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Seeds were sprouted at 25 ℃.The root-tip with 0.5~1.0 cm length were cut and treated in 4 ℃ refrigerator for 24 hours, fixed for 1~1.5 hours with Carnoy's fixative, differentiated for 10-15 min with hydrochloric acid and Ethanol 95 %(1:1), pressed to section in carmine for staining. Them were observed under microscope, then the samples that differentiated well chosen, covered on frozen station with mountin Canada balsam, for micro-photographing, counting and measuring.

方法]种子25℃萌发,待根尖长0.5~1.0 cm,冰箱4℃冷藏预处理24h,卡诺固定液固定1~1.5h,1:1的浓盐酸和95%乙醇解离10~15min,改良的石碳酸品红液染色,镜检取分散良好的标本,冰冻揭盖片,中性树胶封片显微照像、计数、测量。

The local lesion assay indicated that: contacted with TMV infected leaf juice dilution over 12 h, the disinfestants whose inactivation ratio of TMV above 80% are as follows: 7% sodium hypochlorite at 10 μLL^(-1), 30% chloride of lime at 15 mgL^(-1), 40% Yubao at 75 μLL^(-1) and 98% potassium permanganate at 20 mgL^(-1), 10% chlorine dioxide at 25 mgL^(-1) inactivation ratio of TMV less than 50%.

结果表明,与TMV病叶汁液2万倍稀释液接触12 h,体积分数7%有效氯次氯酸钠10μLL^(-1)、质量分数30%有效氯漂白粉15 mgL^(-1)、体积分数40% N-羟乙基亚甲胺多聚物75μLL^(-1)和质量分数98%高锰酸钾20 mgL^(-1)对TMV的抑制率高于80%,质量分数10%二氧化氯粉剂25 mgL^(-1)对TMV的抑制率低于50%。

The chondrite normalized distribution patterns of REEs are characterized by light REE enrichment and Eu depletion with terrestrial sedime...

约 1 740年以前,陆相特征明显,气候干燥、寒冷,但是,1 5 5 0年左右有一暖期。1 740年后,出现海侵,巴罗气候变暖,1 82 1年后,变暖较明显,1 890年左右为低谷。1 90 4年持续变暖,1 971年左右,有一变冷阶段

Based on analysis of Wx of D4063-1, indica and japonica and according to the special sites of the three species, primers as markers-AUT4063-I were designed to distinguish D4063-1 from other rice. Combining with primer pair F5, dominant and codominant ways were established for discriminating them, and rapid and correct identification of D4063-1 from other rice could be done.

本研究还根据D4063-1和籼稻、粳稻的序列差异及D4063-1在该片段上的特征序列位点设计了用于识别D4063-1的寡核苷酸片段,并作为PCR反应的引物命名为AUT4063-1,将该引物与作者设计的扩增普通籼稻、粳稻Wx基因的引物F5配合使用,建立了识别D4063-1的显性和共显性两种检测方式的分子标记,为快速、准确鉴别低直链淀粉含量突变体D4063-1创造了条件。

Holidays(2001): Jan. 1 (New Year''s Day), Jan. 4 (Commemoration of the Martyrs of Independence), May 1, June 24 (Promulgation of the 1967 Constitution and Day of the fishermen), June 30, Aug. 1 (Parents'' Day), Oct. 14, Nov. 17, Nov. 24 (Anniversary of the Second Republic), Dec. 25.

货 币:1刚果法郎=100马库塔;1美元=49刚果法郎(2000年5月) Currency: 1 Congo Franc=100 new makuta; 1 U.S.dollar=49 Congo Franc (2000.5)节日(2001年);新年(1月1日)、争取独立殉难烈士纪念日(1月4日)、劳动节(5月1日)、1967年宪法颁布日和渔民节(6月24日)、独立日(6月30日)、双亲节(8月1日)、青年节(10月14日)、建军节(11月17日)、第二共和国日(11月24日)、圣诞节

Suppose C is a Graph with k loops andf: G 梸- G is a continuous map with at least a peri- odic point. We prove the following three conditions are equivalent:(1) f is equicontinuous;(2) One of the following End + 2k + 1 conditions holds: in FJm.~0(E~+2L!= flJ1,m = l,2,挃,End J=1 + 2k; 1 0 = flf is a ioop on Gandf I~:0?- 0 is conjugate j=1 to a rational rotation;(3) One of the following End + 2k + 1 conditions holds: k m Lfl.m/~oE~(G+2i!~ is uniformly convergent, m = 1,2,,End+2k; n There is a poistive integer number n,~~~ is uniformly convergent.

假设G是含有k个圈的图,f是G到自身上的连续映射,并且至少有一个周期点,我们证明了下列三条陈述是等价的:(1)f是等度连续的;(2)下列End+2k+1个条件之一成立: m)F l)O=是G的一个圈,且fl_o:O→O共轭于一个有理旋转;(3)下列End+2k+1个条件之一成立: m)是一致收敛的,m=1,2,…,End+2k; l)存在某个正整数n,是一致收敛的。

The expressed protein concentrates were about 0.5-5mg/L. 3. Classification and functional annotation of kinases have been studied: according to the kinases Classification in PlantsP (http://plantsp.genomics.purdue.edu/), 27 kinases belong to 7 groups in the class 1 of Transmembrane Receptor Kinase and Related non-Transmembrane Kinases on PlantsP, namely Receptor like cytoplasmic kinaseⅦof group 1.2, CRPK1 Like Kinase of group 1.3, Crinkly 4_Like kinase of group 1.4, Plant Extemal Response Like Kinase of group 1.6, CRPK1 Like Kinase (Types 1 and ) and S-Domain Kinase (Type 2) of group 1.9, Legume Lectin Domain Kinase of group 1.11, Wall associate kinase of group 1.5, respectively. 4. 21 kinases expressed in S.

对获得的蛋白激酶进行了分类和功能预测:按照PlantsP网站(http://plantsp.genomics.purdue.edu/)的激酶分类系统,本实验表达的激酶分别属于第一类跨膜受体激酶和无跨膜区的激酶中的7个不同组,即第2组的Receptor LikeCytoplasmic KinaseⅦ家族、第3组的CRPK1L(Type 1)家族、第4组的Crinkly 4_Likekinase家族、第6组的Plant External Response Like Kinase家族、第9组的CRPK1 LikeKinase(Types 1 and 2)和S-Domain Kinase(Type 2)家族、第11组的Legume LectinDomain Kinase家族和第17组的Wall Associated Kinase kinase家族; 4。

If the vertex set of a graph can be partitioned into two disjoint nonempty sets V_(1) and V_(2), every vertex in V_(1) connects every vertex in V_(2) and there is no edge in V_(1) and V_(2), then we call this graph is a complete bipartite graph. If V_(1) contains m elements and V_(2) contains n elements, then we denote this complete bipartite graph K_.

当一个图中的点集合可以分成两个非空的集合V_(1)及V_(2),若 V_(1)中的每一点都与V_(2)中的点有边相连,且在V_(1)及V_(2)中的点都没有边相连,则称此图为完全二分图;若 V_(1)中有m个点,V_(2)中有n个点,则此完全二分图记为K_。

We develop and apply the Hirota bilinear-θfunction method,Jacobi elliptic function expansion method,linear superposition method and F-expansion method respectively to solve many 2+1 dimensional nonlinear wave models including 2+1 dimensional 2DsG equation,the coupled ZK equation,2+1 dimensional KdV equation,2+1 dimensional long wave short wave resonance interaction equation and 2+1 dimensional dispersive long wave equation,abundant Jacobi elliptic function doubly periodic solutions are derived.These solutions show various periodic wave shapes and special periodic characters.

发展和应用Hirota双线性-θ函数方法,雅克比椭圆函数展开法,线性叠加法,F-函数展开法等分别求解2+1维2DsG方程,耦合ZK方程,2+1维KdV方程,2+1维长波短波共振相互作用方程,2+1维色散长波方程,获得丰富的雅克比椭圆函数双周期波解,描述了一些周期波形态及周期特性。

Finally, because cnx-1 can further increase the germ cell death in ced-1 mutant, and the phenotype can be rescued by introducing cnx-1::gfp into the cnx-1; ced-1 double mutants, we suggest that cnx-1 acts in the engulfing cells other than the dying cells during the engulfment process of germline apoptosis.

除此之外,我们亦分析了cnx-1突变下ced-1(e1735)品种虫体之生殖母细胞的细胞死亡数量,发现cnx-1突变会额外地再提高ced-1突变下线虫生殖母细胞的死亡数量,且此现象可被外加入之cnx-1::gfp 所消除;这两现象显示出cnx-1在线虫生殖母细胞的细胞尸体吞噬过程亦具有功能。

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And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时,论述了一体化管理体系构建中的几个主要问题,包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。

If you're huffing and puffing at any point during the run, slow down and make sure you can take deep breathes.

如果你任何时候在跑步过程中气喘如牛,降低速度并且确保你能够深呼吸。