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Results Compared with that in the NP tissues, the number of α-SMA positive cells increased significantly in BPH tissue, and the number of vimentin positive cells increased moderately in the stroma and prominently surrounding the acinus and in the basal layer. In BPH tissue, the myosin and ERα staining signal was lost in the stromal cells surrounding the acinus, and the positive staining cells gathered into bunches in the stroma far away from the acinus, while the positive cells were sporadically distributed in the NP tissue. The PCNA positive cells increased moderately in the stroma and increased significantly in the basal layer.

结果 与NP相比在BPH中,α-SMA阳性染色细胞显著增加;波形蛋白在间质中阳性染色细胞有所增加,在腺泡基底层及临近腺泡外层间质中阳性染色细胞明显增加;在临近腺泡外的数层间质细胞中肌球蛋白和ERα由部分阳性变为完全阴性染色,而在远离腺泡的间质中其阳性染色细胞由散在斑块状分布变为簇状密集排列;PCNA在间质中阳性染色细胞有所增加,在基底细胞层中阳性染色细胞显著增加。

The levels of schistosome circulating cathodic antigen in serum and urine were detected parallelly by McAb-Dot-ELISA. In 81 acute cases, the positive rates in serum and urine were 96.30% and 66.67%(P<0.01) but the combined positive (positive in serum and/or in urine) rate reached 100%, while in 109 chronic cases, they were 77.06% and 10.09%(P<0.01) but the combined positive rate was not increased. In 100 healthy individuals from non-endemic areas, no positives were detected in serum and urine with false positive rate of 0%. The cross reaction rates in serum and urine of 51 cases with clonorchiasis were 9.43% and 0% but all were 0% in 48 cases with ancylostomiasis.

提要 应用McAb-Dot-ELISA平行检测血吸虫病人的血清和尿样(浓缩20倍)中日本血吸虫肠相关趋阴极循环抗原。81例急性血吸虫病患者的血清和尿样的阳性率分别为96.30%和66.67%,而血清和尿样组合检测的阳性检出率提高至100%。109例慢性血吸虫病患者的血清和尿样的阳性检出率分别为77.06%和10.09%,组合检测的阳性检出率没有提高。100份健康人的血清和尿样的假阳性率均为0%。54例华支睾吸虫病患者的血清和尿样的交叉反应率分别为9.43%和0%。48例钩虫病患者的血清和尿样的交叉反应率均为0%。

The four most common phenotypes are A,B,AB,and O,referring to the type of antigen is presentor absenton the surface of a client's red blood cells,Routine testing usually involves only the Rh antigen.if an Rh-negative clinet receives Rh-positive blood,he or she will develop Rh antibodies,and future Rh-positive transfusions may cause a transfusion reaction .in pregnancy,antibodies from an Rh-negative mother may hemolyze fetal(erythroblastosis fetalis,or hemolytic disease of the newborn).

四种最普遍的表现型是A、B、AB、阿,这类型的抗原或表面上的一个客户的红血球、常规测试通常涉及到只有Rhantigen.if准备一Rh-positive接受血液,跟他或她将发展Rh抗体和未来的Rh-positive输血可能引起输血反应,在怀孕期间的母亲可能会产生抗体hemolyze北京(erythroblastosis fetalis或胎儿的新生儿疾病溶血试验确定具体)。

The uncertainty given is the expanded combined uncertainty and represents an estimated standard deviation equal to the positive square root of the total variance of the uncertainty of components.

The不确定性是膨胀的联合的不确定性并且代表估计的标准偏差相等与组分不确定性的总变化的正面方根。

aeroallergen specific ige antibody in the serum of 625 patients with allergic rhinitis were positive. total ige in serum was positive in 413 cases, accounting for 66.1%. the positive rate of total ige was less than that of aeroallergen specific ige. the positive rate of aeroallergen specific ige in order were mite mixture (84.32%), cockroach (19.04%), cypress (15.20%), cat-dog epithelia (13.12%), tree mixture (7.20%), ragweed (5.12%), humulus lupulus (1.76%), mugwort (1.76%), mould mixture (1.44%). in the two groups, the positive rate of aeroallergen specific ige in order were similar.

625例变应性鼻炎患者中吸入性变应原特异性ige阳性率依次为尘螨84.32%、蟑螂19.04%、柏树15.20%、动物毛皮屑组合13.12%、树木花粉组合7.20%、矮豚草5.12%,葎草1.76%、蒿草1.76%、霉菌组合1.44%;总ige阳性者413例(66.1%)明显低于特异性ige阳性率;两组中最常见的变应原相似;未成年组屋尘螨的阳性率及敏感程度明显高于成年组(p 0.01)。

2B8a was weakly reactive to neutrophils (23.72%) and negative for T cells, NK, DC, RBC and Plt. The antibody reacted to all 3 marrow CD34+ cells with an average positive rate of 39.33% while it was negative for G-CSF-mobilized CD34+ peripheral blood stem/progenitor cells (PBSC, 1.25%). Cell line analysis showed that the antibody notably reacted to three out of 4 cell lines (Raji, SMS-SB, Nalm-6 and Nall-1) with the positive rates of 98.78%, 98.61%, 94.93% respectively and weakly to one of them with 5.68% in B lineage cell lines and monoblastic cell line (U937, 67.78%) while it was only weakly positive or negative for other myeloid leukemia cell lines including Meg01 (33.40%), HL-60 (29.70%),K562 (28.19%), KG1a (16.23 %) and HEL92.1.7 (8.02%). Among 4 T lineage leukemia,5 neuroblastoma and 1 colon cancer cell lines tested, only Molt-3 was found weakly positive (31.40%) for 2B8a, while the remaining 3 T cell lines (Molt4, JM and CCRF-CEM), 5 neuroblastoma cell lines (LA-N1, KCNR, BE, SK-N-SH, SK-N-AS) and the colon cancer cell line (HR8348) tested were negative.

结果表明: 2B8a抗原在外周血B细胞上表达(3/3例,平均阳性细胞数为26.29 %),而在T淋巴细胞和NK细胞上不表达(0/3例);在粒细胞和单核细胞上阳性表达均为2/3例,平均阳性细胞数分别是23.72 %和59.84 %;在DC细胞、红细胞和血小板上均不表达(0/3例)。2B8a抗原在骨髓CD34+细胞上的阳性表达是3/3例,平均阳性细胞数39.33 %,而在G-CSF动员的外周血CD34+细胞上的阳性表达仅1/3例,平均阳性细胞数为1.25 %。2B8a抗原在B系细胞系Raji、SMS-SB、Nalm-6和Nall-1上的平均阳性细胞数分别为98.78 %、98.61 %、94.93 %和5.68 %;在T系细胞系Molt-3上的平均阳性细胞数为31.40 %,而在Molt-4、JM和CCRF-CEM 细胞上不表达;在髓系细胞系U937、Meg-01、HL-60、K562、KG1a和HEL92.1.7上的平均阳性细胞数分别为67.78 %、33.40 %、29.70 %、28.19 %、16.23 %和8.02 %;在神经母细胞瘤细胞系SK-N-SH、KCNR、BE、LAN-1和SK-N-AS细胞以及结肠癌细胞系HR8348细胞上均不表达,而在羊膜细胞系FL细胞上呈一定的阳性表达,平均阳性细胞数为45.03%。

Methods test 1 256 samples from suspect uu or ct sufferers and infertile patients during 2007.results the positive rate of uu of 149 samples of prostate liquid is 42.3%(63/149), and the positive rate of ct of 136 samples of prostate liquid s is 19.9%(27/136);the positive rate of uu of 361samples of the secretion of the urinous tract is 21.1%(76/361),and the positive rate of ct of 346 samples of the secretion of the urinous tract is 10.4%(36/346);the positive rate of uu of 141 samples of semen is 11.3%(16/141),and the positive rate of ct of 123 samples of semen is 7.3%(9/123).conclusion there are significant differences in positive rate of uu and ct of 3 types of samples p.005.the positive rate of specimens of postate liquid is higher than that of the secretion of the urinous tract ,the positive rate of semen test is the lowest..the results from the uu and ct test of different types of specimens are different obviously .the most suitable specimen of test is prostate liquid.

对2007年1 256例怀疑感染uu或ct的患者和不孕不育症患者标本检测。结果:149例前列腺标本中uu阳性率为42.3%(63/149)、136例前列腺标本ct阳性率为19.9%(27/136);361例尿道分泌物uu阳性率为21.1%(76/361)、346例尿道分泌物ct阳性率为10.4%(36/346);141例精液uu阳性率为11.3%(16/141)、123例精液ct阳性率为7.3%(9/123)。结论:三种标本的uu、ct阳性率相差显著,p.005。前列腺标本检测的阳性率明显高于尿道分泌物标本,精液检测的阳性率最低。不同类型的标本检测的uu、ct的结果有很大的差异,送检的标本最好是前列腺液。

RESULTS The positive rate of P-gp170expression in cervi-cal cancer pre-radiotherapy was58%(22/38).Among them,the positive expression rates were10/18(56%),6/10,4/6and2/4in keratinizing,nonkeratinizing squamous-cell carci-noma,adenocarcinoma and adeno-squamous-cell carcinoma respectively.The positive rate of post-radiotherapy was90%(34/38)and among them,the positive expression rates were16/18(89%),8/10,6/6and4/4in keratinizing,nonkera-tinizing squamous-cell carcinoma,adenocarcinoma and adeno-squamous-cell carcinoma respectively.

结果 宫颈癌组织P-gp170阳性表达在放疗前为58%(22/38),其中角化型、非角化型鳞癌、腺癌及腺鳞癌分别为10/18(56%),6/10,4/6,2/4例;放疗后其P-gp170阳性表达为90%(34/38),其中角化型、非角化型鳞癌、腺癌及腺鳞癌分别为16/18(89%),8/10,6/6,4/4例,P-gp170表达阳性强度较放疗前亦呈增高趋势。

Results In 136 patients, a total of 72cases sputum culture positive, the positive rate of 52.9% sputum culture for three consecutive single bacteria were 16 cases, the positive rate of 11.8%.16 patients sputum results were:hemolytic streptococcus 6 cases, 4cases of Pseudomonas aeruginosa , Klebsiella pneumoniae in 2 cases and gas production Bacillus 2 cases , Enterobacter Bacillus 2 cases, 136 patients with 386 cases sputum culture times , 106 times positive(27.5%), and consecutive three or more times a train 4 the same bacteria ,only 42 cases(10.8%); the same single-64 cases sputum culture , which amounts to 16.6%.

结果 在136例患者,共72例痰培养阳性,阳性率52.9%,痰培养连续3次为单一细菌者为16例,阳性率为11.8%。16例患者痰菌结果分别为:溶血性链球菌6例,绿脓杆菌4例,克雷伯氏杆菌2例,产气杆菌2例,阴沟杆菌2例,136例患者386例次痰菌培养,106例次阳性(27.5%),而连续3次以上培养为相同一细菌,仅为42例次(10.8%);非相同单一痰菌培养64例次,占16.6%。

The prewashed samples and postwashed samples were analyzed by seminal fluid assay.RESULTS:(1) The positive rate of semen HBV DNA was 21.05%(12/57) with 4.7810~4copies/ml on average; the positive rate of serum HBV DNA was 81.82%(45/55 with 2.3310~7copies/ml on average. There is positive rank correlation between semen HBV DNA copies and serum HBV DNA copies;(2 There were 7 positive cases in 21 samples before washing, the 6 cases except one were positive after washing;(3) there arepositive rank correlation between viability、round cells、 abnormal morphology and semen HBV DNA copies.

结果:(1)精液HBV DNA阳性检出率为21.05%(12/57例),平均拷贝数为4.7810~4/ml,血清HBV DNA阳性检出率为81.82%(45/55例),平均拷贝数为2.33(10~7/ml,精液与血清HBV DNA拷贝数等级间存在正相关关系;(2)21份用于洗涤的精液标本中,7份精液HBV DNA阳性,阳性率为33.33%(7/21例),洗涤后仍有6份标本阳性;(3)精液常规分析显示精子活力、圆细胞数、精子畸形率与精液中HBV DNA载量之间存在正相关关系。

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