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Result: 286 restrain the secretion of TGF-β〓 and decrease the quantity ofα-SMA in liver cells, and the accumulation of FN, LN, type Ⅰand Ⅲ collagen in ECM. The degree of liver cell damage was alleviated and the adverse effect what the purtenance received was lightened. The store function of liver was enhanced.

结果:"268方"可抑制TGF-β〓的分泌,减少α-SMA在肝细胞间存在的量,减少ECM之FN、LN、Ⅰ、Ⅲ型胶原等的沉积,同时可减轻肝细胞受损程度、增强肝脏贮备功能、减轻内脏受到的不良影响,从而实现预防及治疗慢性肝炎的目标。

Result:286 restrain the secretion of TGF- P 1 and decrea se the quantity of a -SMA in liver cells ,and the accumulation of, HSJ.LN, type I and HI collagen in ECM The degree of liver cell damai ie was alleviated and the adverse effect what the purtenance received was lightened The store function of liver was enhanced The preventive and curative effect on chronic hepatitis was re ached.

结果:"268方"可抑制TGF-β_1的分泌,减少α-SMA在肝细胞间存在的量,减少ECM之FN、LN、Ⅰ、Ⅲ型胶原等的沉积,同时可减轻肝细胞受第6hi共96贝成都中医药人学助:1:毕业论义作者:潘年松损程度、增强肝脏贮备功能、减轻内脏受到的不良影响,从而实现预防及治疗慢性肝炎的目标。

Results The detective rates of symptoms as dizziness, sight descend, faintness for seeing, bleed to brush teeth and pain in liver area in the ease group were significantly higher than that in the control groups; The detective rates of lens turbidness, liver augment, Hb descend and abnormity of liver function were also significantly higher than that in the control group.

结果 接触组自觉症状中头昏、视力下降、视物模糊、刷牙出血、肝区疼痛5项的检出率明显高于对照组;体检项目中眼晶体混浊、肝大、血红蛋白降低、肝功能异常4项的检出率明显高于对照组。

The results showed that either of the extracted propolis had significant inhibitory effect on the level of triglyceride, total cholesterol, LDL-C,GPT and GOT in serum, and TC, TG and MDA in liver, but were ineffective on HDL-C, MDA, SOD and NO levels in serum. Ethanol extracted propolis also reduced the body weight, liver weight and liver index of hyperlipidemic SD rats, while water extracted propolis were adiaphorous.

结果表明:蜂胶醇提液和水提液均能有效抑制高脂血症SD大鼠血清中的甘油三酯、总胆固醇、低密度脂蛋白、谷丙转氨酶、谷草转氨酶的升高,并且能有效抑制肝脏组织中的TC、TG、MDA的升高,但对血清中的高密度脂蛋白、超氧化物歧化酶、丙二醛、一氧化氮的影响不明显;蜂胶醇提液能有效降低高脂血症SD大鼠的体重、肝重及肝指数,蜂胶水提液对体重、肝重及肝指数的影响则不显著。

Objective To observe the pathological changes and investigate the hepatocyte apoptosis relating with CYP2E1 in alcoholic liver diseaseof rat. Method the Model of Alcoholic liver Disease: Rats were randomly divided into model group(n=37)and control group(n=33). The dose of 40%ethanol was administerd at 8g/kg body weight by garage twice daily for 8 weeks in model group, and control group was received isovolume saline by gavage. End of 8th week, the activities of serum ALT and AST were detect by autobiochemistry instrument. The pathological changes of liver was observed under light microscope after HE staining, hepatocyte apoptosis was detected by the TUNEL method.

目的: 研究酒精性肝病细胞凋亡程度与细胞色素P4502E1表达的关系方法:用酒精灌胃法制备大鼠酒精性肝病模型,将70只wistar大鼠随机分为正常对照组(33只)和酒精灌胃组(37只),连续灌胃8周后病理切片观察细胞凋亡程度,干化学法测定ALT、AST的含量变化,用PCR法测定细胞色素P450 2E1的表达变化。

The occuurrence of depression is consanguineously related to the weak physique, diffidence, and stagnation of liver qi. As for the pathogenesis, Depression usually has relation with heart, brain, liver, spleen, stomach, the three warmers, kidney, and especially liver, spleen and stomach. The stagnation of qi is the most important character of this disease.

病机方面,认为其病位主要在心、脑,但其发生与肝、脾、胃、三焦、肾和胆等脏腑有关,尤其是与肝脏和脾胃密切相关,气机郁滞是本病最重要的病理特征;在病机转化上,本病大多具有肝失疏泄,气机郁滞,日久郁而化火,导致正虚邪盛的特点。

Nepalensis wall polysaccharide was prepared with hot water extraction,ethanol precipitation,dialyzation,and lyophilization.the crude polysaccharide was separated by deae-cellulose chromatography and purified by sephadex g-200 chromatography.the inhibit effect of the polysaccharide on lipid peroxidation of mice liver was conducted by tba method.the model of liver-injury induced by carbon tetrachloride(ccl4was used to validate the variety of the realated ast.results onefold ingredient polysaccharide was obtained after isolated and purified.conclusion the ingredient can remarkably inhibit lipid peroxidation of rat liver and significantly reduce activity of ast.

用热水提取,乙醇沉淀提取粗多糖,再经sevag法去除蛋白,透析去除小分子物质,冷冻干燥得楮头红多糖粗品;采用deae-52纤维素柱层析和sephadex g-200凝胶柱层析进行分离纯化;用tba法考察多糖的抗脂质过氧化作用;采用ccl4急性肝损伤模型,测定其血清中ast变化。结论获得一个楮头红多糖组分。tba法表明该组分对小鼠自发性脂质过氧化具有显著的抑制作用;在保肝试验中,给药组能明显抑制ast的升高,对肝损伤有一定的保护作用。

The rate is 28.5 percent of abnormal liver function in fatty liver disease,drinking,obesity,high triglyceride,high cholesterol,diabetes and the sugar tolerance unusual,hyperuricaemia,hypertension,hepatic distomiasis,all are the risk factors for fatty liver disease.

通过询问病史、生活史及既往史,测量身高、体重、血压,检测血脂、血尿酸、血糖、胰岛素、肝功能、华支睾吸虫抗体、肝脏B超等,收集2003~2005年连续3年来在我科健康普查的11956名成人的临床资料,进行临床医学统计处理和分析。

The mice inoculated with Coxiella burnetii intrana- sally developed interstitial pneumonia,while the primary pathological changes of mice inoculated intraperitoneally are granulomas in spleen and liver.2.The pathological changes became more severe followed the dosage increasing.3.Coxiella burnetii can be detected in spleen and liver at day 2 after inoculation.the lesion became more and more serious from day 2 to day 12.The characteristic changes were observed at day 7,and recovered at day 14. 4.The reticuloendothelial system are main target of Coxiella burnetii.The pathogen was detected in cytoplasm of monocyte -macrophages of spleen, liver, lung, and endothelioid cells of blood vessel. 5. Coxiella burnetii can be found in macrophages lysosomes by electron microscopy. Most of them are round or rod, and polymorphic shape can also be observed in different size.

结果:1、通过不同感染途径的实验证实,滴鼻感染的小鼠主要表现为间质性肺炎,而腹腔注射感染小鼠则以脾脏、肝脏肉芽肿为主要病变。2、通过不同剂量的感染实验发现,随着感染Q热立克次体剂量的加大,动物病变愈加严重。3、通过感染后不同时间的动态病理学观察发现,在腹腔注射后第2d的脾和肝脏即可发现病原体,主要脏器的病理变化从第2d到第12d逐渐加重,第7d动物的病变最典型,至感染后14d动物的受损器官已开始出现修复性变化。4、 Q热立克次体主要侵害机体的网状内皮系统,在感染小鼠的肝、脾、肺和外周血管单核巨噬细胞以及血管内皮细胞胞浆中查见病原体。5、透射电镜观察可见Q热立克次体主要位于巨噬细胞吞噬溶酶体内,呈多形性,多见圆形和杆状,大小不一。

It will provide the rational for exploring the ideal treatment for liver cancer by detecting the expression of c-Met,hTERT and MAGE-1,which has high expression in liver cancer,in cancer tissues and tissues surrounding tumor by immunohistochemistry,analyzing the different expression of gene in tumor tissue and tissues with different distance from tumor,and then initially debate the molecular boundary in liver tumor exairesis.

通过免疫组织化学方法检测原发性肝细胞癌组织中高表达基因c-Met、hTERT、MAGE-1的表达以及它们在肝癌癌周组织中的表达,分析基因在肝癌组织和癌周不同距离组织的差异表达,初步探讨肝癌的分子切缘,为探索新的治疗肝癌途径奠定坚实的理论基础。

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