查询词典 the limit

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

To confirm your reservation with the ticket 1, the day of booking and the day of the vote送票: In the civil aviation system after successful booking, customer service center will provide you with the confirmation in accordance with the manner with you to confirm the booking of flights and prices, and you need送票time for you送票; the event booking is unsuccessful, call center phone will notify you as soon as possible, so please be sure to keep you filled in Tel flow 2, the first book, not counting: If you would like to book machine bit later on the ticket, please indicate your deadline for the final out of votes (if you requested time limit for the final out of votes and the provisions of airline tickets contravene the time limits, the call center will be promptly notified of your final votes in the time limit), if not to specify you may be required to bear the loss Refund; Because the airlines have the right to cancel any reservations unbilled bit machine, so when you out in the final period of time decided to vote the ticket, please immediately notify the call center phone, we will call the day of your again for you to confirm your reservation slots previously the case.

确认预订与出票 1、当天预订并当天出票送票：在民航系统中订位成功后，客服中心将按照您提供的确认方式与您确认所预订的航班以及价格，并按您所需要的送票时间为您送票；万一订位不成功，客服中心将会尽快电话联系通知您，故请务必保持您所填的联系电话畅通 2、先预订，不出票：如果您希望先预订机位稍后出票，请注明您最后出票期限（如果您要求的最后出票时限与航空公司之规定出票时限抵触时，客服中心会及时通知您最后出票时限），如果不注明您有可能需要承担退票损失；由于航空公司有权取消任何预订未出票的机位，因此当您在最后出票时限内决定出票后，请立即电话通知客服中心，我们会在您来电当天再为您确认一下您先前预订的机位情况。

Erie shares yesterday also went limit, 627,000 single-hand sales will be down to play in the Erie shares on the daily limit.

伊利股份昨天也到极限，627000单手销售额将下降到伊利股份在上玩跌停。

This study tries to develop some clews for the completion of time limit regulations concerning evidence production in taxation litigation . Key words : taxation administrative action time limit for evidence production

文章主要针对税务行政诉讼中与税务机关最密切相关的证据问题，举证时限制度的不完善之处给出一个意欲完善的思路，着眼点在于行政诉讼法立法层面上的分析。

Moreover it delimitates correlative concept such as upper limit and lower limit of virtual operating , low - level and high - level of virtual operating , virtual operating and virtual operating ecosystem , quasi - virtual operating and standard virtual operating , virtual cooperation , physical operating , the strategy of virtual operating and so on

最后，本章对虚拟经营的上限和下限、虚拟经营的低级阶段和高级阶段、虚拟经营和虚拟经营生态系统、准虚拟经营和标准虚拟经营以及虚拟合作、实体经营、虚拟经营战略等相关概念作了界定。

For the sake of the realization through to limit death penalty, and providing for enough of basis of theories that to limit the death penalty, discussing the basis of humanity, the basis of economy, and the basis of purpose and function at penalty, the basis of criminal policy. Explanted the meaning of humanity and how to performance on the death penalty.

为从理论上就死刑的犯罪主体限制提供充分的依据，就死刑的犯罪主体限制的人道性依据、谦抑性依据、刑罚的目的和功能依据及刑事政策依据展开论述。

If one reaches the limit of elasticity in the felt, wood, and metal, and employs the minimum effort to achieve this limit, one gets the crashing fortissimos that may be the real climactic moments of a performance.

如果人们能使毡子、木材、金属的弹性发挥到极致，并且用最小的努力能达到最大的功效，可能真的就在那演奏的一瞬间，人们获得的是绝对的最强音。

Dusk Looking into the sunset I can't help but notice that despite her beauty, a sense of struggle and hopeless surround the sky . Deep inside you realize that this day is gone, and everything that It had brought is lost forever. Every thought,every action,every dream,every hope, every sight,every sound is gone. There is no chance of every being returned the same, exactly the same. For every moment has a limit to what it can capture, Every memory has a limit to what it had retrieve. And the colours in the sky try to entertain us. one last act with painted smiles, for they too know that nothing can be done to save the day. So futile their attempt to comfort our fear of the night. our horror as we try to find our way, like children who wander into a forest and never return. I am ingratiated by the sunset because of her sensitivity as she tries to push the darkness back for just a moment more. But like so many times before....to no avail!

黄昏 凝望夕阳，我无法抑止地看到，无论她是如何的美丽动人，挣扎和绝望依然萦绕天幕，内心深处，你明白今日已逝，它所带来的一切也永远消失了，每一脉思想，每一次行动，每一个梦想，每一线希望每一幅景象，每一缕声音，都消逝而去，一切都不可能还复如初，和原来一模一样，只因每一个时刻所能捕捉的东西是有限的，甚至每一段记忆所能缅怀的一切也是有限的而天空中的绮丽色彩努力让我们快乐起来，最后上演的是五彩缤纷的微笑，因为它们也知晓，做什么都无法留住时日，它们想安慰我们对夜的恐惧，而又如此徒劳无益，虽然恐惧，我们依然寻找出路，宛若在林中的孩心，彷徨不知归路，我满心欢喜，只因夕阳的敏感，她竭力将黑暗推回，仅仅为了再多驻留一刻，可却犹如以往，一切都无济于事。。。。

A discussion about the miniaturing limit of lens is also presented and the miniaturing limit criterion for lens is obtained.

其主要性能指标优于国外同类器件已报导的水平。

The so-called overdrawing with evil intention mentioned in the preceding paragraph means that the card holder, for the purpose of illegal possession, overdraws exceeding the limit of overdrawn account or the time limit, and refuses to return the money after the card-issuing bank demands for the return.

前款所称恶意透支，是指持卡人以非法占有为目的，超过规定限额或者规定期限透支，并且经发卡银行催收后仍不归还的行为。盗窃信用卡并使用的，依照本法第二百六十四条的规定定罪处罚。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"