查询词典 the last stages

Oh, I guess it must be the breast augmentation I got last week.

哦，我想一定是上礼拜做了隆乳的关系。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Last weekend on the small and technical Augsburg track was a round of the south German Nationals.

上周末对小和技术奥格斯堡的轨道是一个一轮的南德国的国民。

Until counting last leaf, can make augur to you and his love.

到数到最后一片叶子的时候，就可以对你和他的爱情作出占卜了。

To the last century 90's, 95% of Staphylococcus aureus have a certain degree of resistance to penicillin.

到上世纪90年代，95%的葡萄球菌都对青霉素有一定的抗药性。

Although you should auscultate the abdomen first,then palpate it ,usually the heart and lungs are auscultated last.

虽然腹部应该先听诊再触诊，但通常心脏和肺应后听诊。我们通常使用听诊器，尽管喘息声不用听诊器也能听见。

Last but not least is the Aussie style Fish and Chips..

最后但并非最不重要的是澳洲风格的炸鱼薯条。。

During last credits, there is a cameo of distinguished american writer Paul Auster.

在上学分，有一个奥斯特客串的著名的美国作家保罗。

OUTSIDE MARKET DEVELOPMENTS:(6:00 AM CST) With the outlook for the Greece situation seemingly improved by the latest austerity program and equity market action generally upbeat over the last 24 hours, that seems to have left physical commodities like the metals markets in favor.

外部市场发展情况：最新严格的救助项目和过去的24小时里股票市场整体向上，看起来对希腊局势前景的看法有所改善，很可能会把力量转向像金属市场的实体商品市场。

In 1916 the last vestiges of the old world disappeared with the death of Franz Josef, Emperor of Austria-Hungary.

约瑟夫的去世而化为灰烬。

A small, rubber-headed hammer used in examination or diagnosis by percussion.

叩诊槌用在通过''。'振荡'。''所作的检查或诊断中的橡皮头的''。'小'。''槌

Affixes are limited in number in a language, and are generally classified into three subtypes, namely, prefix, suffix, and infix, depending on their position with reference to the root or stem of the word.

语言中词缀的数量总是有限的，根据它们跟词根或词干的相对位置，一般可以把词缀分外三小类：前缀，后缀和中缀。