达,阳性率为(30±5)%,并持续至6 h,24 h~3 d表达下降到较低水平,阳性率分别为(7±3)%和(7±8)%,7 d时又再升高,至移植后28 d,Egr-1蛋白的表达阳性率达到高峰,为(40±9)%,此与其余各时点比较差异有统计学意义(P<0.01)。

Results ①p53 positive expression rate in cervical carcinomas 85.71%( 30 /35) was significantly higher than that in normal cervixes 6.67%( 2 /30),P= 0.001.②p53 expression is correlated with the differentiation state and pathologic grades.The positive expression rate of p53 in the cervical carcinomas of grades Ⅰ 57.14%(8 /14) was significantly lower than that in the cervical carcinomas of grades Ⅱ 86.67%(13 /15) and grades Ⅲ 100.0%(6 /6),P<0.05.③The positive rate of PCNA in the cervical carcinoma of grade Ⅰ 64.29% was signifi cantly lower than that in the grade Ⅱ 93.33% and grade Ⅲ 100.0%,P<0.05.Conclusion Our data indicate that the alteration of p53 is involved in cervical carcinoma.

结果 ①P53蛋白在宫颈癌组织中的表达率为85.71%(30 /35),明显高于在正常宫颈组织中的表达率6.67%( 2 /30),P = 0.001;②P53蛋白阳性表达率与宫颈癌组织的病理学分级有关,P53在宫颈癌Ⅰ级中的阳性表达率57.14%(8 /14)明显低于Ⅱ级86.67%(13/15 和Ⅲ级100.0%(6/6),P<0.05;③宫颈癌组织PCNA阳性表达率Ⅰ级64.29%明显低于Ⅱ级93.33%和Ⅲ级100.0%,P<0.05。

Results: From the third to sixth month of gestation, some small NOS positive cells could be seen in the lamina propria mucosae. With the fetal age increased, the number of NOS positive cells increased too, and at the same time, the positive reaction of NOS increased gradually.

结果:第3~6个月龄时,胃粘膜固有层内皆可见胞体较小的NOS阳性细胞分布,随着胎龄的增加,NOS阳性细胞数目逐渐增多,染色逐步加深。

The histopathologic changes included lymphocyte and monocyte infiltration,hyperplasia of synovial cells and small vessels,interstitial fibrosis,hyaline degeneration and cartilaginous metaplasia.The immunohistochemical observations showed that the high expressions of IL-1 and IL-6 were significantly different between the pathologic plicae and the control groups(P<0.01).The positive expressions of IL-1 and IL-6 were the synovial cells and monocyt-lymph cells in the pathologic synovial plicae.The positive expressions of MMP-1 and TIMP-1 have significant difference between the experiment and control group(P<0.01,P<0.05).The expression of MMP-1 was positive in synovial lining cell,monocyte,fibroblast,endothelial cell in small vessel and chondrocyte.The TIMP-1 expression was detected in the synovial lining cells and a small quantity fibroblast.

结果 正常滑膜皱襞和病理性滑膜皱襞在滑膜细胞增生及小血管增生、间质纤维化及玻璃样变、软骨化生组织学改变方面,差异均有显著性(P<0.01);IL-1、IL-6在病理性滑膜皱襞内的增生滑膜细胞、单核及淋巴细胞和在正常滑膜皱襞内的表达差异均有显著性(P<0.01); MMP-1、TIMP-1在病理性滑膜皱襞和正常皱襞内的阳性表达,差异具有显著性(P<0.01,P<0.05),MMP-1在增生滑膜衬里层细胞、单核和纤维母细胞、血管内皮细胞和软骨化生的软骨细胞呈阳性表达;TIMP-1只在滑膜衬里层细胞和少量纤维母细胞有表达。

Results Immunostaining of cNOS and iNOS were negative in the rectums of normal rabbits, whereas the results in the of rabbits infected with S. japonicum were strong positive. The positive immunostaining of cNOS was observed in mucosal capillaries and submucosal vessels. Besides in capillaries and submucosal vessels the positive immunostaining of iNOS was seen in schistosoma ova, ova granuloma, fibroconnective tissue and suppuratie cells.

结果 正常兔直肠壁cNOS和iNOS呈阴性反应,血吸虫病兔直肠壁为强阳性,cNOS主要分布在粘膜毛细血管和粘膜下血管壁;iNOS不仅见于上述血管壁,而且见于虫卵、虫卵肉芽肿周围、纤维结缔组织中、炎性细胞之间。

Results Immunostaining of cNOS and iNOS were negative in the rectums of normal rabbits, whereas the results in the of rabbits infected with S. japonicum were strong positive. The positive immunostaining of cNOS was observed in mucosal capillaries and submucosal vessels. Besides in capillaries and submucosal vessels the positive immunostaining of iNOS was seen in schistosoma ova, ova granuloma, fibroconnective tissue and suppuratie cells.

结果 正常兔直肠壁cNOS和iNOS呈阴性反应,血吸虫病兔直肠壁为强阳性,cNOS主要分布在粘膜毛细血管和粘膜下血管壁;iNOS不仅见于上述血管壁,而且见于虫卵、虫卵肉芽肿周围、纤维结缔组织中、炎性前列腺细胞之间。

In rat esophagus, the positive varicosities and fibres could be observed in the circular muscle layer and the muscularis mucosae layer at 21st day before birth. With the development of rat digestive tract, neurokinin A-immunoreactivity positive nerves could be observed gradually in the epithelium, submucosa, longitudinal muscle layer, myenteric plexus and submucosal plexes, while mature nerve fibres could be seen at one month after birth. 2. In rat stomach, the positive reaction of NKA initially happened in the myenteric plexus at the 14th day of embryo, and then appeared on circular muscles, longitudinal muscles, submucosal, muscularis mucosae, lamina propria and epithelium. 30 days after birth, expression of NKA is same as seen in adaulthood. 3. In rat small intestinal, the NKA-IR could first be found in the myenteric plexus of the duodenum, jejunum and ileum at 14th , 15th and 17th day before birth respectively. Then the NKA-IR occurred in the longitudinal muscle layer, circular muscle layer, intestinal villus, intestinal gland, muscularis mucosae, submucosa, submucosal plexes, mucosa plexus and deep muscular plexus.

结果 1、在食管,于胚胎21天的粘膜肌层和环肌层内观察到神经激肽A免疫阳性膨体纤维,出生后,随幼鼠的生长发育,相继在上皮,粘膜下层,纵肌层、肌间丛、粘膜下丛有NKA-IR表达,30天时已和成年鼠相似;2、在胃,首先于胚胎14天的肌间丛出现NKA-IR的表达,随发育相继在环肌、纵肌,粘膜下层、粘膜肌、固有膜及上皮内出现NKA-IR的表达,30天时具备成年鼠的分布特征;3、在小肠,分别于胚胎14、15、17天的十二指肠、空肠和回肠的肌间丛处出现NKA-IR的表达,随发育相继出现在纵肌、环肌、绒毛、小肠腺周、粘膜肌、粘膜下层、粘膜下丛、粘膜丛、深肌丛。

Results (1)The positive rates or the scorings of MMP-1,MMP-9and MVD were significantly higher in human gallbladder neoplasm than that in human chronic cholecystitis(P.01),But the positive rates of MMP-2in human gallbladder neoplasm were significantly lower than that in human chronic gallbladder cholecystitis(P.01).(2)The closely positive correlations were found among the expressive scorings ofMMP-1,MMP-9,TIMP-2,MVD and the clinicopathological type,differentiated degree,with or without metastasic tumor.

结果 (1)31例胆囊癌组织中MMP-1,MMP-9的阳性表达率及其评分和MVD计数均值(以CD34阳性计数)分别为16例(51.6%)和20例(64.5%),明显高于10例慢性胆囊炎组织1例(10.0%)和2例(20.0%),差异有非常显著性(P.01);胆囊癌组织中TIMP-2的阳性表达13例41.9%)则低于慢性胆囊炎组织9例(90.0%),差异有非常显著性(P.01)。

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I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。

The lawyer's case blew up because he had no proof.

律师的辩护失败,因为他没有证据。